Donor-matched comparison of chondrogenic progenitors resident in human infrapatellar fat pad, synovium, and periosteum - implications for cartilage repair

Mantripragada, V.R.; Piuzzi, Nicolás S.; Bova, Wesley; Boehm, Cynthia; Obuchowski, Nancy A.; Lefebvre, Véronique; Midura, Ronald J.; Muschler, George F.

doi:10.1080/03008207.2019.1611795

Cited by 21 publications

(35 citation statements)

References 66 publications

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“…Nevertheless, a number of concerns should be addressed. For example, increasing evidence suggests that the cell population obtained by culture expansion is not homogeneous to MSC cell population [20]. As such, reproducible therapeutic efficiency could be compromised by this cell population manufactured.…”

Section: Discussionmentioning

confidence: 99%

“…As such, reproducible therapeutic efficiency could be compromised by this cell population manufactured. Clonal selection and isolation of the heterogeneous cell population from the connective tissues is regarded as an optimizing means to culture expanded cells harvested from patients [20,80]. Notably, the role of MSCs in the cartilage regeneration is attributable to the paracrine activity of exosomes.…”

Section: Discussionmentioning

confidence: 99%

“…Notably, the role of MSCs in the cartilage regeneration is attributable to the paracrine activity of exosomes. MSCIFP-derived exosomes could protect cartilage regeneration, inhibit the chondrocyte apoptosis and balance the anabolic and catabolic processes [20]. In addition, MSCs-derived exosomes are more stable than MSCs themselves and have immune privileged to a certain degree.…”

Section: Discussionmentioning

confidence: 99%

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Infrapatellar Fat Pad and Knee Osteoarthritis

et al. 2020

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Osteoarthritis is the most prevalent arthritis typically characterized by degradation of cartilage. However, its pathogenesis is not fully understood. Currently, osteoarthritis is best considered a disease of the whole "joint organ". Infrapatellar fat pad (IFP), an adipose tissue near synovium, is now attaching importance to researchers for its inflammatory phenotype. In this narrative review, a large body of evidence has been gathered for the involvement of IFP in the development of knee osteoarthritis. Additionally, the underlying mechanisms of how IFP can be involved in this process have been proposed. However, further investigations are needed to better understand its precise role in this process and its underlying mechanism, and beyond that, to develop new strategies to slow down the degenerative process and explore an effective and timely diagnosis of the disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Infrapatellar Fat Pad and Knee Osteoarthritis

et al. 2020

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“…All 12 included articles were controlled single-institution studies (Table 2). Of the 12 isolated studies, 11 were retrospective [23][24][25][26][27][28][29][30][31][32][33] and one was a prospective cohort study [34]. There was a total of 228 patient IFPs assessed in the 12 studies.…”

Section: Included Studiesmentioning

confidence: 99%

“…The patient ages ranged between 17 and 89. From the six studies indicating gender [23][24][25]27,31,34], 77 (62%) patients were female and 48 (38%) were male.…”

Section: Included Studiesmentioning

confidence: 99%

Culture Time Needed to Scale up Infrapatellar Fat Pad Derived Stem Cells for Cartilage Regeneration: A Systematic Review

2020

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Adipose tissue is a rich source of stem cells, which are reported to represent 2% of the stromal vascular fraction (SVF). The infrapatellar fat pad (IFP) is a unique source of tissue, from which human adipose-derived stem cells (hADSCs) have been shown to harbour high chondrogenic potential. This review aims to calculate, based on the literature, the culture time needed before an average knee articular cartilage defect can be treated using stem cells obtained from arthroscopically or openly harvested IFP. Firstly, a systematic literature review was performed to search for studies that included the number of stem cells isolated from the IFP. Subsequent analysis was conducted to identify the amount of IFP tissue harvestable, stem cell count and the overall yield based on the harvesting method. We then determined the minimum time required before treating an average-sized knee articular cartilage defect with IFP-derived hADSCs by using our newly devised equation. The amount of fat tissue, the SVF cell count and the stem cell yield are all lower in arthroscopically harvested IFP tissue compared to that collected using arthrotomy. As an extrapolation, we show that an average knee defect can be treated in 20 or 17 days using arthroscopically or openly harvested IFP-derived hADSCs, respectively. In summary, the systematic review conducted in this study reveals that there is a higher amount of fat tissue, SVF cell count and overall yield (cells/volume or cells/gram) associated with open (arthrotomy) compared to arthroscopic IFP harvest. In addition to these review findings, we demonstrate that our novel framework can give an indication about the culture time needed to scale up IFP-derived stem cells for the treatment of articular cartilage defects based on harvesting method.

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