Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125

Abstract

Dopmine functionalized mesoporous onion-like silica (DPMS) was synthesized via a biomimetic coating, and lipase Candida sp. 99-125 (LCS) was immobilized in DPMS (LCS@DPMS) by physical adsorption in this study. The DPMS was characterized by SEM, TEM, BET and FT-IR, and it was shown that the DPMS had clear multishell structures with large surface area of 419 m2/g. The activity, pH stability, thermal stability, storage stability, and reusability of the LCS@DPMS were investigated in detail. The stabilities of LCS@DPMS were improved significantly compared to the free lipase and LCS@MS (LCS immobilized in unfunctionalized mesoporous onion-like silica by physical adsorption). All the results indicated that the DPMS had high efficiency and improved stability for lipase immobilization.

Facile functionalized mesoporous silica using biomimetic method as new matrix for preparation of shape‐stabilized phase‐change material with improved enthalpy

“…32 After modification with dopamine, poly- Figure 5C and 5D, new chemical bonds of C═O (287.1 eV) and C═C (284.2 eV) could be found in Figure 5D, which represented the C 1s of poly-dopamine on the surface of DPMS. Furthermore, the three sources of the N element were -NH (399.7 eV), -NH 2 (401.6 eV), and -N = (398.9 eV), 26,27 which indicated that dopamine attached onto SBA-15 successfully. 33 According to Figure 5F, the N element could be detected easily in DPMS.…”

“…33 According to Figure 5F, the N element could be detected easily in DPMS. Furthermore, the three sources of the N element were -NH (399.7 eV), -NH 2 (401.6 eV), and -N = (398.9 eV), 26,27 which indicated that dopamine attached onto SBA-15 successfully. Figure 6 exhibits the XPS test curves of DPMS and SBA-15.…”

“…18 However, the surface modification of inorganic material usually required refluxing with organic solvent, which not only had the disadvantages of high energy and time consuming but also could easily leave toxic solvents in the pores of inorganic material. 26,27 Inspired by the above research results, our team synthesized a new kind of ss-PCM (PEG/Dop-SF), which was prepared using dopamine-functionalized silica fume (SF) as the support of PEG, and the study results showed that dopamine modification could improve the phase-change behavior of PEG, and then the enthalpy of PEG/Dop-SF was increased. Therefore, it is necessary to find a simple and environmentally friendly way to modify the inorganic materials for the preparation of ss-PCMs.…”

“…24,25 Dopamine is a widely used chemical substance with the catechol functional groups that can achieve superbonding on the surface of a variety of different substrates. 26,27 Inspired by the above research results, our team synthesized a new kind of ss-PCM (PEG/Dop-SF), which was prepared using dopamine-functionalized silica fume (SF) as the support of PEG, and the study results showed that dopamine modification could improve the phase-change behavior of PEG, and then the enthalpy of PEG/Dop-SF was increased. 21 However, when the SF was applied as the supporter of organic PCMs, it had the shortcomings of small pore volume and pore size, which could bring about the blocking of the inner channels of SF while it was modified with dopamine, and then the loading amount of the organic PCMs decreased.…”

Synthesis of novel mesoporous materials as high-performance adsorbents

“…56 Lipase generally shows a severe decrease in catalytic activity after immobilization which rises from the increased mass-transfer limitations between the enzyme and substrate, the fractional blocking of enzyme active sites or alternation of native enzyme structure during the immobilization process. [110][111][112][113] Interfacial activation concept has developed as an elegant approach to enhance the activity of immobilized lipase over the recent years. 114 It has been well-documented that the lipase molecule possesses a flexible configuration in which the active site is covered by a hydrophobic and movable region of the lipase molecule, called the "lid".…”