Dose‐dependent inhibition of cell proliferation induced by lipid peroxidation products in rat hepatoma cells after enrichment with arachidonic acid

Muzio, Giuliana; Salvo, Raffaella A.; Trombetta, Antonella; Autelli, Riccardo; Maggiora, Marina; Terreno, Mario; Dianzani, Mario U.; Canuto, Rosa Angela

doi:10.1007/s11745-999-0416-2

Cited by 30 publications

(12 citation statements)

References 36 publications

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“…Our finding of noticeable anti-proliferative effect exhibited by ALA and LA treatment, concurs with literature [Lima et al, 2002], suggesting that ALAand LA-induced toxicity is mainly responsible for butterfat-induced antiproliferative effects. Moreover, we found that ALA and LA are mostly responsible for prooxidant-induced antiproliferative effect, which is further supported by data indicating the close relationship between oxidative stress with growth inhibitory effect [Hammer et al, 1997] and 3 H-thymidine incorporation [Muzio et al, 1999]. However, in our study OBF-mediated growth inhibition is not merely the consequence of induced cytotoxicity.…”

Section: Discussionsupporting

confidence: 90%

Butterfat fatty acids differentially regulate growth and differentiation in Jurkat T‐cells

Bergamo

Luongo

Maurano

et al. 2005

J of Cellular Biochemistry

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Synthetic Conjugated Linoleic Acid mixture (CLA; c9,t11; t10,c12-18:2) has been previously shown to inhibit growth, and enhance apoptosis and IL-2 mRNA synthesis in human lymphoblastic Jurkat T-cells. In this study, two different butterfat types were evaluated and compared for their effects on Jurkat cell viability, oxidative stress, pro-apoptotic activity, and cytokine synthesis: the conventionally produced butterfat (CBF), and organic butterfat (OBF) containing significantly higher amounts of c9,t11 (Rumenic Acid, RA), trans-vaccenic acid (VA; t11-18:1), alpha-linolenic acid (ALA), and lower levels of linoleic acid (LA). Results from cell treatment with both butterfat mixtures showed comparable oxidative stress (superoxide production, intracellular GSH depletion,and lipid peroxides yield), NADPH oxidase activation, cytotoxicity (LDH release), and IL-2 transcript level, whereas the effects of enhanced growth-inhibitory and pro-apoptotic activities were associated with OBF treatment. To then investigate each butterfat-induced effect caused by RA, VA, LA, and ALA, cells were exposed to synthetic FA concentrations similar to those from the different butterfats. Higher oxidative stress (superoxide production, intracellular GSH depletion) was induced by alpha-linolenic (ALA) and linoleic (LA) incubation (P<0.01) and superoxide production was suppressed by specific PKCalpha inhibitor (Gö 6976) and linked to increased toxicity and IL-2 synthesis inhibition. By contrast, cell treatment with RA increased apoptosis and IL-2 synthesis. These results suggest that a supply of ALA and LA is responsible for BF-induced oxidative stress via PKCalpha-NADPH oxidase pathway, and that enhanced antiproliferative effects in OBF treated cells is essentially determined by RA-induced pro-apoptotic activity.

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Section: Discussionsupporting

confidence: 90%

Butterfat fatty acids differentially regulate growth and differentiation in Jurkat T‐cells

Bergamo

Luongo

Maurano

et al. 2005

J of Cellular Biochemistry

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“…Lipid peroxidation was estimated by determination of the MDA concentration by the thiobarbiturate assay, as described by others (8,16). Briefly, a 0.1-ml aliquot of each sample was mixed with 0.9 ml of trichloroacetic acid (20%; Merck), and the mixture was centrifuged at 12,000 ϫ g and 4°C for 10 min.…”

Section: Animalsmentioning

confidence: 99%

Immunomodulatory Clarithromycin Treatment of Experimental Sepsis and Acute Pyelonephritis Caused by Multidrug-Resistant Pseudomonas aeruginosa

Giamarellos‐Bourboulis¹,

Adamis

Laoutaris³

et al. 2004

Antimicrob Agents Chemother

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Clarithromycin was administered intravenously to 55 rabbits to evaluate its effect on experimental sepsis caused by multidrug-resistant Pseudomonas aeruginosa. Acute pyelonephritis was induced after ligation of the right ureter and injection of 10 8 CFU of the test isolate per kg of body weight into the renal pelvis. The animals were divided into six groups: group A, controls; group B, rabbits that received one intravenous dose of 80 mg of clarithromycin per kg concomitantly with bacterial challenge; group C, rabbits that received two doses of clarithromycin, the second one of which was given 2 h after the first one; group D, rabbits that received 15 mg of amikacin per kg; group E, rabbits that received one dose of clarithromycin and amikacin; and group F, rabbits that received two doses of clarithromycin and amikacin. Serum endotoxin levels were estimated by the QCL-1000 Limulus amoebocyte lysate assay, tumor necrosis factor alpha (TNF-␣) levels were measured by a bioassay, and malondialdehyde (MDA) levels were measured by the thiobarbiturate assay. Viable bacterial counts in various tissue samples were also assessed. The mean survival times of the animals in groups A, B, C, D, E, and F were 4.50, 7.69, 4.07, 4.55, 11.55, and 11.60 days, respectively (P ‫؍‬ 0.033 for group D versus group F, P ‫؍‬ 0.006 for group D versus group E, P ‫؍‬ not significant for group B versus group E, P ‫؍‬ 0.042 for group C versus group F). Serum endotoxin levels were similar between groups at all sampling times; TNF-␣ and MDA levels in groups B, C, E, and F decreased significantly over follow-up. The numbers of viable bacterial cells in the infected kidney were similar among the groups; those in the liver, spleen, lungs, and mesenteral lymph nodes were significantly decreased in groups B, E, and F compared to those in groups A and D. It is concluded that a prolongation of survival in animals with experimental sepsis caused by multidrug-resistant P. aeruginosa was achieved after coadministration of clarithromycin and amikacin and that the increased survival was probably attributable to the immunomodulatory properties of clarithromycin.Clarithromycin is a macrolide classically known to possess an antimicrobial spectrum that includes gram-positive cocci and atypical pathogens (23). However, an increasing body of evidence suggests that clarithromycin possesses considerable antiinflammatory and immunomodulatory properties, recommending its administration for the treatment of chronic inflammatory conditions like diffuse panbronchiolitis and cystic fibrosis (11,21,26). Its immunomodulatory properties are observed in vitro at concentrations close to 10 g/ml (14). On the basis of that finding, intravenous administration of clarithromycin leading to the same levels in blood might be beneficial for the treatment of an acute inflammatory state like sepsis.Pseudomonas aeruginosa is a common pathogen that is involved in nosocomial sepsis and that is often characterized nowadays by multidrug resistance (18). In the present study, intravenous clar...

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“…Lipid peroxidation in serum was assessed by estimation of the concentration of malondialdehyde according to the thiobarbiturate assay, as described by others [4,5]. Briefly, a 0.1 ml aliquot of each sample was mixed with 0.9 ml of trichloroacetic acid 20% (Merck, Darmstadt, Germany) and centrifuged at 12,000 × g for 10 min at 4 • C. The supernatant was incubated with 1 ml of PBS (Merck) and 1 ml of thiobarbituric acid 0.6% (Merck) for 20 min at 90 • C. Optical density was then read at 535 nm (Hitachi Spectophotometer).…”

Section: Assay For Mda and Tnfαmentioning

confidence: 99%

Experimental sepsis using Pseudomonas aeruginosa: the significance of multi-drug resistance

Giamarellos‐Bourboulis¹

2004

International Journal of Antimicrobial Agents

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Dose‐dependent inhibition of cell proliferation induced by lipid peroxidation products in rat hepatoma cells after enrichment with arachidonic acid

Cited by 30 publications

References 36 publications

Butterfat fatty acids differentially regulate growth and differentiation in Jurkat T‐cells

Butterfat fatty acids differentially regulate growth and differentiation in Jurkat T‐cells

Immunomodulatory Clarithromycin Treatment of Experimental Sepsis and Acute Pyelonephritis Caused by Multidrug-Resistant Pseudomonas aeruginosa

Experimental sepsis using Pseudomonas aeruginosa: the significance of multi-drug resistance

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