Dopamine-mediated calcium channel regulation in synaptic suppression in L. stagnalis interneurons

Dong, Nancy; Lee, David W. K.; Sun, Hong‐Shuo; Feng, Zhong‐Ping

doi:10.1080/19336950.2018.1457897

Cited by 12 publications

(11 citation statements)

References 102 publications

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“…Likewise, dopamine, acting at G protein-coupled receptors, produced only voltage-independent inhibition of Ca 2ϩ channels in Aplysia neurons (37), despite its ability to produce voltage-dependent inhibition of I Ca in vertebrates. More recently, however, evidence has emerged that dopamine mediates inhibition at a Lymnaea synapse, at least partially via voltage-dependent inhibition of I Ca (27). Our findings here that invertebrate VGCCs are indeed capable of undergoing G protein-mediated voltagedependent inhibition provide further compelling evidence that this type of neuronal inhibition is conserved between vertebrates and at least some invertebrates.…”

Section: Invertebrate Voltage-gated Ca 2؉ Channels Are Capable Of Undsupporting

confidence: 60%

“…The slowing of activation kinetics and the depolarizing shift in the voltage dependence of channel activation (with no effect on channel inactivation) produced by HX531 are both characteristic of the voltage-dependent inhibition of VGCCs in vertebrate neurons. Voltage-dependent inhibition can be relieved by a large depolarizing prepulse, known to cause the release of inhibitory G␤␥ proteins from the channel (23)(24)(25)(26)(27). To determine whether HX531 produced voltage-dependent inhibition in VF neurons, the cells were treated overnight with either 1 M or 500 nM HX531 or the equivalent concentration of DMSO (0.01%).…”

Section: Hx531 and Le540 Produce Voltage-dependent Inhibition Of Vgccsmentioning

confidence: 99%

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Retinoid receptor-based signaling plays a role in voltage-dependent inhibition of invertebrate voltage-gated Ca2+ channels

Hoog

Lukewich

Spencer

2019

Journal of Biological Chemistry

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Edited by Mike Shipston The retinoic acid receptor (RAR) and retinoid X receptor (RXR) mediate the cellular effects of retinoids (derivatives of vitamin A). Both RAR and RXR signaling events are implicated in hippocampal synaptic plasticity. Furthermore, retinoids can interact with calcium signaling during homeostatic plasticity. We recently provided evidence that retinoids attenuate calcium current (I Ca) through neuronal voltage-gated calcium channels (VGCCs). We now examined the possibility that constitutive activity of neuronal RXR and/or RAR alters calcium influx via the VGCCs. We found that in neurons of the mollusk Lymnaea stagnalis, two different RXR antagonists (PA452 and HX531) had independent and opposing effects on I Ca that were also time-dependent; whereas the RXR pan-antagonist PA452 enhanced I Ca , HX531 reduced I Ca. Interestingly, this effect of HX531 occurred through voltage-dependent inhibition of VGCCs, a phenomenon known to influence neurotransmitter release from neurons. This inhibition appeared to be independent of G proteins and was largely restricted to Ca v 2 Ca 2؉ channels. Of note, an RAR pan-antagonist, LE540, also inhibited I Ca but produced G protein-dependent, voltage-dependent inhibition of VGCCs. These findings provide evidence that retinoid receptors interact with G proteins in neurons and suggest mechanisms by which retinoids might affect synaptic calcium signaling. Voltage-gated Ca 2ϩ channels (VGCCs) 2 play a prominent role in the functioning of both vertebrate and invertebrate nervous systems. High voltage-activated VGCCs include both Ca v 1 (L-type) and Ca v 2 (non-L-type) channels. In the nervous system, Ca v 1 channels are generally involved in the regulation of gene transcription, whereas Ca v 2 channels play an important role in neurotransmitter release (1). Importantly, high voltageactivated channels are modulated by diverse signaling pathways and can therefore contribute to dynamic changes in cell function.

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Section: Invertebrate Voltage-gated Ca 2؉ Channels Are Capable Of Undsupporting

confidence: 60%

Section: Hx531 and Le540 Produce Voltage-dependent Inhibition Of Vgccsmentioning

confidence: 99%

Retinoid receptor-based signaling plays a role in voltage-dependent inhibition of invertebrate voltage-gated Ca2+ channels

Hoog

Lukewich

Spencer

2019

Journal of Biological Chemistry

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“…Finally, for the first time, we systematically identified transcripts that putatively encode 43 subtypes of Na + , K + , Ca 2+ , Cland cation ion channels and ionotropic neurotransmitter receptors (Figure 8-14, Table S15-23), greatly expanding our understanding of the molecular determinants of neural excitability and transmission in the L. stagnalis CNS. As the L. stagnalis CNS has been widely employed to study ion channels and/or ionotropic neurotransmitter receptors (Spafford, Chen, et al 2003;Lu and Feng 2011;Dawson et al 2014;Dong, Lee, et al 2018), we examined the protein sequence similarity of each identified L. stagnalis channel/receptor transcripts with its homolog in mouse, X. tropicalis, zebrafish, fruit fly and C. elegans.…”

Section: Discussionmentioning

confidence: 99%

Ion channel profiling of the Lymnaea stagnalis ganglia via transcriptome analysis

Dong

Bandura

Zhang

et al. 2020

Preprint

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Background. The pond snail Lymnaea stagnalis (L. stagnalis) has been widely used as a model organism in neurobiology, ecotoxicology, and parasitology due to the relative simplicity of its CNS. However, its usefulness is restricted by a limited availability of transcriptome data. While sequence information for the L. stagnalis CNS transcripts has been obtained from EST library and a de novo RNA-seq assembly, the quality of these assemblies is limited by a combination of low coverage of EST libraries, the fragmented nature of de novo assemblies, and lack of reference genome. Results. In this study, taking advantage of the recent availability of the L. stagnalis reference genome, we generated an RNA-seq library from the adult L. stagnalis CNS, using a combination of genome-guided and de novo assembly programs to identify 17,832 protein-coding L. stagnalis transcripts. We combined our library with existing resources to produce a transcript set with greater sequence length, completeness, and diversity than previously available ones. Using our assembly and functional domain analysis, we profiled L. stagnalis CNS transcripts encoding ion channels and ionotropic receptors, which are key proteins for CNS function, and compared their sequences to other vertebrate and invertebrate model organisms. Interestingly, L. stagnalis transcripts encoding numerous putative Ca2+ channels showed the most sequence similarity to those of mouse, zebrafish, Xenopus tropicalis, fruit fly, and C. elegans, suggesting that many calcium channel-related signaling pathways may be evolutionarily conserved. Conclusions. Our study provides the most thorough characterization to date of the L. stagnalis transcriptome and provides insights into differences between vertebrates and invertebrates in CNS transcript diversity, according to function and protein class. Furthermore, this study is, to the best of our knowledge, the first to provide a complete characterization of the ion channels of a single species, opening new avenues for future research on fundamental neurobiological processes.

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“…Finally, for the rst time, we systematically identi ed transcripts that putatively encode 43 subtypes of Na + , K + , Ca 2+ , Cl -, cation, and TRP ion channels and ionotropic neurotransmitter receptors (Figure 8-14, Table S15-23), greatly expanding our understanding of the molecular determinants of neural excitability and transmission in the L. stagnalis CNS. As the L. stagnalis CNS has been widely employed to study ion channels and/or ionotropic neurotransmitter receptors [38,[75][76][77], we examined the protein sequence similarity of each identi ed L. stagnalis channel/receptor transcripts with its homolog in M. musculus, X. tropicalis, D. rerio, D. melanogaster and C. elegans.…”

Section: Discussionmentioning

confidence: 99%

Ion channel profiling of the Lymnaea stagnalis ganglia via transcriptome analysis

Dong

Bandura

Zhang

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background. The pond snail Lymnaea stagnalis (L. stagnalis) has been widely used as a model organism in neurobiology, ecotoxicology, and parasitology due to the relative simplicity of its central nervous system (CNS). However, its usefulness is restricted by a limited availability of transcriptome data. While sequence information for the L. stagnalis CNS transcripts has been obtained from EST library and a de novo RNA-seq assembly, the quality of these assemblies is limited by a combination of low coverage of EST libraries, the fragmented nature of de novo assemblies, and lack of reference genome. Results. In this study, taking advantage of the recent availability of a preliminary L. stagnalis genome, we generated an RNA-seq library from the adult L. stagnalis CNS, using a combination of genome-guided and de novo assembly programs to identify 17,832 protein-coding L. stagnalis transcripts. We combined our library with existing resources to produce a transcript set with greater sequence length, completeness, and diversity than previously available ones. Using our assembly and functional domain analysis, we profiled L. stagnalis CNS transcripts encoding ion channels and ionotropic receptors, which are key proteins for CNS function, and compared their sequences to other vertebrate and invertebrate model organisms. Interestingly, L. stagnalis transcripts encoding numerous putative Ca2+ channels showed the most sequence similarity to those of Mus musculus, Danio rerio, Xenopus tropicalis, Drosophila melanogaster, and Caenorhabditis elegans, suggesting that many calcium channel-related signaling pathways may be evolutionarily conserved. Conclusions. Our study provides the most thorough characterization to date of the L. stagnalis transcriptome and provides insights into differences between vertebrates and invertebrates in CNS transcript diversity, according to function and protein class. Furthermore, this study provides a complete characterization of the ion channels of Lymnaea stagnalis, opening new avenues for future research on fundamental neurobiological processes in this model system.

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Dopamine-mediated calcium channel regulation in synaptic suppression in L. stagnalis interneurons

Cited by 12 publications

References 102 publications

Retinoid receptor-based signaling plays a role in voltage-dependent inhibition of invertebrate voltage-gated Ca2+ channels

Retinoid receptor-based signaling plays a role in voltage-dependent inhibition of invertebrate voltage-gated Ca2+ channels

Ion channel profiling of the Lymnaea stagnalis ganglia via transcriptome analysis

Ion channel profiling of the Lymnaea stagnalis ganglia via transcriptome analysis

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