Doped diamond-like carbon coatings for surgical instruments reduce protein and prion-amyloid biofouling and improve subsequent cleaning

Secker, T.J.; Hervé, R.; Zhao, Qi; Borisenko, Konstantin B.; Abel, E.W.; Keevil, C. W.

doi:10.1080/08927014.2012.698387

Cited by 26 publications

(18 citation statements)

References 25 publications

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“…Since transmission of prion infectivity from surrogate surgical SS surfaces has been demonstrated in both laboratory animals and cell culture systems (Flechsig et al 2001;Yan et al 2004;Edgeworth et al 2009), a significant amount of research and development has been aimed at producing novel techniques for the removal and, ideally, the destruction of infectious prions (McDonnell & Burke 2003;Dickinson et al 2009;Secker, Hervé, Zhao et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…A large number of novel, specialised chemical agents and protocols have been developed to target prions Lemmer et al 2008;Paspaltsis et al 2009;Secker, Hervé, Zhao et al 2012;Ding et al 2013). However, most have shown discrepancies in their efficacies, are too difficult/ expensive to implement into current SSD procedures and/or could potentially be damaging to instruments and washers/disinfectors.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of humidity retention bags for the reduced adsorption and improved cleaning of tissue proteins including prion-associated amyloid to surgical stainless steel surfaces

et al. 2015

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Increasing drying time adversely affects attachment of tissue proteins and prion-associated amyloid to surgical stainless steel, and reduces the efficacy of commercial cleaning chemistries. This study tested the efficacy of commercial humidity retention bags to reduce biofouling on surgical stainless steel and to improve subsequent cleaning. Surgical stainless steel surfaces were contaminated with ME7-infected brain homogenates and left to dry for 15 to 1,440 min either in air, in dry polythene bags or within humidity retention bags. Residual contamination pre/post cleaning was analysed using Thioflavin T/SYPRO Ruby dual staining and microscope analysis. An increase in biofouling was observed with increased drying time in air or in sealed dry bags. Humidity retention bags kept both protein and prion-associated amyloid minimal across the drying times both pre- and post-cleaning. Therefore, humidity bags demonstrate a cheap, easy to implement solution to improve surgical instrument reprocessing and to potentially reduce associated hospital acquired infections.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficacy of humidity retention bags for the reduced adsorption and improved cleaning of tissue proteins including prion-associated amyloid to surgical stainless steel surfaces

et al. 2015

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“…In addition, DLC has previously been shown to resist microbial and protein attachment [22] which indicates that ta-C also could possess similar antifouling properties. On the other hand, adsorption of bovine albumin serum (BSA) is suggested to be favored on ta-C when compared to other types of DLC with higher hydrogen content and lower sp 3 fraction [23].…”

Section: Introductionmentioning

confidence: 98%

Electrochemical detection of hydrogen peroxide on platinum-containing tetrahedral amorphous carbon sensors and evaluation of their biofouling properties

Tujunen

Kaivosoja

Protopopova

et al. 2015

Materials Science and Engineering: C

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Hydrogen peroxide is the product of various enzymatic reactions, and is thus typically utilized as the analyte in biosensors. However, its detection with conventional materials, such as noble metals or glassy carbon, is often hindered by slow kinetics and biofouling of the electrode. In this study electrochemical properties and suitability to peroxide detection as well as ability to resist biofouling of Pt-doped ta-C samples were evaluated. Pure ta-C and pure Pt were used as references. According to the results presented here it is proposed that combining ta-C with Pt results in good electrocatalytic activity towards H2O2 oxidation with better tolerance towards aqueous environment mimicking physiological conditions compared to pure Pt. In biofouling experiments, however, both the hybrid material and Pt were almost completely blocked after immersion in protein-containing solutions and did not produce any peaks for ferrocenemethanol oxidation or reduction. On the contrary, it was still possible to obtain clear peaks for H2O2 oxidation with them after similar treatment. Moreover, quartz crystal microbalance experiment showed less protein adsorption on the hybrid sample compared to Pt which is also supported by the electrochemical biofouling experiments for H2O2 detection.

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“…Diamond-like carbon coatings can potentially be used as antifouling surfaces against microbial and protein attachment [7]. These materials are very stable and have a good biocompatibility [8,9,10] and they are able to resist bacterial adhesion [11].…”

mentioning

confidence: 99%

Glutamate detection by amino functionalized tetrahedral amorphous carbon surfaces

Kaivosoja

Tujunen

Jokinen

et al. 2015

Talanta

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In this paper, a novel amperometric glutamate biosensor with glutamate oxidase (GlOx) immobilized directly on NH2 functionalized, platinum doped tetrahedral amorphous carbon (ta-C) film, has been successfully developed. First, we demonstrate that direct GlOx immobilization is more effective on amino-groups than on carboxyl-or hydroxyl-groups. Second, we show that anodizing and plasma treatments increase the amount of nitrogen and the proportion of protonated 2 amino groups relative to amino groups on the aminosilane coating, which subsequently results in an increased amount of active GlOx on the surface. This effect, however, is found to be unstable due to unstable electrostatic interactions between GlOx and NH3 + . We demonstrate the detection of glutamate in the concentration range of 10 µM−1mM using the NH2 functionalized Pt doped ta-C surface. The biosensor showed high sensitivity (2.9 nA μM -1 cm -2 ), low detection limit (10 μM) and good storage stability. The electrode response to glutamate was linear in the concentrations ranging from10 µM to 500 µM. In conclusion, the study shows that GlOx immobilization is most effective on aminosilane treated ta-C surface without any pre-treatments and the fabricated sensor structure is able to detect glutamate in the micromolar range.

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Doped diamond-like carbon coatings for surgical instruments reduce protein and prion-amyloid biofouling and improve subsequent cleaning

Cited by 26 publications

References 25 publications

Efficacy of humidity retention bags for the reduced adsorption and improved cleaning of tissue proteins including prion-associated amyloid to surgical stainless steel surfaces

Efficacy of humidity retention bags for the reduced adsorption and improved cleaning of tissue proteins including prion-associated amyloid to surgical stainless steel surfaces

Electrochemical detection of hydrogen peroxide on platinum-containing tetrahedral amorphous carbon sensors and evaluation of their biofouling properties

Glutamate detection by amino functionalized tetrahedral amorphous carbon surfaces

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