Dopaminergic differentiation of stem cells from human deciduous teeth and their therapeutic benefits for Parkinsonian rats

Fujii, Hiromi; Matsubara, Kazuki; Saito, Kiyoshi; Ito, Mikako; Ohno, Kinji; Ueda, Minoru; Yamamoto, Akihito

doi:10.1016/j.brainres.2015.04.001

Cited by 95 publications

(116 citation statements)

References 54 publications

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“…Furthermore, SHED-CM suppressed 6-hydroxydopamine-induced cell death and enhanced the neurite growth of cerebellar granule neurons. The trophic activity of SHED improved neurological deficits of Parkinsonian rats [60]. In this study, we showed that SHED-CM protected primary cerebral neurons against glutamate neurotoxicity.…”

Section: Discussionsupporting

confidence: 54%

Conditioned medium from the stem cells of human dental pulp improves cognitive function in a mouse model of Alzheimer’s disease

Mita

Furukawa‐Hibi

Takeuchi

et al. 2015

Behavioural Brain Research

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139

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Section: Discussionsupporting

confidence: 54%

Conditioned medium from the stem cells of human dental pulp improves cognitive function in a mouse model of Alzheimer’s disease

Mita

Furukawa‐Hibi

Takeuchi

et al. 2015

Behavioural Brain Research

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139

132

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“…Predifferentiated SHED-derived neurospheres were applied into the striatum of parkinsonian rats and significant improvement in behavioral impairment as compared to the implantation of control undifferentiated SHED was reported [36]. Similar results were achieved after inducing neural maturation of SHED into dopaminergic neuron-like cells and transplantation in parkinsonian rats [150]. Moreover, transplantation of neural-induced SHED in a rat Spinal Cord Injury (SCI) model led to complete recovery of hindlimb motor function [148].…”

Section: Differentiation Potential and Paracrine Activity Of Dentamentioning

confidence: 98%

Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Bakopoulou

About

2016

Stem Cells International

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Dental Mesenchymal Stem Cells (MSCs), including Dental Pulp Stem Cells (DPSCs), Stem Cells from Human Exfoliated Deciduous teeth (SHED), and Stem Cells From Apical Papilla (SCAP), have been extensively studied using highly sophisticated in vitro and in vivo systems, yielding substantially improved understanding of their intriguing biological properties. Their capacity to reconstitute various dental and nondental tissues and the inherent angiogenic, neurogenic, and immunomodulatory properties of their secretome have been a subject of meticulous and costly research by various groups over the past decade. Key milestone achievements have exemplified their clinical utility in Regenerative Dentistry, as surrogate therapeutic modules for conventional biomaterial-based approaches, offering regeneration of damaged oral tissues instead of simply “filling the gaps.” Thus, the essential next step to validate these immense advances is the implementation of well-designed clinical trials paving the way for exploiting these fascinating research achievements for patient well-being: the ultimate aim of this ground breaking technology. This review paper presents a concise overview of the major biological properties of the human dental MSCs, critical for the translational pathway “from bench to clinic.”

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“…These cells originate from the peripheral nerve‐associated glia and in contrast to the MSC‐like cells derived from other mesodermal tissues have unique neurogenic properties . Moreover, SHEDs were efficiently differentiated into dopaminergic neuron‐like cells and Schwann cells . Our previous in vitro studies revealed that EVs derived from SHEDs can suppress 6‐hydroxydopamine (6‐OHDA)‐induced apoptosis of human dopaminergic neurons .…”

Section: Introductionmentioning

confidence: 99%

Intranasal Administration of Extracellular Vesicles Derived from Human Teeth Stem Cells Improves Motor Symptoms and Normalizes Tyrosine Hydroxylase Expression in the Substantia Nigra and Striatum of the 6-Hydroxydopamine-Treated Rats

Narbute

Piļipenko

Pupure

et al. 2019

Stem Cells Translational Medicine

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Parkinson's disease (PD) is the second most common neurodegenerative disorder affecting millions of people worldwide. At present, there is no effective cure for PD; treatments are symptomatic and do not halt progression of neurodegeneration. Extracellular vesicles (EVs) can cross the blood–brain barrier and represent promising alternative to the classical treatment strategies. In the present study, we examined therapeutic effects of intranasal administration of EVs derived from human exfoliated deciduous teeth stem cells (SHEDs) on unilateral 6‐hydroxydopamine (6‐OHDA) medial forebrain bundle (MFB) rat model of PD. CatWalk gait tests revealed that EVs effectively suppressed 6‐OHDA‐induced gait impairments. All tested gait parameters (stand, stride length, step cycle, and duty cycle) were significantly improved in EV‐treated animals when compared with 6‐OHDA‐lesion group rats. Furthermore, EVs slowed down numbers of 6‐OHDA‐induced contralateral rotations in apomorphine test. Improvements in motor function correlated with normalization of tyrosine hydroxylase expression in the striatum and substantia nigra. In conclusion, we demonstrated, for the first time, the therapeutic efficacy of intranasal administration of EVs derived from SHEDs in a rat model of PD induced by 6‐OHDA intra‐MFB lesion. Our findings could be potentially exploited for the development of new treatment strategies against PD.

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Dopaminergic differentiation of stem cells from human deciduous teeth and their therapeutic benefits for Parkinsonian rats

Cited by 95 publications

References 54 publications

Conditioned medium from the stem cells of human dental pulp improves cognitive function in a mouse model of Alzheimer’s disease

Conditioned medium from the stem cells of human dental pulp improves cognitive function in a mouse model of Alzheimer’s disease

Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Intranasal Administration of Extracellular Vesicles Derived from Human Teeth Stem Cells Improves Motor Symptoms and Normalizes Tyrosine Hydroxylase Expression in the Substantia Nigra and Striatum of the 6-Hydroxydopamine-Treated Rats

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