Dominant‐negative form of the Pax6 homolog <i>eyeless</i> for tissue‐specific loss‐of‐function studies in the developing eye and brain in <i>drosophila</i>

Niimi, Teruyuki; Clements, Jason; Gehring, W.J.; Callaerts, Patrick

doi:10.1002/gene.10140

Cited by 11 publications

(12 citation statements)

References 13 publications

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“…We also analyzed the effect of ey DN on eye imaginal discs and observed the same phenotype as that reported by Niimi et al (Niimi et al, 2002); expression of ey DN resulted in underproliferation of eye imaginal disc cells and a partial or complete loss of eye structures (data not shown), which is similar to what is seen in eyeless mutants. Thus, the effects of ey DN in the eye disc are, surprisingly, the opposite of what we observe in medulla neuroblasts.…”

Section: Discussionsupporting

confidence: 76%

“…The following lines were used for the study: (Evans et al, 2009), shg-lacZ, UAS-p35, UAS-UEE3 and UAS-UEE7 (referred as ey DN ) (Niimi et al, 2002), tub-Gal80 ts , UAS-shg-GFP, UAS-CD8-GFP, UAS-H2B-YFP, UAS-nuGFP and FRTG13 shg…”

Section: Fly Stocksmentioning

confidence: 99%

“…Thus, we addressed the function of ey in neuroblasts. For this purpose, we expressed a dominant-negative form of the Eyeless protein (hereafter referred as ey DN ) in which the N-terminal domain is replaced by an Engrailed-repressor domain (Niimi et al, 2002), rendering the Ey activator a constitutive transcriptional repressor.…”

Section: Altered Migration Of Optic Lobe Cells Lacking Eyeless Functionmentioning

confidence: 99%

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Cell migration inDrosophilaoptic lobe neurons is controlled byeyeless/Pax6

2011

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SUMMARYIn the developing Drosophila optic lobe, eyeless, apterous and distal-less, three genes that encode transcription factors with important functions during development, are expressed in broad subsets of medulla neurons. Medulla cortex cells follow two patterns of cell movements to acquire their final position: first, neurons are arranged in columns below each neuroblast. Then, during pupation, they migrate laterally, intermingling with each other to reach their retinotopic position in the adult optic lobe. eyeless, which encodes a Pax6 transcription factor, is expressed early in progenitors and controls aspects of this cell migration. Its loss in medulla neurons leads to overgrowth and a failure of lateral migration during pupation. These defects in cell migration among medulla cortex cells can be rescued by removing DE-Cadherin. Thus, eyeless links neurogenesis and neuronal migration.

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Section: Discussionsupporting

confidence: 76%

Section: Fly Stocksmentioning

confidence: 99%

Section: Altered Migration Of Optic Lobe Cells Lacking Eyeless Functionmentioning

confidence: 99%

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Cell migration inDrosophilaoptic lobe neurons is controlled byeyeless/Pax6

2011

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“…For example, Pax2 null Zebrafish noi mutants fail to develop an isthmus (Brand et al, 1996), while Pax3 mutants have significant neural crest and neural tube/CNS defects (Auerbach, 1954;Wildhardt et al, 1996) and Pax5 knockout mice exhibit defects in the midbrain-hindbrain region (Urbanek et al, 1994(Urbanek et al, , 1997. Similarly, Pax6 mutations result in severe defects in forebrain development and eye formation (Hill et al, 1991;Jordan et al, 1992;Callaerts et al, 1997;Mastick et al, 1997;Niimi et al, 1999Niimi et al, , 2002 while Pax7 knockout mice exhibit craniofacial defects (Hill et al, 1996;Mansouri et al, 1996b). Although hypomorphic mutant studies are beginning to elucidate a mode of action for Pax genes, earlier studies of Pax mutant animals were to some extent hindered by compensation through gene expression of other Pax family members, and, did not, we believe, fully characterize the role of individual Pax genes (Relaix et al, 2004;Zhou et al, 2008).…”

Section: Pax Expression During Developmentmentioning

confidence: 99%

Perplexing Pax: From puzzle to paradigm

Blake

Thomas

Thompson

et al. 2008

Developmental Dynamics

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Pax transcription factors are critical for the development of the central nervous system (CNS) where they have a biphasic role, initially dictating CNS regionalization, while later orchestrating differentiation of specific cell subtypes. While a plethora of expression, misexpression, and mutation studies lend support for this argument and clarify the importance of Pax genes in CNS development, less well understood, and more perplexing, is the continued Pax expression in the adult CNS. In this article we explore the mechanism of action of Pax genes in general, and while being cognizant of existing developmental data, we also draw evidence from (1)

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“…The ey-GAL4 driver recapitulates expression in the eye field. The antennal part of the joint eye-antennal disc is not affected (Halder et al 1998;Niimi et al 2002). (D) The gray columns indicate counts of dorso-ventral rows of photoreceptor clusters that were labeled with anti-Elav in the same 40 eye discs.…”

mentioning

confidence: 99%

The Chromatin-Remodeling Protein Osa Interacts With CyclinE in Drosophila Eye Imaginal Discs

et al. 2010

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Coordinating cell proliferation and differentiation is essential during organogenesis. In Drosophila, the photoreceptor, pigment, and support cells of the eye are specified in an orchestrated wave as the morphogenetic furrow passes across the eye imaginal disc. Cells anterior of the furrow are not yet differentiated and remain mitotically active, while most cells in the furrow arrest at G 1 and adopt specific ommatidial fates. We used microarray expression analysis to monitor changes in transcription at the furrow and identified genes whose expression correlates with either proliferation or fate specification. Some of these are members of the Polycomb and Trithorax families that encode epigenetic regulators. Osa is one; it associates with components of the Drosophila SWI/SNF chromatin-remodeling complex. Our studies of this Trithorax factor in eye development implicate Osa as a regulator of the cell cycle: Osa overexpression caused a small-eye phenotype, a reduced number of M-and S-phase cells in eye imaginal discs, and a delay in morphogenetic furrow progression. In addition, we present evidence that Osa interacts genetically and biochemically with CyclinE. Our results suggest a dual mechanism of Osa function in transcriptional regulation and cell cycle control.

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Dominant‐negative form of the Pax6 homolog eyeless for tissue‐specific loss‐of‐function studies in the developing eye and brain in drosophila

Cited by 11 publications

References 13 publications

Cell migration inDrosophilaoptic lobe neurons is controlled byeyeless/Pax6

Cell migration inDrosophilaoptic lobe neurons is controlled byeyeless/Pax6

Perplexing Pax: From puzzle to paradigm

The Chromatin-Remodeling Protein Osa Interacts With CyclinE in Drosophila Eye Imaginal Discs

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