We investigate the temperature dependence of the lower critical field Hc1(T ), the field at which vortices penetrate into the sample, of a high-quality fluorine-doped NdFeAsO single crystal under static magnetic fields H parallel to the c-axis. The temperature dependence of the first vortex penetration field has been experimentally obtained and pronounced changes of the Hc1(T) curvature are observed, which is attributed to the multiband superconductivity. Using a two-band model with s-wave-like gaps, the temperature-dependence of the lower critical field Hc1(T ) can be well described. These observations clearly show that the superconducting energy gap in fluorine-doped NdFeAsO is nodeless. The values of the penetration depth at T = 0 K have been determined and confirm that the pnictide superconductors obey an Uemura-style relationship between Tc and λ ab (0) −2 .PACS numbers: 74.20. Rp, 74.25Ha, 74.25.Dw, 74.25.Jb, 74.70.Dd Superconductivity in the iron-pnictide family has been studied intensively due to the comparably large transition temperatures T c of up to 55 K, the unconventional superconducting (SC) properties and interplay with various electronic ground states, such as nematic phase and magnetism [1][2][3][4][5][6]. One of the crucial issues in understanding the SC mechanism in pnictides is the pairing symmetry of the SC gap [7,8]. Although there is a general consensus that spin fluctuations play an important role in the formation of Cooper pairs in pnictides, many aspects such as the role of magnetism, the nature of chemical tuning, and the resultant pairing symmetry remain unsettled [9,10]. Recently, Chubukov and Hirschfeld have shown that there is no general consensus on the nature of pairing in iron-based superconductors leaving the perspectives ranging from s ++ wave, to s ± , and d-wave [11]. It is quite different from that of cuprates in which almost all have a nodal pairing state [12]. Different scenarios have been proposed to explain the mechanism of the superconductivity in pnictides which pointed to the existence of two-gap, isotropic and anisotropic s-wave, d-wave and even p-wave mechanism [13][14][15][16][17][18][19][20][21][22][23]. Such scattered pairing symmetries and various interpretations occur partly due to a sensitive dependence on measurement probes and material quality and stoichiometry.In view of the existing divergence of conclusions about the gap symmetry, there is a clear need to obtain a set of data by comprehensive study. Lower critical field (H c1 ), or equivalently, magnetic penetration depth (λ) is an excellent tool to address this question. The H c1 , i.e., the thermodynamic field at which the presence of vortices into the sample becomes energetically favorable, is a very useful parameter, providing key information regarding bulk thermodynamic properties and carrying information about the underlying pairing mechanism. Indeed, the gap properties of different families of pnictides have been investigated by tracking the H c1 and the magnetic penetration depth [24][25][26]...