Dominant Bacteria and Biomass in the Kuytun 51 Glacier

Xiang, Song; Shang, Tian-Cui; Chen, Yong; Jing, Ze-Fan; Yao, Tandong

doi:10.1128/aem.00915-09

Cited by 43 publications

(40 citation statements)

References 32 publications

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“…The fresh melt-water (10 ml) obtained from the ice core MuztB was used for the determination of total and live biomass by flow cytometric (FCM) analysis by following the previously reported protocols (Xiang et al, 2009c). The 100 µl of diluted sample was added to the known concentration of fluorescent-dyed bead solution Trucount with 100 µl autoclaved and filtered phosphate-buffered saline (PBS, 50 mM; pH 7; containing 1 mM dithiothreitol, DTT) containing either filtered (0.22-µm Nuclepore) PI dye (final concentration 5 µM, Sigma, P4170), cFDA (final concentration: 10 µM, Sigma, F7378) or cFDA/PI mixture.…”

Section: Biomass Analysis and Clone Library Establishment Of The Bactmentioning

confidence: 99%

“…The stained sample mixture was incubated in the dark for 15 min at 22 • C to allow the cells to stain. Samples were analyzed with a FACSCalibur flow cytometer (Becton Dickinson Immunocytometry Systems, San Jose, California, USA) equipped with an air-cooled argon ion laser emitting 15 mW of blue light at 488 nm and with the standard filter setup (see the detailed information in Xiang et al, 2009c). Approximately 400 ml of melt-water was used for DNA extraction.…”

Section: Biomass Analysis and Clone Library Establishment Of The Bactmentioning

confidence: 99%

“…Approximately 400 ml of melt-water was used for DNA extraction. DNA extraction and further clone library establishment procedure were conducted by following the same protocols as previously used in a microbial analysis of the Kuytun 51 Glacier samples (Xiang et al, 2009c). The 16S rRNA gene amplicons used for the establishment of clone libraries from the Muztag Ata Glacier were generated by PCR amplification with the bacterial universal primer pair 8f (5 -AGAGTTTGATCATGGCTCAG) and 1492R (5 -CGGTTACCTTGTTACGACTT) Weisenburg et al, 1991).…”

Section: Biomass Analysis and Clone Library Establishment Of The Bactmentioning

confidence: 99%

“…To initially investigate microbial biogeography in glacial ice over extended scales of time and space, the present study extended earlier preliminary investigations on the glacier surface (Xiang et al, 2009c) and deep snow (Xiang et al, 2009b), and further investigated the composition of bacterial communities along the four ice core profiles extracted from geographically isolated glaciers. First, to investigate the seasonal community changes in glacier ice, four bacterial clone libraries were established by 16S rRNA gene amplification from the ice core MuztB drilled at an elevation 7000-m Muztag Ata Glacier.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Differences in community composition of bacteria in four glaciers in western China

Chen

Xiang

et al. 2010

Biogeosciences

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Abstract. Microbial community patterns vary in glaciers worldwide, presenting unique responses to global climatic and environmental changes. Four bacterial clone libraries were established by 16S rRNA gene amplification from four ice layers along the 42-m-long ice core MuztB drilled from the Muztag Ata Glacier. A total of 151 bacterial sequences obtained from the ice core MuztB were phylogenetically compared with the 71 previously reported sequences from three ice cores extracted from ice caps Malan, Dunde, and Puruogangri. Six phylogenetic clusters Flavisolibacter, Flexibacter (Bacteroidetes), Acinetobacter, Enterobacter (Gammaproteobacteria), Planococcus/Anoxybacillus (Firmicutes), and Propionibacter/Luteococcus (Actinobacteria) frequently occurred along the Muztag Ata Glacier profile, and their proportion varied by seasons. Sequence analysis showed that most of the sequences from the ice core clustered with those from cold environments, and the sequence clusters from the same glacier more closely grouped together than those from the geographically isolated glaciers. Moreover, bacterial communities from the same location or similarlyCorrespondence to: S.-R. Xiang (srxiang@ns.lzb.ac.cn) aged ice formed a cluster, and were clearly separate from those from other geographically isolated glaciers. In summary, the findings provide preliminary evidence of zonal distribution of microbial community, and suggest biogeography of microorganisms in glacier ice.

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Section: Biomass Analysis and Clone Library Establishment Of The Bactmentioning

confidence: 99%

Section: Biomass Analysis and Clone Library Establishment Of The Bactmentioning

confidence: 99%

Section: Biomass Analysis and Clone Library Establishment Of The Bactmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differences in community composition of bacteria in four glaciers in western China

Chen

Xiang

et al. 2010

Biogeosciences

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“…1). The presence of these uncultured psychrophilic and/or psychrotolerant cyanobacteria in the extreme environments has recently been identified only by culture-independent approaches such as 16S rRNA gene clone library screening or automated ribosomal ITS analysis fingerprinting (Wood et al 2008;Xiang et al 2009;Jungblut et al 2010;Schmidt et al 2011;Zeglin et al 2011;Kleinteich et al 2012). Therefore, strain KNUA009 represents the first laboratory culture to be assigned to a group of uncultured cyanobacteria residing in the cryosphere.…”

Section: Discussionmentioning

confidence: 99%

Isolation and description of a globally distributed cryosphere cyanobacterium from Antarctica

Hong¹,

Kim²,

Choi³

et al. 2013

Polar Research

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A previously uncultured cyanobacterium, strain KNUA009, was axenically isolated from a meltwater stream on Barton Peninsula, King George Island, South Shetland Islands, Antarctica. Molecular evidences showed that the isolate belongs to groups of globally distributed cryosphere cyanobacterial clones and this new isolate represents the first laboratory culture to be assigned to these groups. Strain KNUA009 was able to thrive at low temperatures ranging between 5°C and 20°C, but did not survive at temperatures of 25°C and above. As the isolate morphologically resembled Oscillatoria species, it is suggested that this cyanobacterium may represent a new species clade with cold resistance within the genus Oscillatoria

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Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic

Singh

Roy

2015

J. Basic Microbiol.

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Glacier ice and firn cores have ecological and biotechnological importance. The present study is aimed at characterizing bacteria in crustal ice cores from Svalbard, the Arctic. Counts of viable isolates ranged from 10 to 7000 CFU/ml (mean 803 CFU/ml) while the total bacterial numbers ranged from 7.20 × 10(4) to 2.59 × 10(7) cells ml(-1) (mean 3.12 × 10(6) cells ml(-1) ). Based on 16S rDNA sequence data, the identified species belonged to seven species, namely Bacillus barbaricus, Pseudomonas orientalis, Pseudomonas oryzihabitans, Pseudomonas fluorescens, Pseudomonas syncyanea, Sphingomonas dokdonensis, and Sphingomonas phyllosphaerae, with a sequence similarity ranging between 93.5 and 99.9% with taxa present in the database. The isolates exhibited unique phenotypic properties, and three isolates (MLB-2, MLB-5, and MLB-9) are novel species, yet to be described. To the best of our knowledge, this is the first report on characterization of cultured bacterial communities from Svalbard ice cores. We conclude that high lipase, protease, cellulase, amylase, and urease activities expressed by most of the isolates provide a clue to the potential industrial applications of these organisms. These microbes, producing cold-adapted enzymes may provide an opportunity for biotechnological research.

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Dominant Bacteria and Biomass in the Kuytun 51 Glacier

Cited by 43 publications

References 32 publications

Differences in community composition of bacteria in four glaciers in western China

Differences in community composition of bacteria in four glaciers in western China

Isolation and description of a globally distributed cryosphere cyanobacterium from Antarctica

Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic

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