Donors with a rare pheno (geno) type

Reesink, H. W.; Engelfriet, C. P.; Schennach, Harald; Gassner, Christoph; Wendel, Silvano; Fontão-Wendel, Rita; Brito, Marina Angelica Magalhães de; Sistonen, Pertti; Matilainen, J.; Peyrard, Thierry; Pham, Bach-Nga; Rouger, Ph.; Pennec, P.Y. Le; Flegel, Willy A.; Zabern, Inge von; Lin, Che Kit; Tsoi, W. C.; Hoffer, I.; Barotine-Toth, K.; Joshi, Sanmukh R; Vasantha, K; Yahalom, Vered; Asher, Orna; Levene, C.; Villa, Maria Antonietta; Revelli, Nicoletta; Greppi, N.; Marconi, Maurizio; Tani, Yoshihiko; Folman, Claudia C.; Haas, Masja de; Koopman, M. M. W.; Beckers, Erik A.M.; Gounder, Dhana; Flanagan, Peter; Wall, Luke A.; Urtasun, Erkuden Aranburu; Hustinx, Hein; Niederhauser, Christoph; Flickinger, Cynthia; Nance, Sandra; Meny, Geralyn M.

doi:10.1111/j.1423-0410.2008.01084.x

Cited by 51 publications

(46 citation statements)

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“…Rare blood is defined, on the basis of the blood group characteristics, as being found at a frequency of ≤ 1:1000 random samples in a given population. 18,19 The Rh phenotype R 2 R Z , r''r, r''r'' and r'r'' were found to be rare in our population with percentage prevalence of 0.07, 0.03, 0.03 and 0.03, respectively. Weak D phenotype was not observed in our study.…”

Section: 11mentioning

confidence: 68%

Frequency of Rh Phenotypes in Voluntary Blood Donors

Pachaury¹

2017

JMSCR

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Section: 11mentioning

confidence: 68%

Frequency of Rh Phenotypes in Voluntary Blood Donors

Pachaury¹

2017

JMSCR

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“…As the allele distribution can differ widely in some populations, there are special needs in different regions of the world. For example, the data of the rare blood supplied in France during a 14-year period from 1994 to 2008 showed demand for: Fy(a-b-) (26.1%), k-(19.4%), Yt(a-) (12.7%), r'r' (9.1%), U-(7.0%), Vel-(5.6%), r'r' (3.5%), Lu(b-) (3.2%), D--(1.1%), Lu:-13 (0.9%), Js(b-) (0.9%), Kp(b-) (0.9%), R z R z (0.7%), I-(0.5%), O h (0.4%) and Ge: -2.3 (0.3%) [2]. Nevertheless, there were shortages in availability for the provision of some types specifically found in the African-Caribbean population (U-D-, hr .…”

Section: Discussionmentioning

confidence: 99%

“…These exemplary data represent the regional aspect in the need for rare blood and indicate that screening for the 'most needed' rare blood groups differs from region to region. In an international comparative survey it was concluded that in general K 0 , McLeod, p, U-, Lan-, Vel-, and Ge:-2,-3 are probably the most difficult phenotypes to find [2].…”

Section: Discussionmentioning

confidence: 99%

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Molecular Bases and Genotyping for Rare Blood Types

Jungbauer¹

2009

Transfus Med Hemother

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The provision of suitable blood units for patients carrying clinically significant antibodies to high-frequency antigens (HFAs) is a special challenge for blood establishments. Typing of donors and screening for HFA-negative individuals is increasingly performed by genotyping. In this context the selection of the HFAs of interest, the molecular background of some model antigens, and the different requirements for donor screening versus resolving serological problems are addressed. In addition, several published approaches for mass-scale donor genotyping are reviewed. Furthermore, the results of a DNA-based donor screening for 12 HFAs in 11,400 Austrian donors that resulted in finding 94 newly identified HFA-negative donors are referred to.

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“…In some European countries like Germany, such alleles are nearly absent, and compatible units can usually be identified by serologic methods. In these countries, the major challenge is the provision of blood for patients with antibodies to the high-prevalence antigens Kp b , Lu b , Yt a or Vel [14], and for patients with multiple alloantibodies [15,16] to the clinical relevant antigens outside ABO and RH. Most of these antigens may be easily determined by serology; hence, any molecular method has to compete with serologic approaches.…”

Section: Introductionmentioning

confidence: 99%

Extended Donor Typing by Pooled Capillary Electrophoresis: Impact in a Routine Setting

Wagner

Doescher

Bittner

et al. 2018

Transfus Med Hemother

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Background: PCR with sequence-specific priming using allele-specific fluorescent primers and analysis on a capillary sequencer is a standard technique for DNA typing. We aimed to adapt this method for donor typing in a medium-throughput setting. Methods: Using the Extract-N-Amp PCR system, we devised a set of eight multiplex allele-specific PCR with fluorescent primers for Fy^a/Fy^b, Jk^a/Jk^b, M/N, and S/s. The alleles of a gene were discriminated by the fluorescent color; donor and polymorphism investigated were encoded by product length. Time, cost, and routine performance were collated. Discrepant samples were investigated by sequencing. The association of new alleles with the phenotype was evaluated by a step-wise statistical analysis. Results: On validation using 312 samples, for 1.1% of antigens (in 5.4% of samples) no prediction was obtained. 99.96% of predictions were correct. Consumable cost per donor were below EUR 2.00. In routine use, 92.2% of samples were successfully predicted. Discrepancies were most frequently due to technical reasons. A total of 11 previously unknown alleles were detected in the Kell, Lutheran, and Colton blood group systems. In 2017, more than 20% of the RBC units prepared by our institution were from donors with predicted antigen status. A steady supply of Yt(a-), Co(a-) and Lu(b-) RBC units was ensured. Conclusions: Pooled capillary electrophoresis offers a suitable alternative to other methods for extended donor DNA typing. Establishing a large cohort of typed donors improved transfusion support for patients.

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Donors with a rare pheno (geno) type

Cited by 51 publications

References 0 publications

Frequency of Rh Phenotypes in Voluntary Blood Donors

Frequency of Rh Phenotypes in Voluntary Blood Donors

Molecular Bases and Genotyping for Rare Blood Types

Extended Donor Typing by Pooled Capillary Electrophoresis: Impact in a Routine Setting

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