Dosage Suppressor of NNF1(DSN1)Promotes Tumour Progression of Oral Squamous Cell Carcinoma(OSCC)Through Remaining High Level of Chromosome Instability

Abstract

Introduction: Dosage suppressor of NNF1 (DSN1) is a component of the kinetochore protein complex which is critical for normal chromosome segregation. Researches showed that DSN1 was upregulated in colorectal carcinoma and hepatocellular carcinoma. However, the biofunction and potential mechanism was still unknown. Materials and methods: We searched The Cancer Genome Atlas (TCGA) database and analyse DSN1 expression pattern in head and neck carcinoma. qRT-PCR and western blot were applied to measure the expression level of DSN1 in OSCC patients and OSCC cell lines. The biological influence of DSN1 was studied using CCK-8, colony formation assay, Transwell migration and invasion assays, the chromosome instability and DNA repair ability were also detected. Results: DSN1 was upregulated in OSCC tissues in both TCGA database and our in-house database. The proliferation, migration and invasion ability decreased in Cal-27 cell line after knocking down of DSN1. The epithelial-mesenchymal transition was activated in DSN1 overexpressed cells. DSN1 confers radio-resistance to OSCC and DSN1 remains high level of chromosome instability and low level of DNA repair. Conclusion: Taken above, our results indicate that the upregulated DSN1 is positively associated with prognosis in OSCC. Our findings indicate that DSN1 promotes OSCC cell proliferation, migration and invasion and radio resistance and remains OSCC with high level of chromosome instability.