Dose-Dependent Antimicrobial Activity of Silver Nanoparticles on Polycaprolactone Fibers against Gram-Positive and Gram-Negative Bacteria

Abstract

The adhesion ability and adaptability of bacteria, coupled with constant use of the same bactericides, have made the increase in the diversity of treatments against infections necessary. Nanotechnology has played an important role in the search for new ways to prevent and treat infections, including the use of metallic nanoparticles with antibacterial properties. In this study, we worked on the design of a composite of silver nanoparticles (AgNPS) embedded in poly-epsilon-caprolactone nanofibers and evaluated its antimicrobial properties against various Gram-positive and Gram-negative microorganisms associated with drug-resistant infections. Polycaprolactone-silver composites (PCL-AgNPs) were prepared in two steps. The first step consisted in the reduction in situ of Ag+ions using N,N-dimethylformamide (DMF) in tetrahydrofuran (THF) solution, and the second step involved the simple addition of polycaprolactone before electrospinning process. Antibacterial activity of PCL-AgNPs nanofibers againstE. coli,S. mutans,K. pneumoniae,S. aureus,P. aeruginosa, andB. subtiliswas evaluated. Results showed sensibility ofE. coli,K. pneumoniae,S. aureus, andP. aeruginosa, but not forB. subtilisandS. mutans. This antimicrobial activity of PCL-AgNPs showed significant positive correlations associated with the dose-dependent effect. The antibacterial property of the PCL/Ag nanofibers might have high potential medical applications in drug-resistant infections.

Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity

“…Pazos-Ortiz et al reported the dose-dependent antimicrobial activity of a composite of AgNPs embedded in poly-epsilon-caprolactone nanobers and tested against both Gram-positive and Gramnegative bacteria. 149 It was found that beginning from the 12.5 mM nanoparticle concentration, AgNPs gradually displayed a signicant antibacterial effect on the different bacterial strains. Similarly, Gurunathan et al demonstrated the dosedependent bactericidal activity of AgNPs at concentrations ranging from 0.1 to 1.0 mg ml À1 against two Gram-negative and two Gram-positive bacterial strains.…”

Antifungal susceptibility of Candida species to copper oxide nanoparticles on polycaprolactone fibers (PCL-CuONPs)

“…The administration of CuONPs as an antimicrobial treatment needs a vehicle, being the polymer films the most widely used materials in this area. The integration of metal oxide nanoparticles improves its antimicrobial as well as its physical, chemical and mechanical properties [10].…”

Development of Bionanocomposites Based on PLA, Collagen and AgNPs and Characterization of Their Stability and In Vitro Biocompatibility

“…Silver nanoparticles were reported to be toxic to human cells at concentrations above 1% [53] and induce the reactive oxygen species generation [54]. It has been reported that the cytotoxicity of antimicrobial medical devices containing silver nanoparticles is dependent on the size and surface area of inorganic ions released from the devices [55].…”