Dopamine and senescence in Drosophila melanogaster☆

Neckameyer, Wendi S.; Woodrome, Stacey; Holt, Bridgette; Mayer, Adam

doi:10.1016/s0197-4580(99)00109-8

Cited by 77 publications

(89 citation statements)

References 26 publications

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“…Additional variables include sex and age. Thus, Neckameyer et al (Neckameyer et al, 2000) report more dopamine in males than females, and in younger flies than older. These values refer to wholebody determinations of dopamine, however, not to heads, and although our samples were from 10 flies, our determinations are Wild-type, ebony and double-mutant white; ebony heads.…”

Section: Dopamine and Serotonin Content In The Drosophila Headmentioning

confidence: 97%

DrosophilaABC transporter mutantswhite,brownandscarlethave altered contents and distribution of biogenic amines in the brain

Borycz

Kubów

Lloyd

et al. 2008

Journal of Experimental Biology

149

169

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SUMMARYMonoamines such as dopamine, histamine and serotonin (5-HT) are widely distributed throughout the brain of the fruit fly Drosophila melanogaster, where many of their actions have been investigated. For example, histamine is released from photoreceptor synapses in the lamina neuropile of the visual system. Mutations of the genes white, an important eye pigmentation marker in fly genetics that encodes an ABC transporter, and its binding partner brown, cause neural phenotypes not readily reconciled solely with actions in eye pigmentation. We find that flies mutant for these genes, and another binding partner, scarlet, have about half the wild-type amount of histamine in the head, as well as reduced 5-HT and dopamine. These differences parallel reductions in immunoreactivity to the corresponding biogenic amines. They also correlate with the amine content of fractions after differential centrifugation of head homogenates. Thus, most of the amine is found in the vesicle-rich fraction of wild-type head homogenates, whereas it is found in the supernatant fractions from white, brown and scarlet flies. White co-expresses in lamina epithelial glia with Ebony, which conjugates histamine to β-alanine. Histamine is then released when the conjugate is hydrolyzed in photoreceptors, by Tan. Mutant white ameliorates the effects of tan on head histamine whereas it exacerbates the effects of ebony. Our results are consistent with the proposal that histamine uptake by the epithelial glia may be white dependent. Behavioral abnormalities in white, brown and scarlet mutants could arise because aminergic neurons in the Drosophila brain have reduced amine for release.

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Section: Dopamine and Serotonin Content In The Drosophila Headmentioning

confidence: 97%

DrosophilaABC transporter mutantswhite,brownandscarlethave altered contents and distribution of biogenic amines in the brain

Borycz

Kubów

Lloyd

et al. 2008

Journal of Experimental Biology

149

169

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“…Studies of courtship tend to focus more on the male, the more obvious active party, yet the female's acceptance ultimately determines the male's mating success. 35,36 Age affects female attractiveness to males, as well as female behaviors associated with courtship, receptivity, and mating success 35,37 and reviewed in. 38 While male courtship vigor toward active (intact) females has not been observed to change with increasing female age (5 d-20 d post-eclosion, hereafter abbreviated as d pe; 37 ), male discrimination based on female age is detected under conditions where some female stimuli are isolated.…”

Section: Courtship and Matingmentioning

confidence: 99%

“…35,36 Age affects female attractiveness to males, as well as female behaviors associated with courtship, receptivity, and mating success 35,37 and reviewed in. 38 While male courtship vigor toward active (intact) females has not been observed to change with increasing female age (5 d-20 d post-eclosion, hereafter abbreviated as d pe; 37 ), male discrimination based on female age is detected under conditions where some female stimuli are isolated. When females are immobilized and decapitated to prevent rejection responses, males spend »50% less time courting old females than young females (female ages 20 d-1 d pe, 36 ; 52 d vs 8 d pe, 39 ).…”

Section: Courtship and Matingmentioning

confidence: 99%

The song of the old mother: Reproductive senescence in female drosophila

Miller

Obrik-Uloho

Phan

et al. 2014

Fly

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Among animals with multiple reproductive episodes, changes in adult condition over time can have profound effects on lifetime reproductive fitness and offspring performance. The changes in condition associated with senescence can be particularly acute for females who support reproductive processes from oogenesis through fertilization. The pomace fly Drosophila melanogaster is a well-established model system for exploring the physiology of reproduction and senescence. In this review, we describe how increasing maternal age in Drosophila affects reproductive fitness and offspring performance as well as the genetic foundation of these effects. Describing the processes underlying female reproductive senescence helps us understand diverse phenomena including population demographics, conditiondependent selection, sexual conflict, and transgenerational effects of maternal condition on offspring fitness. Understanding the genetic basis of reproductive senescence clarifies the nature of life-history trade-offs as well as potential ways to augment and/or limit female fertility in a variety of organisms.

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“…To image dopaminergic neurons, WT and dVMAT mutant wandering third-instar larvae were dissected and fixed in 4% paraformaldeheyde and then probed with rabbit anti-DTH (Neckameyer et al 2000), a gift of W. Neckameyer (St. Louis University School of Medicine), followed by antirabbit Alexaflour 488 (Invitrogen Molecular Probes, Eugene, OR). For serotonergic and octopaminergic neuron counts, one copy of UAS-mCD8-GFP and one copy of either TrH-Gal4 or Tdc2-Gal4, respectively, were expressed either in the WT or in the dVMAT mutant background, and the endogenous fluorescent signal of GFP was used to count cell bodies in larval brains from wandering third-instar larvae fixed in 4% paraformaldehyde.…”

Section: Anatomical Analysismentioning

confidence: 99%

“…To determine whether this could be due to gross changes in neural development, we quantified the number of larval aminergic neurons in the mutant vs. WT nervous system ( Figure S2). We used an antibody to tyrosine hydroxylase to detect DA neurons (Neckameyer et al 2000). Of the seven DA clusters we analyzed, one (DL2) showed an 17% decrease in total cell number ( Figure S2, A and B).…”

Section: Rescue Strategymentioning

confidence: 99%

Dispensable, Redundant, Complementary, and Cooperative Roles of Dopamine, Octopamine, and Serotonin inDrosophila melanogaster

Chen

Lebestky

et al. 2013

Genetics

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To investigate the regulation of Drosophila melanogaster behavior by biogenic amines, we have exploited the broad requirement of the vesicular monoamine transporter (VMAT) for the vesicular storage and exocytotic release of all monoamine neurotransmitters. We used the Drosophila VMAT (dVMAT) null mutant to globally ablate exocytotic amine release and then restored DVMAT activity in either individual or multiple aminergic systems, using transgenic rescue techniques. We find that larval survival, larval locomotion, and female fertility rely predominantly on octopaminergic circuits with little apparent input from the vesicular release of serotonin or dopamine. In contrast, male courtship and fertility can be rescued by expressing DVMAT in octopaminergic or dopaminergic neurons, suggesting potentially redundant circuits. Rescue of major aspects of adult locomotion and startle behavior required octopamine, but a complementary role was observed for serotonin. Interestingly, adult circadian behavior could not be rescued by expression of DVMAT in a single subtype of aminergic neurons, but required at least two systems, suggesting the possibility of unexpected cooperative interactions. Further experiments using this model will help determine how multiple aminergic systems may contribute to the regulation of other behaviors. Our data also highlight potential differences between behaviors regulated by standard exocytotic release and those regulated by other mechanisms. BOTH invertebrate and mammalian behaviors undergo extensive regulation by monoamine neurotransmitters (reviewed in Joshua et al.

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Dopamine and senescence in Drosophila melanogaster☆

Cited by 77 publications

References 26 publications

DrosophilaABC transporter mutantswhite,brownandscarlethave altered contents and distribution of biogenic amines in the brain

DrosophilaABC transporter mutantswhite,brownandscarlethave altered contents and distribution of biogenic amines in the brain

The song of the old mother: Reproductive senescence in female drosophila

Dispensable, Redundant, Complementary, and Cooperative Roles of Dopamine, Octopamine, and Serotonin inDrosophila melanogaster

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