Dormant forms of Micrococcus luteus and Arthrobacter globiformis not platable on standard media

Mulyukin, A. L.; Demkina, E. V.; Kryazhevskikh, N. A.; Suzina, N. E.; Vorob'eva, L. I.; Дуда, В. И.; Гальченко, В. Ф.; Эль-Регистан, Г. И.

doi:10.1134/s0026261709040031

Cited by 26 publications

(7 citation statements)

References 20 publications

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“…The extreme manifestation of this effect is a loss of bacterial cells' capacity to form colonies on solid media, i.e. the development of the nonculturable state [25]. However, this is accompanied by the pro cesses resulting in AHB dependent stabilization of cell biopolymers including the DNA, which become highly resistant to a wide variety of stress factors, including UV irradiation.…”

Section: Discussionmentioning

confidence: 99%

“…This could be due to the fact established earlier that long chain AHBs operate as anabiosis autoinducers. Increasing their concentrations results in the develop ment of the hypometabolic (10 -4 M) and then ameta bolic (10 -3 M) cell state [2,18,25]. This accounts for a decrease in the SOS system activity that was caused by a reduction in the general metabolic activity deter mined from the decrease in F i value at AHB concen trations over 10 -5 M. Interestingly, the short chain C7 AHB that does not affect the development of the anabiotic state failed to induce the SOS response of the test strain, regardless of the concentration applied.…”

Section: Discussionmentioning

confidence: 99%

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Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

et al. 2012

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This work is concerned with the role of alkylhydroxybenzenes (AHBs), chemical analogs of the autoregulatory microbial d 1 factors, on the development of the stress response of bacterial cells to UV irradi ation, including SOS system induction, preservation of cell viability, and S R phase transitions of the Escherichia coli test strain with the bioluminescence genes cloned under the control of the recA gene pro moter. UV irradiation, a natural stress factor, and an increase in AHB concentrations were found to elicit uni form responses in bacteria, indicating that AHBs function as alarmones, i.e., alarm signals. It was revealed that preincubating bacteria with alkylhydroxybenzenes considerably enhanced their viability upon irradiation with lethal UV doses; this was accompanied by a relative decrease in the SOS response activity and a concom itant increase in the frequency of phase transitions. The efficiency of the protective action of AHBs increased with an increase in their hydrophobicity degree. The probable mechanism of the protective effect of AHBs is discussed, based on their capacity for the interaction with biopolymers, which results in changing their struc tural organization and conferring resistance to a broad spectrum of stress factors. Such a "passive" protective mechanism reduces the susceptibility of DNA to UV irradiation, causing a decrease in the parameters related to the SOS system induction that is responsible for the "active" protective mechanism in bacterial cells. As a result, viability retention under the lethal influence of UV irradiation is possible at minimal values of repair activity and is accompanied by an increase in the phenotypic variability of the surviving part of a bacterial population.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

et al. 2012

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“…Under stress and starvation conditions like those in dry bentonites, microorganisms exhibit a number of physiological responses, including cell dewatering, mineralization, rebuilding of specific cell ultrastructures, down-sizing, accumulation of protective intracellular metabolites, reduction or alteration in physiological activity and entry into long-term dormancy ( Mulyukin et al., 2001 ; Suzina et al., 2006 ). Formation of specialized endospores by bacilli and clostridia ( Paredes-Sabja et al., 2011 ), cyanobacterial akinetes ( Billi, 2012 ), and other dormant forms of Gram-positive and Gram-negative bacteria also enhances microbial stress tolerance ( Soina et al., 2004 ; Mulyukin et al., 2008 , 2009 ). Reliable reports exist of the resurrection of dormant cells, for instance clostridial and bacilli endospores, from various desiccated and heat-treated environmental samples ( Kennedy and Swierczynski, 1994 ; O'Sullivan et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Bacterial diversity and production of sulfide in microcosms containing uncompacted bentonites

Grigoryan

Jalique

Medihala

et al. 2018

Heliyon

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AimsThis study examined the diversity and sulfide-producing activity of microorganisms in microcosms containing commercial clay products (e.g., MX-80, Canaprill and National Standard) similar to materials which are currently considered for use in the design specifications for deep geologic repositories (DGR) for spent nuclear fuel.Methods and resultsIn anoxic microcosms incubated for minimum of 60 days with 10 g l-1 NaCl, sulfide production varied with temperature, electron donor and bentonite type. Maximum specific sulfide production rates of 0.189 d−1, 0.549 d−1 and 0.157 d−1 occurred in lactate-fed MX-80, Canaprill and National Standard microcosms, respectively. In microcosms with 50 g l-1 NaCl, sulfide production was inhibited. Denaturing gradient gel electrophoresis (DGGE) profiling of microcosms revealed the presence of bacterial classes Clostridia, Bacilli, Gammaproteobacteria, Deltaproteobacteria, Actinobacteria, Sphingobacteriia and Erysipelotrichia. Spore-forming and non-spore-forming bacteria were confirmed in microcosms using high-throughput 16S rRNA gene sequencing. Sulfate-reducing bacteria of the genus Desulfosporosinus predominated in MX-80 microcosms; whereas, Desulfotomaculum and Desulfovibrio genera contributed to sulfate-reduction in National Standard and Canaprill microcosms.ConclusionsCommercial clays microcosms harbour a sparse bacterial population dominated by spore-forming microorganisms. Detected sulfate- and sulfur-reducing bacteria presumably contributed to sulfide accumulation in the different microcosm systems.Significance and impact of studyThe use of carbon-supplemented, clay-in-water microcosms offered insights into the bacterial diversity present in as-received clays, along with the types of metabolic and sulfidogenic reactions that might occur in regions of a DGR (e.g., interfaces between the bulk clay and host rock, cracks, fissures, etc.) that fail to attain target parameters necessary to inhibit microbial growth and activity.

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“…Dormant forms of bacteria were obtained using post stationary cultures (with an age up to 4 months) that developed under quasi natural conditions as described in [17,18]. The dormant forms of Arthro bacter strains were obtained by growing them (i) in standard LB medium, with subsequent storage of the developed culture at 28°C for 2 months; (ii) in a nitro gen limited medium, with subsequent storage at 4°C for 2 months; and (iii) in a medium with a phosphate imbalance, with subsequent storage at 28°C for 2 months [19].…”

Section: Methodsmentioning

confidence: 99%

Applicability of MALDI mass spectrometry for diagnostics of phase variants in bacterial populations

et al. 2015

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Efficiency of MALDI mass spectrometry for differentiation between phenotypic phase variants (in colony morphology and virulence/avirulence) was investigated for saprotrophic and opportunistically pathogenic bacteria of five genera (Acinetobacter, Arthrobacter, Rhodococcus, Corynebacterium, and Escheri chia). Analysis of MALDI spectra (on the SA and HCCA matrices) included (1) determination of similarity of the protein spectra as a percentage of the common protein peaks to the total amount of proteins, which reflects the phylogenetic relationships of the objects and has been recommended for identification of closely related species; (2) comparison of intensities of the common peaks; and (3) the presence of specific peaks as determinative characteristics of the variants. Under the standard analytical conditions, the similarity between the MALDI profiles was shown to increase in the row: genus-species-strain-variant. Assessment of inten sities of the common peaks was most applicable for differentiation between phase variants, especially in the case of high similarity of their profiles. Phase variants (A. oxydans strain K14) with similar colony morpho types (S, R, M, and S m ) grown on different media (LB agar, TSA, and TGYg) exhibited differences in their protein profiles reflecting the differences in their physiological characteristics. This finding is in agreement with our previous results on screening of the R. opacus with similar colony morphology and different substrate specificity in decomposition of chlorinated phenols. Analysis of MALDI spectra is probably the only efficient method for detection of such variants.

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Dormant forms of Micrococcus luteus and Arthrobacter globiformis not platable on standard media

Cited by 26 publications

References 20 publications

Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

Involvement of alkylhydroxybenzenes in the Escherichia coli response to the lethal effect of UV irradiation

Bacterial diversity and production of sulfide in microcosms containing uncompacted bentonites

Applicability of MALDI mass spectrometry for diagnostics of phase variants in bacterial populations

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