Dormancy in grape buds: isolation and characterization of catalase cDNA and analysis of its expression following chemical induction of bud dormancy release

Or, Etti; Vilozny, Iris; Fennell, Anne; Eyal, Yoram; Ogrodovitch, Aliza

doi:10.1016/s0168-9452(01)00542-8

Cited by 67 publications

(52 citation statements)

References 24 publications

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“…In group RD, sequences related to metabolism and oxidation-reduction were the most abundant, which fits well with the idea that some basic metabolic activation is required to restart growth and cell division following dormancy and with S1 S2 S3 S4 S5 Z1 Z2 Z3 Z4 S1 S2 S3 S4 S5 Z1 Z2 Z3 S1 S2 S3 S4 S5 Z1 Z2 Z3 Z4 S1 S2 S3 S4 S5 Z1 Z2 Z3 those previous works linking oxidative stress and dormancy release (Nir et al, 1986;Scalabrelli et al, 1991;Or et al, 2002). On the other side, group DR was rich in stress and defence genes, as dormant tissues must be prepared against drought and cold stresses appearing during winter time.…”

Section: Discussionsupporting

confidence: 87%

Molecular aspects of dormancy in peach (Prunus persica [L.] Batsch.)

Leida¹

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En estos cuatros años en los que he estado trabajando, he conocido a mucha gente que me ha ayudado en distintas formas para la realización de esta tesis y por eso he pensado en agradecerlos.En primer lugar quería dar las gracias a mis directores por la dedicación que me han prestado. A Gerardo y Marisa por darme la oportunidad de trabajar en el IVIA y de desarrollar esta tesis. A Gabino por haber estado ahí en todo momento, para enseñar y contestar a mis preguntas, sin su apoyo no habría podido llegar hasta aquí. ABSTRACTDormancy is one of the most important adaptive mechanisms developed by perennial plants, in order to survive the low temperatures of autumn and winter in temperate climates. The study of the genes regulated during dormancy release is crucial to understand the process, with the final objective of the development of new varieties with a better adaptation to certain environments; and this is particularly important considering the increasing economical weight of fruit crops in low and medium chilling regions as the Mediterranean area. We focused on the molecular and physiological mechanisms underlying the maintenance and release of seasonal dormancy in peach. In order to achieve this we first used suppression subtractive hybridization (SSH) to identify genes expressed in dormant and dormancy-released buds in two cultivars with different chilling requirements, 'Zincal-5' and 'Springlady', and subsequently validated their differential expression utilizing a peach cDNA microarray platform containing transcripts enriched in flower buds. Additionally, we carried out a genome-wide search of peach genes related to dormancy release by hybridizing the previous cDNA microarray with mRNA samples from 10 cultivars showing different dormancy behaviour, followed by an expression correlation analysis.Among the most relevant genes identified in these two first works, we found the DORMANCY ASSOCIATED MADS-box genes DAM4, DAM5 and DAM6, described independently by other groups working in peach and other species. The central role of DAM genes in dormancy regulation has also been confirmed by additional functional approaches as the analysis of the non-dormant evg mutant, QTL analysis, and transgenic approaches.In our second work we focused on the molecular mechanisms of DAM6 down-regulation concomitant with dormancy release in flower buds. A ChIP analysis of DAM6 promoter and structural gene revealed chromatin modification events similar to those observed in vernalization of Arabidopsis and cereals. We showed that DAM6 is transcriptionally active in dormant buds collected in October, when a short chromatin region around its ATG was trimethylated in histone H3 at K4 (H3K4) and acetylated at the N-terminal tail of H3. Concomitantly with DAM6 repression, H3K4 became demethylated and H3 deacetylated. Later H3K27 was found trimethylated along a genomic region larger than 4kb, including promoter, coding sequence and intron. Due to their relevance in dormancy regulation, DAM genes could be used as expression markers to assess...

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Section: Discussionsupporting

confidence: 87%

Molecular aspects of dormancy in peach (Prunus persica [L.] Batsch.)

Leida¹

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“…Dicho producto se reporta que, en las primeras horas después de su aplicación en las plantas de vid, activa más de 800 genes dentro de la yema, pero se desconoce con certeza la secuencia de la activación de dichos genes Or et al (2002). El tener conocimiento del modo de activación de los genes en la yema, haría posible determinar el mecanismo de acción del producto en las yemas de las plantas de vid.…”

Section: Introductionunclassified

“…This product is reported to activate more than 800 genes within the yolk within the first few hours after its application to the grapevine plants, but the sequence of the activation of these genes is unknown. Or et al (2002). Knowing the mode of activation of the genes in the yolk would make it possible to determine the mechanism of action of the product in the buds of the vine plants.…”

Section: Introductionmentioning

confidence: 99%

“…Knowing the mode of activation of the genes in the yolk would make it possible to determine the mechanism of action of the product in the buds of the vine plants. At the biochemical level, it is now known that the product inhibits the enzyme catalase, thereby reducing the concentration of peroxide in the tissues, a reaction that triggers the processes that promote bud budding (Or et al, 2002;Osorio et al, 2004;Pérez and Lira, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, consecuencia la concentración de peróxido en los tejidos, reacción con lo cual se desencadenan los procesos que promueven la brotación de las yemas (Or et al, 2002;Osorio et al, 2004;Pérez y Lira, 2005).…”

Section: Introductionunclassified

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Efectos de dosis de cianamida de hidrógeno en brotación y producción de racimos en vid de mesa

Díaz

Blanco

2017

Remexca

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En regiones cálidas, como las áreas del Desierto de Sonora, el cultivo de la vid enfrenta el problema de insuficiencia en acumulación de unidades frio, condición que provoca que las variedades de vid broten tardíamente y en forma irregular. Para solventar este problema los productores hacen uso de promotores de brotación como la cianamida de hidrogeno, la cual es aplicada inmediatamente después de la poda, a finales de diciembre o principios de enero. Con el propósito de determinar el efecto de dosis crecientes de cianamida de hidrógeno en la brotación de las yemas y en la producción de racimos de la vid se realizaron varias pruebas experimentales durante durante los ciclos de producción del 2009, 2010, 2011 y 2013 en viñedos comerciales con el cultivar Perlette, irrigado con sistema presurizado tipo goteo.

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An FTIR study of the induction and release of kiwifruit buds from dormancy

et al. 2010

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BACKGROUND: Many deciduous, perennial fruit crops require winter chilling for adequate budbreak and flowering. Recent research has shown that changes in sugar and amino acid profiles are associated with the release of buds from dormancy. This paper uses FTIR spectrometry to provide an alternative mechanism for tracking metabolic changes in the meristems of kiwifruit buds during winter dormancy. The results suggest that the application of multivariate analysis to FTIR spectra has the potential to be a reliable and fast method for detecting structural and compositional changes in fruit crops.

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Dormancy in grape buds: isolation and characterization of catalase cDNA and analysis of its expression following chemical induction of bud dormancy release

Cited by 67 publications

References 24 publications

Molecular aspects of dormancy in peach (Prunus persica [L.] Batsch.)

Molecular aspects of dormancy in peach (Prunus persica [L.] Batsch.)

Efectos de dosis de cianamida de hidrógeno en brotación y producción de racimos en vid de mesa

An FTIR study of the induction and release of kiwifruit buds from dormancy

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