Dopamine decreases expression of type-1 angiotensin II receptors in renal proximal tubule.

Cheng, Hao; Becker, Bryan N.; Harris, Raymond C.

doi:10.1172/jci118729

Cited by 80 publications

(68 citation statements)

References 50 publications

(50 reference statements)

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Section: Discussionmentioning

confidence: 99%

“…15,16 Dopamine, via D 1 -like receptors, has also been reported to decrease the AT 1 receptor mRNA and protein, as well as angiotensin II binding sites in renal proximal tubules. 19,42 The effect of angiotensin II on D 1 -like receptors has not been reported.In WKY RPT cells, stimulation of the AT 1 receptor with angiotensin II for 2 different periods (short-term or longterm) yields opposite results; short-term stimulation decreases cell surface D 1 receptor expression, whereas longterm stimulation increases whole cell D 1 receptor expression. It is unlikely that the change in the short-term could account for the long-term result.…”

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confidence: 99%

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Rat Strain Effects of AT ₁ Receptor Activation on D ₁ Dopamine Receptors in Immortalized Renal Proximal Tubule Cells

et al. 2005

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Abstract-The dopaminergic and renin-angiotensin systems regulate blood pressure, in part, by affecting sodium transport in renal proximal tubules (RPTs). We have reported that activation of a D 1 -like receptor decreases AT 1 receptor expression in the mouse kidney and in immortalized RPT cells from Wistar-Kyoto (WKY) rats. The current studies were designed to test the hypothesis that activation of the AT 1 receptor can also regulate the D 1 receptor in RPT cells, and this regulation is aberrant in spontaneously hypertensive rats (SHRs Key Words: dopamine Ⅲ kidney Ⅲ receptors, angiotensin II Ⅲ rats, spontaneously hypertensive A ngiotensin II and dopamine are important regulators of sodium and water transport across the renal proximal tubule (RPT). [1][2][3][4][5][6][7][8] Angiotensin II receptor subtypes (AT 1 , AT 2 , and AT 4 ) are expressed in brush border and basolateral membranes of RPTs. [1][2][3][4][5] The activation of AT 1 receptors by low concentrations of angiotensin II (picomolar) causes an increase in sodium reabsorption in RPTs. 1,3,6 All the dopamine receptor subtypes, D 1 -like (D 1 and D 5 ) and D 2 -like (D 2 , D 3 , and D 4 ) are also expressed in brush border and basolateral membranes of RPTs. [7][8][9][10] In contrast to the stimulatory effect of AT 1 receptors on renal sodium transport, activation of D 1 and D 3 receptors decreases renal sodium reabsorption. 7,8,[11][12][13] Several reports have shown an interaction between the dopamine and renin-angiotensin system. Intrarenally produced angiotensin II opposes the natriuretic action of the D1-like dopamine receptor agonist, fenoldopam, in rats. 14 D 2 -like receptor agonists also antagonize the stimulatory effect of angiotensin II, acting via AT 1 receptors, on renal proximal tubular luminal sodium transport. 15,16 When angiotensin II generation is inhibited or AT 1 receptors are blocked, the natriuretic effect of dopaminergic drugs is enhanced. 17 The AT 1 receptor mediates the anti-natriuretic effect of angiotensin II, whereas the D 1 dopamine receptor is responsible for Ϸ80% of D 1 -like receptor activity in RPTs. 18 We have reported that in RPT cells of Wistar-Kyoto (WKY) Methods Cell CultureImmortalized RPT cells from 4-to 8-week-old WKY and SHRs were cultured at 37°C in 95% air/5% CO 2 atmosphere in DMEM/F-12 culture media, as previously described. [21][22][23][24][25][26] The cells (80% confluence) were extracted in ice-cold lysis buffer (phosphate-buffered saline with 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mmol/L EDTA, 1 mmol/L EGTA, 1 mmol/L PMSF, 10 g/mL aprotinin, and 10 g/mL leupeptin), sonicated, kept on ice for 1 hour, and centrifuged at 16 000g for 30 minutes. The supernatants were stored at Ϫ70°C until use for immunoblotting and/or immunoprecipitation. ImmunoblottingThe antibodies are polyclonal purified antipeptides. The amino acid sequence for the immunogenic peptide (rabbit anti-human AT 1 receptor antibody) is QDDCPKAGRHC, amino acids 15 to 24 of the AT 1 receptor. The specificity of this antibody to the AT 1 ...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Rat Strain Effects of AT ₁ Receptor Activation on D ₁ Dopamine Receptors in Immortalized Renal Proximal Tubule Cells

et al. 2005

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“…The receptors for Angiontensin II are located in the apical and basolateral membrane of the proximal tubules. 17 DA, through D 1 receptors, diminishes the expression of AT 1 receptors in the proximal tubule; this effect is mediated by an increase in the intracellular cAMP concentration. 17…”

Section: Angiotensin IImentioning

confidence: 98%

“…16 Proximal tubules produce DA by means of L-DOPA uptake from tubular filtrate through a Na + dependent transportation mechanism, which is further decarboxylated into DA by AADC (Laromatic amino acid decarboxylase). 17 Since DA urine levels are much higher than levels circulating in plasma, synthesis in proximal tubules is said to be the main renal DA source, because AADC inhibition reduces DA excretion through urine. 17 L-DOPA uptake through distal tubules and DA production takes place by means of ␣ 2 -adrenergic receptors.…”

Section: Role Of Dopamine In Kidney Functionmentioning

confidence: 99%

“…17 Since DA urine levels are much higher than levels circulating in plasma, synthesis in proximal tubules is said to be the main renal DA source, because AADC inhibition reduces DA excretion through urine. 17 L-DOPA uptake through distal tubules and DA production takes place by means of ␣ 2 -adrenergic receptors. 18 DA capability of producing kidney natriuresis and diuresis is mainly the result of the inhibition of two water and sodium transportation complexes in proximal nephron.…”

Section: Role Of Dopamine In Kidney Functionmentioning

confidence: 99%

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Dopamine, hypertension and obesity

et al. 2002

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Dopamine and Renal Function and Blood Pressure Regulation

Armando

Villar

José

2011

Comprehensive Physiology

108

215

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Dopamine is an important regulator of systemic blood pressure via multiple mechanisms. It affects fluid and electrolyte balance by its actions on renal hemodynamics and epithelial ion and water transport and by regulation of hormones and humoral agents. The kidney synthesizes dopamine from circulating or filtered L-DOPA independently from innervation. The major determinants of the renal tubular synthesis/release of dopamine are probably sodium intake and intracellular sodium. Dopamine exerts its actions via two families of cell surface receptors, D1-like receptors comprising D1R and D5R, and D2-like receptors comprising D2R, D3R, and D4R, and by interactions with other G protein-coupled receptors. D1-like receptors are linked to vasodilation, while the effect of D2-like receptors on the vasculature is variable and probably dependent upon the state of nerve activity. Dopamine secreted into the tubular lumen acts mainly via D1-like receptors in an autocrine/paracrine manner to regulate ion transport in the proximal and distal nephron. These effects are mediated mainly by tubular mechanisms and augmented by hemodynamic mechanisms. The natriuretic effect of D1-like receptors is caused by inhibition of ion transport in the apical and basolateral membranes. D2-like receptors participate in the inhibition of ion transport during conditions of euvolemia and moderate volume expansion. Dopamine also controls ion transport and blood pressure by regulating the production of reactive oxygen species and the inflammatory response. Essential hypertension is associated with abnormalities in dopamine production, receptor number, and/or posttranslational modification.

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Dopamine decreases expression of type-1 angiotensin II receptors in renal proximal tubule.

Cited by 80 publications

References 50 publications

Rat Strain Effects of AT ₁ Receptor Activation on D ₁ Dopamine Receptors in Immortalized Renal Proximal Tubule Cells

Rat Strain Effects of AT ₁ Receptor Activation on D ₁ Dopamine Receptors in Immortalized Renal Proximal Tubule Cells

Dopamine, hypertension and obesity

Dopamine and Renal Function and Blood Pressure Regulation

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Dopamine decreases expression of type-1 angiotensin II receptors in renal proximal tubule.

Cited by 80 publications

References 50 publications

Rat Strain Effects of AT 1 Receptor Activation on D 1 Dopamine Receptors in Immortalized Renal Proximal Tubule Cells

Rat Strain Effects of AT 1 Receptor Activation on D 1 Dopamine Receptors in Immortalized Renal Proximal Tubule Cells

Dopamine, hypertension and obesity

Dopamine and Renal Function and Blood Pressure Regulation

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Rat Strain Effects of AT ₁ Receptor Activation on D ₁ Dopamine Receptors in Immortalized Renal Proximal Tubule Cells

Rat Strain Effects of AT ₁ Receptor Activation on D ₁ Dopamine Receptors in Immortalized Renal Proximal Tubule Cells