Dominance of International High-Risk Clones in Carbapenemase-Producing Pseudomonas aeruginosa: Multicentric Molecular Epidemiology Report from India

Pragasam, Agila Kumari; Veeraraghavan, Balaji; Anandan, Shalini; Narasiman, Vignesh; Sistla, Sujatha; Kapil, Arti; Mathur, Purva; Ray, Pallab; Wattal, Chand; Bhattacharya, Sanjay; Vijayashri, Deotale; Kandasamy, Subramani; Peter, John Victor; Hariharan, T D; Iyyadurai, Ramya; Iniyan, S.; Walia, Kāmini; Ohri, V C

doi:10.4103/ijmm.ijmm_18_294

Cited by 24 publications

(7 citation statements)

References 15 publications

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“…Sequence Types ST1329, ST622, ST1701 and ST357 have been found in Lebanon [3,21] and in different countries, such as Netherlands [52] and Russia [49], whereas the ST253 has never been reported in Lebanon, but has been identified in China [53]. On the other hand, the ST233 that represents the major clone in the hospital has already been described in Lebanon and different countries worldwide [3,21,[54][55][56][57]. This ST233 VIM-2-producing P. aeruginosa, which accounts for the majority of isolates is of great concern as it belongs to the international clone and is now an endemic clone in this hospital.…”

Section: Discussionmentioning

confidence: 99%

Carbapenem and colistin-resistant bacteria in North Lebanon: Coexistence of mcr-1 and NDM-4 genes in Escherichia coli

Al-Bayssari

Dagher

Hamoui

et al. 2021

J Infect Dev Ctries

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Introduction: The increasing incidence of infections caused by multidrug-resistant bacteria is considered a global health problem. This study aimed to investigate this resistance in Gram-negative bacteria isolated from patients hospitalized in North-Lebanon. Methodology: All isolates were identified using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antibiotic susceptibility testing was achieved using disk diffusion, E-test and Broth microdilution methods. Phenotypic detection of carbapenemase was carried out using the CarbaNP test. RT-PCR, standard-PCR and sequencing were performed to detect resistance genes and oprD gene. Conjugal transfer was carried out between our isolates and Escherichia coli J53 to detect the genetic localization of resistance genes. MLST was conducted to determine the genotype of each isolate. Results: Twenty-three carbapenem-resistant Enterobacterales of which eight colistin-resistant Escherichia coli, and Twenty carbapenem-resistant Pseudomonas aeruginosa were isolated. All isolates showed an imipenem MIC greater than 32 mg/mL with MICs for colistin greater than 2 mg/L for E. coli isolates. All the Enterobacterales isolates had at least one carbapenemase-encoding gene, with E. coli isolates coharboring blaNDM-4 and mcr-1 genes. Moreover, 16/20 Pseudomonas aeruginosa harbored the blaVIM-2 gene and 18/20 had mutations in the oprD gene. MLST revealed that the isolates belonged to several clones. Conclusions: We report here the first description in the world of clinical E. coli isolates coharboring blaNDM-4 and mcr-1 genes, and K. pneumoniae isolates producing NDM-6 and OXA-48 carbapenemases. Also, we describe the emergence of NDM-1-producing E. cloacae in Lebanon. Screening for these isolates is necessary to limit the spread of resistant microorganisms in hospitals.

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Section: Discussionmentioning

confidence: 99%

Carbapenem and colistin-resistant bacteria in North Lebanon: Coexistence of mcr-1 and NDM-4 genes in Escherichia coli

Al-Bayssari

Dagher

Hamoui

et al. 2021

J Infect Dev Ctries

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“…In clinical CRE and CRAB isolates, carbapenem resistance is typically conferred by plasmid-acquired carbapenem-hydrolyzing enzymes (carbapenemases) ( 2 , 3 ). In contrast, among clinical CRPsA isolates, carbapenemase positivity rates vary considerably between survey sites: rates are below 5% in most European countries and in North America, while rates of 30% to 70% have been reported from studies in Asia ( 4 – 11 ). Among these carbapenemases, VIM and IMP show the highest prevalence; others such as FIM, GES, GIM, KPC, OXA-48, and SPM are often associated with specific geographic regions or high-risk clones, i.e., clones associated with multidrug resistance and global spread ( 12 ).…”

Section: Introductionmentioning

confidence: 88%

PorinPredict: In Silico Identification of OprD Loss from WGS Data for Improved Genotype-Phenotype Predictions of P. aeruginosa Carbapenem Resistance

et al. 2023

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“…Multilocus sequence typing (MLST) revealed that Pa150 belongs to ST768. ST175, ST235, ST357, and ST664 are high risk clones of P. aeruginosa, with the highest clinical isolation rates in the world [29][30][31]. However, the MDR ST768 clone is rarely reported.…”

Section: Characterization Of Mdr P Aeruginosa Strain Pa150 and Conjug...mentioning

confidence: 99%

Emergence and Transfer of Plasmid-Harbored rmtB in a Clinical Multidrug-Resistant Pseudomonas aeruginosa Strain

et al. 2022

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Multidrug-resistant (MDR) Pseudomonas aeruginosa poses a great challenge to clinical treatment. In this study, we characterized a ST768 MDR P. aeruginosa strain, Pa150, that was isolated from a diabetic foot patient. The minimum inhibitory concentration (MIC) assay showed that Pa150 was resistant to almost all kinds of antibiotics, especially aminoglycosides. Whole genome sequencing revealed multiple antibiotic resistant genes on the chromosome and a 437-Kb plasmid (named pTJPa150) that harbors conjugation-related genes. A conjugation assay verified its self-transmissibility. On the pTJPa150 plasmid, we identified a 16S rRNA methylase gene, rmtB, that is flanked by mobile genetic elements (MGEs). The transfer of the pTJPa150 plasmid or the cloning of the rmtB gene into the reference strain, PAO1, significantly increased the bacterial resistance to aminoglycoside antibiotics. To the best of our knowledge, this is the first report of an rmtB-carrying conjugative plasmid isolated from P. aeruginosa, revealing a novel possible transmission mechanism of the rmtB gene.

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Dominance of International High-Risk Clones in Carbapenemase-Producing Pseudomonas aeruginosa: Multicentric Molecular Epidemiology Report from India

Cited by 24 publications

References 15 publications

Carbapenem and colistin-resistant bacteria in North Lebanon: Coexistence of mcr-1 and NDM-4 genes in Escherichia coli

Carbapenem and colistin-resistant bacteria in North Lebanon: Coexistence of mcr-1 and NDM-4 genes in Escherichia coli

PorinPredict: In Silico Identification of OprD Loss from WGS Data for Improved Genotype-Phenotype Predictions of P. aeruginosa Carbapenem Resistance

Emergence and Transfer of Plasmid-Harbored rmtB in a Clinical Multidrug-Resistant Pseudomonas aeruginosa Strain

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