Dopamine receptor D4 (DRD4) negatively regulates UCP1- and ATP-dependent thermogenesis in 3T3-L1 adipocytes and C2C12 muscle cells

Haddish, Kiros; Yun, Jong Won

doi:10.1007/s00424-023-02816-w

Cited by 5 publications

(1 citation statement)

References 48 publications

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“…SKF38393, a DRD1/5 agonist, promoted mitochondrial transport in cultured hippocampal neurons [ 44 ]. Our recent studies also indicated that silencing of DRD4 increased the mitochondrial biogenesis effectors [ 45 ], while the knockdown of DRD5 exhibited downregulation of these effectors including the OXPHOS complexes in 3T3-L1 and C2C12 cells [ 46 ], suggesting their opposite effect on mitochondrial biogenesis. However, further research is required to investigate the effect of ECH on OXPHOS complexes, specifically complex V (ATP synthase).…”

Section: Discussionmentioning

confidence: 99%

Echinacoside Induces UCP1- and ATP-Dependent Thermogenesis in Beige Adipocytes via the Activation of Dopaminergic Receptors

Haddish

Yun

2023

J. Microbiol. Biotechnol.

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Echinacoside (ECH) is a naturally occurring phenylethanoid glycoside, isolated from Echinacea angustifolia , and this study aimed to analyze its effect on thermogenesis and its interaction with dopaminergic receptors 1 and 5 (DRD1 and DRD5) in 3T3-L1 white adipocytes and mice models. We employed RT-PCR, immunoblot, immunofluorescence, a staining method, and an assay kit to determine its impact. ECH showed a substantial increase in browning signals in vitro and a decrease in adipogenic signals in vivo. Additionally, analysis of the iWAT showed that the key genes involved in beiging, mitochondrial biogenesis, and ATP-dependent thermogenesis were upregulated while adipogenesis and lipogenesis genes were downregulated. OXPHOS complexes, Ca 2+ signaling proteins as well as intracellular Ca 2+ levels were also upregulated in 3T3-L1 adipocytes following ECH treatment. This was collectively explained by mechanistic studies which showed that ECH mediated the beiging process via the DRD1/5-cAMP-PKA and subsequent downstream molecules, whereas it co-mediated the α1-AR-signaling thermogenesis via the DRD1/5/SERCA2b/RyR2/CKmt pathway in 3T3-L1 adipocytes. Animal experiments revealed that there was a 12.28% reduction in body weight gain after the ECH treatment for six weeks. The effects of ECH treatment on adipose tissue can offer more insights into the treatment of obesity and metabolic syndrome.

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Section: Discussionmentioning

confidence: 99%

Echinacoside Induces UCP1- and ATP-Dependent Thermogenesis in Beige Adipocytes via the Activation of Dopaminergic Receptors

Haddish

Yun

2023

J. Microbiol. Biotechnol.

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Dopamine in the regulation of glucose and lipid metabolism: a narrative review

Li,

Zheng,

Wang

et al. 2024

Obesity

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ObjectiveOwing to the global obesity epidemic, understanding the regulatory mechanisms of glucose and lipid metabolism has become increasingly important. The dopaminergic system, including dopamine, dopamine receptors, dopamine transporters, and other components, is involved in numerous physiological and pathological processes. However, the mechanism of action of the dopaminergic system in glucose and lipid metabolism is poorly understood. In this review, we examine the role of the dopaminergic system in glucose and lipid metabolism.ResultsThe dopaminergic system regulates glucose and lipid metabolism through several mechanisms. It regulates various activities at the central level, including appetite control and decision‐making, which contribute to regulating body weight and energy metabolism. In the pituitary gland, dopamine inhibits prolactin production and promotes insulin secretion through dopamine receptor 2. Furthermore, it can influence various physiological components in the peripheral system, such as pancreatic β cells, glucagon‐like peptide‐1, adipocytes, hepatocytes, and muscle, by regulating insulin and glucagon secretion, glucose uptake and use, and fatty acid metabolism.ConclusionsThe role of dopamine in regulating glucose and lipid metabolism has significant implications for the physiology and pathogenesis of disease. The potential therapeutic value of dopamine lies in its effects on metabolic disorders.

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Homotaurine exhibits contrasting effects of DRD1-mediated thermogenesis-related regulators in C2C12 myoblasts and 3T3−L1 white adipocytes

Haddish,

Yun

2024

Biotechnol Bioproc E

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Dopamine receptor D4 (DRD4) negatively regulates UCP1- and ATP-dependent thermogenesis in 3T3-L1 adipocytes and C2C12 muscle cells

Cited by 5 publications

References 48 publications

Echinacoside Induces UCP1- and ATP-Dependent Thermogenesis in Beige Adipocytes via the Activation of Dopaminergic Receptors

Echinacoside Induces UCP1- and ATP-Dependent Thermogenesis in Beige Adipocytes via the Activation of Dopaminergic Receptors

Dopamine in the regulation of glucose and lipid metabolism: a narrative review

Homotaurine exhibits contrasting effects of DRD1-mediated thermogenesis-related regulators in C2C12 myoblasts and 3T3−L1 white adipocytes

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