Donor/acceptor nanoparticle pair-based singlet oxygen channeling homogenous chemiluminescence immunoassay for quantitative determination of bisphenol A

Abstract

Bisphenol A (BPA) is widely used in consumer products such as plastic bottles and food containers. It has become a ubiquitous environmental contaminant and poses a serious risk to human health. A rapid, sensitive, and highthroughput method for detecting BPA is therefore desirable. Herein, a donor/acceptor nanoparticle pair-based singlet oxygen channeling chemiluminescence homogenous immunoassay is developed for the determination of BPA. The donor nanoparticles were modified with phthalocyanine as a photosensitizer and were then coated with streptavidin. The acceptor nanoparticles were doped with thioxene derivatives and Eu(III) as a chemiluminescence emitter and then coated with anti-BPA antibody. Under light irradiation, oxygen near the donor surface transforms to singlet oxygen ( 1 O 2 ), which migrates to the acceptor and reacts with it, generating luminescence. Because 1 O 2 has a very short lifetime, luminescence is generated only when the donor and acceptor are in close proximity. This occurs when they are brought together by the antigen/antibody and streptavidin/biotin reaction. Based on this singlet oxygen channeling mechanism, a competitive homogenous chemiluminescence immunoassay for BPA was developed on 384 microplates. The assay exhibited linear detection over the range 10-1000 ng/mL and a limit of detection of 2.9 ng/mL. The intra-and inter-assay precisions were both below 5.1 %. The average recoveries of three spiked samples in tap and river water samples were in the range 95.5-121.0 %, in agreement with values obtained using highperformance liquid chromatography. The homogeneous assay is rapid, low cost, sensitive, and allows high-throughput, so is well suited for screening large numbers of environmental samples.

Quantitation of estradiol by competitive light‐initiated chemiluminescent assay using estriol as competitive antigen

“…When the distance is within 200 nm, singlet oxygen will transfer from the donor beads to the acceptor beads under excitation, which will cause the acceptor beads to fluoresce at 520‐618 nm. This is a homogeneous method that is sensitive, specific, stable, and free of separation and washing steps and has high throughput . Because the concentration of E2 varies greatly in different periods, the detection method requires a large detection interval to meet the clinical needs.…”

Multiplexed Luminescence Oxygen Channeling Immunoassay Based on Dual‐Functional Barcodes with a Host–Guest Structure: A Facile and Robust Suspension Array Platform

“…The limit of detection (LOD) was estimated to be 55.6 pg mL −1 following the 3σ criteria, and the dynamic range of 3.5 logs was defined, which were comparable to previously reported singleplexed LOCI approaches. [ 16,19,39 ] It should be noted that when the IFN‐γ concentration was higher than 100 ng mL −1 , the LOCI signal gradually decreased with further concentration increments due to the “hook” effect, which mainly results from the reduced amount of sandwich complexes caused by the high level of analytes. [ 40 ] Mean intensities of QDH@ABs–DBs on barcode channels were also calculated, and they are plotted in Figure 3C.…”

“…[ 11–13 ] Among these methods, LOCI, a commercialized product known as AlphaLISA further developed by PerkinElmer, has been used in a wide range of applications in singleplexed analyte immunoassays over recent years, owing to its high sensitivity and repeatability. [ 14–18 ] LOCI is an assay approach in which the analyte of interest bridges antibody‐coated donor beads (DBs) and acceptor beads (ABs) into a close proximity via forming immunocomplexes. In this state, laser irradiation on DBs at 680 nm generates a flow of singlet oxygen, triggering a cascade of chemical events in nearby ABs, which provokes a chemiluminescent emission at 615 nm.…”

Desenvolvimento de procedimentos analíticos explorando microextração líquido-líquido em fluxo para a determinação de espécies de interesse em águas e leite

“…As discrepâncias em relação às concentrações de BPA em água da torneira de diferentes países [135,148] são provavelmente decorrentes do tipo de tratamento de água e esgoto, porque o BPA não é removido pelos tratamentos convencionais [161,162]. Também devem ser considerados o controle com relação ao uso de polímeros contendo BPA em embalagens de alimentos e bebidas e o tipo de tubulação utilizada no transporte de águas de abastecimento…”

“…Outras técnicas utilizadas para a determinação do BPA exploram ensaios imunoenzimáticos (ELISA) [146,147], quimiluminescência [148], imunoensaio de fluorescência [149] e imunoensaio de polarização de fluorescência [150]. Estas técnicas apresentam limite de detecção na ordem de µg L -1 [147] e ng L -1 [149].…”