Dose-dependent genotoxic effect of pan masala and areca nut in the Salmonella typhimurium assay

Polasa, Kalpagam; Babu, S.; Shenolikar, I.S.

doi:10.1016/0278-6915(93)90160-z

Cited by 18 publications

(8 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mutagenic potential of ethanolic (Bagwe et al, 1990) and aqueous extracts (Polasa et al, 1993) of different brands of pan masala has also been reported. However, no information exists regarding the neoplastic potential of pan masala.…”

mentioning

confidence: 99%

Evaluation of carcinogenic/co-carcinogenic activity of a common chewing product, pan masala, in mouse skin, stomach and esophagus

et al. 1998

View full text Add to dashboard Cite

mentioning

confidence: 99%

Evaluation of carcinogenic/co-carcinogenic activity of a common chewing product, pan masala, in mouse skin, stomach and esophagus

et al. 1998

View full text Add to dashboard Cite

“…Katha also induced dominant lethal mutations leading to an increased number of post implantation loss in mice (Giri et al, 1988). Polasa et al (1993) have also reported weak mutagenecity response in Salmonella typhimurium by pan masala. Chromosome-damaging effects of pan masala were also assessed on cultured Chinese hamster ovary cells (Jaju et al, 1992;Patel et al, 1994).…”

Section: Discussionmentioning

confidence: 96%

Evaluation of genotoxicity of pan masala employing chromosomal aberration and micronucleus assay in bone marrow cells of the mice

et al. 2009

View full text Add to dashboard Cite

Pan masala is commonly consumed in south-east Asian and other oriental countries as an alternate of tobacco chewing and smoking. Genotoxic potential of pan masala (pan masala plain and pan masala with tobacco known as gutkha) was evaluated employing chromosome aberration (CA) and micronucleus (MN) assay in vivo. Animals were exposed to three different doses (0.5%, 1.5% and 3%) of pan masala plain (PMP) and gutkha (PMT) through feed for a period of 6 months and micronucleus and chromosomal aberrations were studied in the bone marrow cells. Induction of mean micronuclei in polychromatic erythrocytes (MNPCE) and normochromatic erythrocyte (MNNCE) was higher in both types of pan masala treated groups with respect to control group. Both pan masala plain and gutkha treatment significantly induced the frequency of MNPCE and MNNCE in the bone marrow cells, indicating the genotoxic potential. Furthermore, slight decline in the ratio of polychromatic erythrocytes to normochromatic erythrocytes was also noticed, suggesting the cytotoxic potential even though the ratio was statistically non significant. A dose-dependent, significant increase in chromosome aberration was observed in both types of pan masala treated mice with respect to control. However, no significant difference in micronucleus and chromosomal aberration induction was noticed between two types of pan masala exposed (PMP and PMT) groups. Results suggest that both types of pan masala, i.e. plain and gutkha, have genotoxic potential.

show abstract

“…Exposure to BN extracts was found to induce mutations at the hypoxanthine phosphoribosyltransferase (HPRT) locus in human keratinocytes (HaCaT cells), which also increased frequency of appearance of micronuclei, intracellular levels of reactive oxygen species and 8-hydroxyguanosine in the cells suggesting that stress caused by long-term BN extract exposure enhanced oxidative stress and genetic damage in HaKaT cells (Lai and Lee, 2006). When aqueous extracts of different brands of pan masala and scented BN or supari were tested for mutagenicity by the Salmonella typhimurium assay using tester strains TA98 and TA100 their mutagenic effects were found to be similar to that produced by BN extracts (Polasa et al, 1993). A study involving patients of head and neck cancer suggested that BQ chewing may increase mitochondrial DNA (mtDNA) mutation in human oral tissues and that accumulation of mtDNA deletions and subsequent cytoplasmic segregation of these mutations during cell division could be important contributors to the early phase of oral carcinogenesis (Lee et al, 2001).…”

Section: Betel Nut and Betel Nut Extracts In Carcinogenesismentioning

confidence: 88%

Betel Nut and Susceptibility to Cancer

Sharan

Choudhury

2010

Environmental Factors, Genes, and the Development of Human Cancers

View full text Add to dashboard Cite

Betel nut is a widely masticated natural product, which is consumed by over 600 million people across the globe. The ancient habit of betel nut chewing, either as dry or raw/wet nut, in association with betel leaf and a host of region specific additives, including chewing tobacco, is believed to be an important etiological factor for human cancer. Alkaloids and their betel nut specific nitrosamine derivatives produced upon metabolic activation interact with DNA and other cellular targets to produce highly variable mutagenic, genotoxic, cytostatic, immunostatic and teratogenic effects. At molecular level the betel nut or its constituents strongly influence gene expression patterns, especially that of tumor suppressor genes. Structural damage to nucleus and mitochondria, etc. are also induced. The review dwells upon these aspects of betel nut induced carcinogenesis to show that genetic susceptibility to cancer through generations progressively increased due to exposure to betel nut.

show abstract

Dose-dependent genotoxic effect of pan masala and areca nut in the Salmonella typhimurium assay

Cited by 18 publications

References 13 publications

Evaluation of carcinogenic/co-carcinogenic activity of a common chewing product, pan masala, in mouse skin, stomach and esophagus

Evaluation of carcinogenic/co-carcinogenic activity of a common chewing product, pan masala, in mouse skin, stomach and esophagus

Evaluation of genotoxicity of pan masala employing chromosomal aberration and micronucleus assay in bone marrow cells of the mice

Betel Nut and Susceptibility to Cancer

Contact Info

Product

Resources

About