Dormant eggs of a calanoid copepod from tropical brackish aquaculture ponds

Beyrend-Dur, Delphine; Dur, Gaël; Souissi, Sami; Hwang, Jiang‐Shiou

doi:10.1163/15685403-00003284

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…Most studies on copepod resting eggs are conducted by extracting sediment (from cores) sampled in the field and subsequently incubating it at different environmental conditions that trigger hatching of the sedimented eggs (e.g., Marcus, 1984;Engel and Hirche, 2004;Masero andVillate, 2004, Dahms et al, 2006;Sichlau et al, 2011;Beyrend-Dur et al, 2014). In field studies, age determination of eggs is often done by isotopic analysis of 210 Pb and/or 137 Cs for estimating the age of the sediment in which the eggs reside (Dahms et al, 2006;Jiang et al, 2006;Katajisto, 2006;Sichlau et al, 2011).…”

Section: Common Approaches To Study Age and Viability Of Copepod Restmentioning

confidence: 99%

Copepod Embryonic Dormancy: “An Egg Is Not Just an Egg”

Hansen

2019

The Biological Bulletin

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Long-lasting embryonic dormancy in invertebrates defies our understanding of what constitutes life because, for example, eggs of some copepods can delay hatching for decades or even centuries. Copepods, often millimeter-sized crustaceans, are some of the most numerous multicellular organisms on earth and are key organisms in most aquatic food webs. Some important free-living marine and estuarine species overwinter or oversummer by arrested embryogenesis in dormancy. The present contribution discusses the complex mechanisms behind embryonic dormancy by compiling knowledge from the 42 calanoid copepods from the superfamily Centropagoidea with well-described embryonic dormancy, which has been of scientific interest for decades. However, the determination of categories of copepod resting eggs-that is, diapause and quiescence, transitions between categories, the mechanisms controlling arrested development by the embryos, and how they interact with their surroundings-is not fully understood. Moreover, a clear link between the presence of the free-swimming population and their resting eggs in sediments is still not convincingly demonstrated. Here I evaluate the relative significance of potential cues driving the production of and the phase shift between egg categories. Understanding the initiation and termination of embryonic dormancy is of great importance for fundamental science-that is, population and food web ecology as well as climate science, aquaculture live feed, and ballast water research. Molecular techniques are developing rapidly, especially within health sciences, thus providing relevant tools applicable for plankton research. Here I suggest that applying molecular methods in addition to traditional physiological approaches in future research will lead to greater understanding of copepod embryonic dormancy, one of nature's wonders.

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Section: Common Approaches To Study Age and Viability Of Copepod Restmentioning

confidence: 99%

Copepod Embryonic Dormancy: “An Egg Is Not Just an Egg”

Hansen

2019

The Biological Bulletin

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“…However, scarce contribution is paid on tropical copepod species. Beyrend‐Dur, Dur, Souissi and Hwang () were the first to report that the eggs (unidentified type) of the tropical calanoid copepod, Acartia bilobata , collected from sediments of a brackish aquaculture pond in southern Taiwan remain viable after cold storage. This species has great aquaculture potential because of their high productivity (Pan et al., ), and the feasibility of a quiescent cold‐induction protocol was preliminarily examined (Pan, Souissi, Hwang & Souissi, ).…”

Section: Introductionmentioning

confidence: 99%

Egg hatching rate and fatty acid composition of Acartia bilobata (Calanoida, Copepoda) across cold storage durations

et al. 2018

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To investigate egg storage capacity of the copepod Acartia bilobata for aquaculture interest, we tested hatching success rate (HSR) of inclusive eggs (mixture of all egg types) after 4°C storage. The HSR peaked after 14 days storage when incubating at 28°C for 48 hr (85.8 ± 1.6%) and 72 hr (87.6 ± 0.9%), then gradually declined until 1 year (48 hr: 7 ± 0.6%; 72 hr: 19.4 ± 3.9%). Reallocation of fatty acid profile suggests that docosahexaenoic acid (DHA) is correlated with the HSR of A. bilobata eggs. Additionally, we investigated the HSR of diapausing eggs (unhatched eggs after 72 hr incubation of the inclusive eggs) after 4°C storage. Their HSR peaked after 14 days storage (48 hr:75.3 ± 3.5%; 72 hr:78.2 ± 2.1%), then gradually declined until 60 days (48 hr HSR:42.1 ± 2.3%; 72 hr HSR:53.0 ± 3.2%). Overall, we illustrated the hatchability of diapausing and quiescent eggs of A. bilobata after 4°C storage. The cold storage capacities were low (<60% HSR after 60 days), and it could be limited by the egg DHA content. Our findings provide implications for future studies aiming to improve cold storage techniques of tropical copepod eggs for aquaculture applications. K E Y W O R D SAcartia bilobata, diapausing egg, egg cold storage, fatty acid, quiescent egg

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“…Hence, in a water column of a few meters depth, the tank sediments are likely to host vast amounts of copepod eggs of one or the other categories (Sørensen et al 2007). The accumulation of eggs in such a sediment egg bank has never been documented and quantified systematically before, but Beyrend-Dur et al (2014) and van der Meeren et al (2014) state that resting eggs can be found in aquaculture pond sediments at high concentrations and can hatch at refilling. If a substantial amount of eggs happens to be present in the tank sediment, these eggs could be stored and used as a source of newly hatched nauplii as live feed in the daily management of these facilities such as what is done with Artemia, and has been proposed earlier for copepods indoor productions: 'hatch and feed' .…”

Section: Introductionmentioning

confidence: 99%

Outdoor rearing facilities of free spawning calanoid copepods for turbot larva can host a bank of resting eggs in the sediment

Hansen

Blanda

Drillet³

et al. 2015

Aquacult Int

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It is well established in Denmark to rear calanoid copepods in outdoor tanks for use as live feed during turbot (Scophthalmus maximus) larval production. However, the copepod assemblages, composed of a mixture of all development stages and therefore body sizes, vary over time and do not always match the larval needs. When turbot larvae reach metamorphosis and are transferred indoor for weaning, the outdoor tank sediments may reveal vast amounts of copepod eggs undergoing dormancy. Here, we report a copepod species succession firstly among Centropages hamatus and then Acartia spp. both with resting eggs as part of their life cycles as a result of two different nutrients treatments and a control. We found a tendency to a higher egg production and indeed more eggs in the sediment of nutrient amended tanks. In fact close to 5 million eggs per square meter, making up to 400 million eggs per tank was found in the sediment after one production.Instead of discarding the sediment between production batches, we propose to collect it and generate an egg bank. These eggs can be stored for months to a year, however, according to the results, a large loss rate occurred, which could be potentially decreased by the optimization of storage conditions. Those procedures will enable hatchery managers to apply newly hatched copepod nauplii exactly when the turbot larvae start feeding which would potentially solve the, often occurring, mismatch between the time of start-feeding turbot larvae and actual available prey field.

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Dormant eggs of a calanoid copepod from tropical brackish aquaculture ponds

Cited by 7 publications

References 17 publications

Copepod Embryonic Dormancy: “An Egg Is Not Just an Egg”

Copepod Embryonic Dormancy: “An Egg Is Not Just an Egg”

Egg hatching rate and fatty acid composition of Acartia bilobata (Calanoida, Copepoda) across cold storage durations

Outdoor rearing facilities of free spawning calanoid copepods for turbot larva can host a bank of resting eggs in the sediment

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