Doped semiconductor nanocrystal based fluorescent cellular imaging probes

Maity, Amit Ranjan; Palmal, Sharbari; Basiruddin, SK; Karan, Niladri S.; Sarkar, Suresh; Pradhan, Narayan; Jana, Nikhil R.

doi:10.1039/c3nr00549f

Cited by 41 publications

(49 citation statements)

References 32 publications

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“…Q-dots may additionally be doped with other metal ions to give, for example, brightly fluorescent Mn-doped ZnS, Mn-doped ZnSe, or Cu-doped InZnS particles (10-80 nm in diameter) which represent a new class of fluorescent nanoparticles with low toxicity. 120 Aside from Q-dots composed of Zn(II), Cd(II), sulfide and selenide there are numerous other kinds of such particles. It is difficult to keep track with the variety of materials that have been presented in recent years.…”

Section: Metal Chalcogenide Quantum Dots (Classical Q-dots)mentioning

confidence: 99%

An overview of nanoparticles commonly used in fluorescent bioimaging

2015

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This article gives an overview of the various kinds of nanoparticles (NPs) that are widely used for purposes of fluorescent imaging, mainly of cells and tissues. Following an introduction and a discussion of merits of fluorescent NPs compared to molecular fluorophores, labels and probes, the article assesses the kinds and specific features of nanomaterials often used in bioimaging. These include fluorescently doped silicas and sol-gels, hydrophilic polymers (hydrogels), hydrophobic organic polymers, semiconducting polymer dots, quantum dots, carbon dots, other carbonaceous nanomaterials, upconversion NPs, noble metal NPs (mainly gold and silver), various other nanomaterials, and dendrimers. Another section covers coatings and methods for surface modification of NPs. Specific examples on the use of nanoparticles in (a) plain fluorescence imaging of cells, (b) targeted imaging, (c) imaging of chemical species, and (d) imaging of temperature are given next. A final section covers aspects of multimodal imaging (such as fluorescence/nmr), imaging combined with drug and gene delivery, or imaging combined with therapy or diagnosis. The electronic supplementary information (ESI) gives specific examples for materials and methods used in imaging, sensing, multimodal imaging and theranostics such as imaging combined with drug delivery or photodynamic therapy. The article contains 273 references in the main part, and 157 references in the ESI.

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Section: Metal Chalcogenide Quantum Dots (Classical Q-dots)mentioning

confidence: 99%

An overview of nanoparticles commonly used in fluorescent bioimaging

2015

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“…Mn-or Cu-doped Zn chalcogenide QDs, whichd on ot contain heavy metal ions, could potentially serve as fluorescent imaging probesw ith reduced toxicity. [116] Although silicon-based QDs are biocompatible and potentially biodegradable, their clinicala pplicationi s still questionable. Erogbogbo et al demonstrated that silicon QDs could be safely used at high doses (minimal cytotoxicity both in vitro and in vivo).…”

Section: In Vivo Toxicitymentioning

confidence: 99%

Quantum Dot‐Based Nanotools for Bioimaging, Diagnostics, and Drug Delivery

2016

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Quantum dots (QDs) are highly fluorescent nanocrystals with advanced photophysical and spectral properties: high brightness and stability against photobleaching accompanied by broad excitation and narrow emission spectra. Water-soluble QDs functionalized with biomolecules, such as proteins, peptides, antibodies, and drugs, are used for biomedical applications. The advantages of QD-based approaches to immuno-histochemical analysis, single-molecule tracking, and in vivo imaging (over traditional methods with organic dyes and fluorescent proteins) are explained. The unique spectral properties of QDs offer opportunities for designing systems for multiplexed analysis by multicolor imaging for the simultaneous detection of multiple targets. Conjugation of drug molecules with QDs or their incorporation into QD-based drug-delivery particles makes it possible to monitor real-time drug tracking and carry out image-guided therapy. Because of the tunability of their photophysical properties, QDs emitting in the near-infrared have become an attractive tool for deep-tissue mono- and multiphoton in vivo imaging. We review recent achievements in QD applications for bioimaging, targeting, and drug delivery, as well as challenges related to their toxicity and non-biodegradability. Key and perspectives for further development of advanced QD-based nanotools are addressed.

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“…However, presence of heavy metals in their composition limits their extensive and wider application in various in vivo therapeutic and clinical applications . Thus, alternative fluorescent nanoparticles similar to quantum dots are under intense investigation that includes carbon, silicon, doped semiconductor, and gold clusters …”

Section: Introductionmentioning

confidence: 99%

Gold nanoclusters with enhanced tunable fluorescence as bioimaging probes

Palmal

Jana

2013

WIREs Nanomed Nanobiotechnol

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Development of unique bioimaging probes offering essential information's about bio environments are an important step forward in biomedical science. Nanotechnology offers variety of novel imaging nanoprobes having high-photo stability as compared to conventional molecular probes which often experience rapid photo bleaching problem. Although great advances have been made on the development of semiconductor nanocrystals-based fluorescent imaging probes, potential toxicity issue by heavy metal component limits their in vivo therapeutic and clinical application. Recent works show that fluorescent gold clusters (FGCs) can be a promising nontoxic alternative of semiconductor nanocrystals. FGCs derived imaging nanoprobes offer stable and tunable visible emission, small hydrodynamic size, high biocompatibility and have been exploited in variety in vitro and in vivo imaging applications. In this review, we will focus on the synthetic advances and bioimaging application potentials of FGCs. In particular, we will emphasize on functional FGCs that are bright and stable enough to be useful as bioimaging probes.

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Doped semiconductor nanocrystal based fluorescent cellular imaging probes

Cited by 41 publications

References 32 publications

An overview of nanoparticles commonly used in fluorescent bioimaging

An overview of nanoparticles commonly used in fluorescent bioimaging

Quantum Dot‐Based Nanotools for Bioimaging, Diagnostics, and Drug Delivery

Gold nanoclusters with enhanced tunable fluorescence as bioimaging probes

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