Dominance of -specific IgG2 subclass in the humoral immune responses of naturally and experimentally infected cattle

Schmeer, N.; Schnorr, K.L.; Perez-Martinez, J.A.; Storz, J.

doi:10.1016/0165-2427(87)90003-1

Cited by 16 publications

(11 citation statements)

References 6 publications

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“…Despite some improvement in diagnostic techniques, our understanding about the prevalence and pathogenetic significance of these infections, succinctly reviewed by Shewen (11) in 1980, has not substantially changed since that time. In cattle, enzyme-linked immunosorbent assay examinations of sera for the antibody against Chlamydia psittaci suggest a high level of exposure to C. psittaci (6,8,10). The application of nested PCR to bovine clinical specimens substantiated such widespread, but mostly clinically inapparent, presumably low-level infections (3,7), similar to the findings for human C. pneumoniae infections (1).…”

supporting

confidence: 62%

Quantitative Detection of Chlamydia psittaci and C. pecorum by High-Sensitivity Real-Time PCR Reveals High Prevalence of Vaginal Infection in Cattle

et al. 2003

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Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in cattle and conforms to the high seroprevalence of anti-Chlamydia antibodies.Over the last 40 years, evidence has accumulated to suggest the ubiquitous presence of infections with intracellular bacteria of the genus Chlamydia in cattle and other livestock species. Despite some improvement in diagnostic techniques, our understanding about the prevalence and pathogenetic significance of these infections, succinctly reviewed by Shewen (11) in 1980, has not substantially changed since that time. In cattle, enzyme-linked immunosorbent assay examinations of sera for the antibody against Chlamydia psittaci suggest a high level of exposure to C. psittaci (6,8,10). The application of nested PCR to bovine clinical specimens substantiated such widespread, but mostly clinically inapparent, presumably low-level infections (3, 7), similar to the findings for human C. pneumoniae infections (1). However, due to high technical demands, these PCR methods were rarely transferred from research settings to systematic epidemiological investigations and diagnostic use. A simple, highly specific, fluorescentprobe-based single-tube LightCycler quantitative PCR (qPCR) platform was recently developed for the detection of Chlamydia DNA. This platform optimizes sample nucleic acid preservation, extraction, and recovery, as well as qPCR methodology, for maximum sensitivity in the detection of Chlamydia (2). This has opened the possibility for the sensitive routine diagnosis of chlamydial infection as well as for systematic epidemiological studies. Such investigations would benefit from both the high sensitivity and the ability to ascertain quantitative differences in chlamydial burdens between animals, which may be required to understand disease mechanisms. In this initial epidemiological application of the Chlamydia qPCR platform, we addressed the question of chlamydial infection of the bovine genital tract in animals that had not previously been exposed to the possibility of sexual transmission of chlamydiae. We report here a high prevalence of genital tract infection with C. psittaci and C. pecorum in clinically normal virgin cattle.A herd of 51 virgin Holstein heifers, 14 to 16 months old, was sampled four times at weekly intervals. These animals were clinically normal, but low-grade vaginitis was common.Vaginal cytobrush specimens (Histobrush; Fisher Scientific, Suwanee, Ga.) were obtained by 10-s rotation in the vaginal vestibulum, brushes were immediately transferred to 400 l of RNA-DNA stabilization reagent (Roche Applied Science, Indianapolis, Ind.) in a 1.5-ml screw-cap microcentrifuge tube, samples were stirred, and the brushes were cut off. Brushes were removed after a 5-min centrifugation at 3,000 ϫ g at room tempera...

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confidence: 62%

Quantitative Detection of Chlamydia psittaci and C. pecorum by High-Sensitivity Real-Time PCR Reveals High Prevalence of Vaginal Infection in Cattle

et al. 2003

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“…Em bovinos, o desenvolvimento da resposta imunológica ocorre principalmente após o abortamento, persistindo por vários meses e depois, com a cronicidade da doença os títulos tendem a cair (Wilsmore et al 1984, Schmeer et al 1987. Nesse estudo, o critério de inclusão foi propriedade com histórico de aborto nos últimos 12 meses.…”

Section: Discussionunclassified

“…A sensibilidade da prova de fixação de complemento é diretamente influenciada pela classe de imunoglobulina envolvida na resposta imune que o animal desenvolve frente à infecção. Em bovinos, a resposta humoral desenvolvida nas infecções por C. abortus são IgG1 e IgG2, mas somente a IgG1 (presente nas primeiras 3-4 semanas pós-infecção) fixa o complemento de cobaio (Schmeer et al 1987, Twomey et al 2006. Apesar de suas limitações, a prova de fixação de complemento tem sido utilizada em países onde existem programas de controle de aborto por C. abortus (Buendía et al 2001, Longbottom & Coulter 2003.…”

Section: Introductionunclassified

Prevalência de anticorpos anti-Chlamydophila spp. em propriedades rurais com histórico de aborto bovino no estado do Paraná

et al. 2009

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“…However, not all animal sera or immunoglobulin isotypes are able to fix complement (Schmeer et al 1987, Kaltenboeck et al 1997. Furthermore, CFT is not feasible with haemolytic or anti-complementary sera (Cross and Clafin 1963).…”

Section: Virus Reviews and Research 15 Nr 2 2010mentioning

confidence: 99%

Dna Detection and Evaluation of Antibodies Against Chlamydophila Felis in Domestic Cats From the Northeast of the State of São Paulo, Brazil

Seki¹,

Carrasco²,

Mineo³

et al. 2010

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Dominance of -specific IgG2 subclass in the humoral immune responses of naturally and experimentally infected cattle

Cited by 16 publications

References 6 publications

Quantitative Detection of Chlamydia psittaci and C. pecorum by High-Sensitivity Real-Time PCR Reveals High Prevalence of Vaginal Infection in Cattle

Quantitative Detection of Chlamydia psittaci and C. pecorum by High-Sensitivity Real-Time PCR Reveals High Prevalence of Vaginal Infection in Cattle

Prevalência de anticorpos anti-Chlamydophila spp. em propriedades rurais com histórico de aborto bovino no estado do Paraná

Dna Detection and Evaluation of Antibodies Against Chlamydophila Felis in Domestic Cats From the Northeast of the State of São Paulo, Brazil

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