Dorsal raphe organization

Potassium Channel Conductance Is Involved in Phenylephrine-Induced Spontaneous Firing of Serotonergic Neurons in the Dorsal Raphe Nucleus

“…However, these neurons with spontaneous firing were found mostly to be either dopaminergic neurons or glutamatergic neurons as verified by the single-cell PCR post the electrophysiological recordings ( n = 8, 80% dopaminergic neurons; n = 1, 10% glutamatergic neurons). The dopamine and glutamatergic neurons in DRN were also reported in the literature ( Soiza-Reilly and Commons, 2011 ; Matthews et al, 2016 ; Commons, 2020 ). Therefore, neurons exhibiting spontaneous activity were not included for data analysis.…”

Sex-specific regulation of binge drinking, social, and arousal behaviors by subcortical serotonin 5HT2creceptor-containing neurons

“…Previous anatomical and functional evidence suggests that the DRN is comprised of parallel sub-systems that differ in their input-output connectivity, physical localization within the DRN, neurotransmitter co-release properties, and roles in behavior (Commons, 2020; Gagnon and Parent, 2014; Huang et al, 2019; Muzerelle et al, 2016; Okaty et al, 2020; Ren et al, 2018; Sengupta and Holmes, 2019). Here, we show that two distinct output nodes of a DRN sub-system, the BNST and the LHb, function in concert to modulate anxiety-like behavior and negative emotional states.…”

“…Retrograde and anterograde tracing studies suggest that the DRN is anatomically organized along both dorsal/ventral and rostral/caudal axes (Commons, 2020; Muzerelle et al, 2016), and that single DRN neurons collateralize to send inputs to multiple different downstream target regions (Gagnon and Parent, 2014). In agreement with these previous studies, our retrograde tracing experiments revealed that the LHb and the BNST receive DRN inputs from many of the same individual 5-HT neurons, which were primarily located in the caudal and dorsal aspect of the DRN.…”

“…5-HT neurons are located primarily in hindbrain raphe nuclei, specifically the dorsal (DRN) and median (MRN) raphe nuclei. These neurons project widely throughout the brain, including to cortical, amygdalar, midbrain, and other hindbrain regions (for review, see (Commons, 2020)). A recent anatomical and functional mapping study of the DRN revealed that there are distinct functional populations of 5-HT neurons localized to discrete sub-regions of the DRN (Ren et al, 2018).…”

Electrophysiological Characteristics of Dorsal Raphe Nucleus in Tail Suspension Test

“…The relationship between depression-like behavior and electrophysiology heterogeneity of 5-HT neurons in DRN remains unknown. Although 5-HT neurons are traditionally considered to be homogeneous, they are different both in molecular identity and in electrophysiological property (Cohen et al, 2015 ; Okaty et al, 2015 , 2020 ; Fernandez et al, 2016 ; Commons, 2020 ), which suggests that there exist distinct functional subtypes in behavioral coding. Moreover, electrophysiology signal recording at first hand has higher resolution than calcium imaging recording that converted to fluorescence signal.…”