Dominant Components of the Giant Panda Seminal Plasma Metabolome, Characterized by 1H-NMR Spectroscopy

Abstract

As an assisted breeding technique, artificial insemination has become the main effective practical approach in the captive breeding programs of giant panda worldwide. The composition of seminal plasma plays an important role in the success of breeding. The present work is the first attempt to characterize, by proton magnetic resonance spectroscopy (1H-NMR), the metabolome of healthy giant panda seminal plasma. A total of 35 molecules were quantified, with the concentration of 2,3-butanediol being significantly different between individuals younger than 8 years and older than 13 years, and other distinct age-related trends were highlighted by a multivariate analysis. Isopropanol’s concentration was significantly linked to estrus stages. Besides, the variations in the metabolome’s profile during storage were also evaluated. This study may serve as a reference for further research wishing to shed light on the biological mechanisms affecting giant panda sperm’s overall quality and may ultimately lead to novel approaches to giant panda artificial insemination.

Exploring the metabolome of seminal plasma in two different horse types: Light versus draft stallions

“…Other molecules such as hippurate, fumarate, 2‐hydroxyisobutyrate, cystine and valine have also been found in other species and their roles on fertility and offsprings are currently under investigation (Mateo‐Otero et al, 2021 ; Vyvial et al, 2019 ; Zhu et al, 2022 ).…”

Metabolomic Characteristics of Cecum Contents in High-Fat-Diet-Induced Obese Mice Intervened with Different Fibers

“…Following a previous study [ 30 ], we prepared a D 2 O solution for NMR analysis, containing the sodium salt of 3-(trimethylsilyl)-propionic-2,2,3,3-d 4 acid (TSP, 10 mmol/L), a 1 mol/L phosphate buffer and NaN 3 (2 mmol/L) in. TSP and NaN 3 were included in the solution to serve as an NMR spectra chemical-shift reference and against microbial proliferation, respectively.…”

Metabolomic Analysis of Multiple Biological Specimens (Feces, Serum, and Urine) by 1H-NMR Spectroscopy from Dairy Cows with Clinical Mastitis

“…Among the techniques for metabolomic investigations, 1 H-NMR spectroscopy is considered one of the most used, because it is based on very simple procedures for sample preparation, it is intrinsically non-invasive and strongly quantitative, and it is characterized by high reproducibility and superb instrument stability, which counterbalance a sensitivity lower than other platforms, such as GC-MS [14]. For these reasons, 1 H-NMR spectroscopy has been used in studies about domestic animals to acquire metabolomic profiles of numerous biofluids, for instance urine [15], plasma [16], serum [17], feces [18], tracheal wash, exhaled breath condensate [19], and seminal plasma [20].…”