The article deals with information about 89 species of aphyllophoroid fungi collected on the territory of the Ussuriysky Nature Reserve in 2020–2021. There are 26 species new for this territory, including Podoscypha petalodes, which is new for Russia and two species new for the Primorye Territory (Antrodiella serpula, Perenniporia medulla-panis). Besides, information about Hyphodontia tropica, recently discovered for the first time in Russia is provided. Both morphological and molecular genetic methods were used for the species identification. In addition, pure cultures were obtained from freshly collected specimens to conserve ex situ macromycete diversity in the Far Eastern region. Thus, to date, the biota of aphyllophoroid fungi in the Ussuriysky Nature Reserve includes 274 species.