Dopamine D1 receptor agonist alleviates acute lung injury via modulating inflammatory responses in macrophages and barrier function in airway epithelial cells

Meng, Linlin; Wang, Muyun; Gao, Yixuan; Chen, Liangzhi; Wang, Kun; Liu, Qinghua

doi:10.1016/j.freeradbiomed.2023.03.016

Cited by 11 publications

(6 citation statements)

References 57 publications

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“…THP-1 cell-derived macrophages were treated with Lca (50, 500, or 5 mg/mL) ( 12 ) in the absence or presence of LPS (100 ng/mL, InvivoGen, USA) ( 13 ) stimulation for 24 h. Cell viability was then determined by cell counting kit 8 (CCK-8) assay (Dojindo Laboratories, CK04, Japan). The CCK-8 reagent (10 μL/well) was directly added into the 96-well plate and incubated for 1.5 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

L-carnitine reduces acute lung injury via mitochondria modulation and inflammation control in pulmonary macrophages

Wu,

He,

Chen

et al. 2023

Braz J Med Biol Res

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Acute lung injury (ALI) or acute respiratory distress syndrome (ARDS) is a critical respiratory syndrome with limited effective interventions. Lung macrophages play a critical role in the pathogenesis of abnormal inflammatory response in the syndrome. Recently, impaired fatty acid oxidation (FAO), one of the key lipid metabolic signalings, was found to participate in the onset and development of various lung diseases, including ALI/ARDS. Lipid/fatty acid contents within mouse lungs were quantified using the Oil Red O staining. The protective effect of FAO activator L-carnitine (Lca, 50, 500, or 5 mg/mL) was evaluated by cell counting kit 8 (CCK-8) assay, real-time quantitative PCR (qPCR), ELISA, immunoblotting, fluorescence imaging, and fluorescence plate reader detection in lipopolysaccharide (LPS) (100 ng/mL)-stimulated THP-1-derived macrophages. The in vivo efficacy of Lca (300 mg/kg) was determined in a 10 mg/kg LPS-induced ALI mouse model. We found for the first time that lipid accumulation in pulmonary macrophages was significantly increased in a classical ALI murine model, which indicated disrupted FAO induced by LPS. Lca showed potent anti-inflammatory and antioxidative effects on THP-1 derived macrophages upon LPS stimulation. Mechanistically, Lca was able to maintain FAO, mitochondrial activity, and ameliorate mitochondrial dynamics. In the LPS-induced ALI mouse model, we further discovered that Lca inhibited neutrophilic inflammation and decreased diffuse damage, which might be due to the preservation of mitochondrial homeostasis. These results broadened our understanding of ALI/ARDS pathogenesis and provided a promising drug candidate for this syndrome.

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Section: Methodsmentioning

confidence: 99%

L-carnitine reduces acute lung injury via mitochondria modulation and inflammation control in pulmonary macrophages

Wu,

He,

Chen

et al. 2023

Braz J Med Biol Res

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“…Changes in the content of these proteins are considered to be of great significance in the physiological functions of the lung epithelium and endothelial barrier [ 8 ]. During ALI, pro-inflammatory cytokines could induce and aggravate barrier dysfunction by disrupting the expression of TJPs and increasing apoptosis [ 9 ]. Pulmonary barrier dysfunction further exacerbates inflammatory cell infiltration, suppresses inflammation, and exacerbates ALI progression.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Vitamin K2 (MK-7) on Acute Lung Injury Induced by Lipopolysaccharide in Mice

Yang,

Wang,

Liu

et al. 2024

CIMB

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Vitamin K2 (MK-7) has been shown to cause significant changes in different physiological processes and diseases, but its role in acute lung injury (ALI) is unclear. Therefore, in this study, we aimed to evaluate the protective effects of VK2 against LPS-induced ALI in mice. The male C57BL/6J mice were randomly divided into six groups (n = 7): the control group, LPS group, negative control group (LPS + Oil), positive control group (LPS + DEX), LPS + VK2 (L) group (VK2, 1.5 mg/kg), and LPS + VK2 (H) group (VK2, 15 mg/kg). Hematoxylin–eosin (HE) staining of lung tissue was performed. Antioxidant superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) activities, and the Ca2+ level in the lung tissue were measured. The effects of VK2 on inflammation, apoptosis, tight junction (TJ) injury, mitochondrial dysfunction, and autophagy were quantitatively assessed using Western blot analysis. Compared with the LPS group, VK2 improved histopathological changes; alleviated inflammation, apoptosis, and TJ injury; increased antioxidant enzyme activity; reduced Ca2+ overload; regulated mitochondrial function; and inhibited lung autophagy. These results indicate that VK2 could improve tight junction protein loss, inflammation, and cell apoptosis in LPS-induced ALI by inhibiting the mitochondrial dysfunction and excessive autophagy, indicating that VK2 plays a beneficial role in ALI and might be a potential therapeutic strategy.

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“…This neurotransmitter is essential for neuron development, attention, learning, and controlling stress reactions. The central nervous system, kidneys, hormones, motivational activities, and cardiovascular system are among its primary functions . The functioning of the brain, feelings, and behavior of humans and animals is dependent on the concentration of dopamine.…”

Section: Introductionmentioning

confidence: 99%

“…The functioning of the brain, feelings, and behavior of humans and animals is dependent on the concentration of dopamine. Parkinson’s disease, schizophrenia, attention deficit hyperactivity disorder, and other diseases are said to be caused by abnormal amounts of dopamine concentrations. − These disorders are a result of damage to the involved neurons during the production of dopamine.…”

Section: Introductionmentioning

confidence: 99%

“…The central nervous system, kidneys, hormones, motivational activities, and cardiovascular system are among its primary functions. 2 The functioning of the brain, feelings, and behavior of humans and animals is dependent on the concentration of dopamine. Parkinson's disease, schizophrenia, attention deficit hyperactivity disorder, and other diseases are said to be caused by abnormal amounts of dopamine concentrations.…”

Section: Introductionmentioning

confidence: 99%

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Dendrimer─Gold Nanocomposite-Based Electrochemical Aptasensor for the Detection of Dopamine

Sipuka,

Olorundare,

Makaluza

et al. 2023

ACS Omega

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Dopamine is an important neurotransmitter and biomarker that plays a vital role in our neurological system and body. Thus, it is important to monitor the concentration levels of dopamine in our bodies. We report an aptamer-based sensor fabricated through an electro-co-deposition of a generation 3 poly(propylene imine) (PPI) dendrimer and gold nanoparticles (AuNPs) on a glassy carbon (GC) electrode by cyclic voltammetry. Through self-assembly, a single-stranded thiolated dopamine aptamer was immobilized on the GC/PPI/AuNPs electrode to prepare an aptasensor. Voltammetry and electrochemical impedance spectroscopy (EIS) were used to characterize the modified electrodes. The readout for the biorecognition event between the aptamer and various dopamine concentrations was attained from square wave voltammetry and EIS. The aptasensor detected dopamine from the range of 10−200 nM, with a limit of detection of 0.26 and 0.011 nM from SWV and EIS, respectively. The aptasensor was selective toward dopamine when different amounts of epinephrine and ascorbic acid were present. The aptasensor was applicable in a more complex matrix of human serum.

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Dopamine D1 receptor agonist alleviates acute lung injury via modulating inflammatory responses in macrophages and barrier function in airway epithelial cells

Cited by 11 publications

References 57 publications

L-carnitine reduces acute lung injury via mitochondria modulation and inflammation control in pulmonary macrophages

L-carnitine reduces acute lung injury via mitochondria modulation and inflammation control in pulmonary macrophages

Protective Effect of Vitamin K2 (MK-7) on Acute Lung Injury Induced by Lipopolysaccharide in Mice

Dendrimer─Gold Nanocomposite-Based Electrochemical Aptasensor for the Detection of Dopamine

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