Dose‐dependent initiation of myogenesis by neurotrophic factor

Popiela, Heinz; Ellis, Stanley; Festoff, Barry W.

doi:10.1002/jnr.490080240

Cited by 13 publications

(5 citation statements)

References 63 publications

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“…Cell culture Primary chick muscle cells were cultured in the presence of NTF as previously described (Popiela and Ellis, 1981;Popiela et al, 1982). Muscle cells were dissociated from 11-day-old chick embryo leg muscle tissue in 0.05% collagenase (Millipore, San Francisco, CA).…”

Section: Methodsmentioning

confidence: 99%

“…Two types of tissue extracts are generally added to the culture medium for growth of primary chick muscle cells. Traditionally, chick embryo extract is chosen (Konigsberg, 1979), or more recently a n aqueous extract of adult chicken ischiatic-peroneal nerves (NE) (Popiela, 1978), or its purified derivative (NTF) (Popiela and Ellis, 1981;Popiela et al, 1982), is substituted instead. The requirement for chick embryo extract was identified by its stimulatory effects on muscle cell proliferation (Slater, 1976), a requirement that is similarly fulfilled by NE or NTF.…”

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confidence: 99%

“…NTF is structurally as well as biologically similar to a variety of chicken transferrins (Beach et al, 1983). In the absence of NTF, muscle cell proliferation does not occur when cells are plated at low densities, although the medium contains 10% horse serum (horse transferrin), human transferrin, and insulin (Popiela et al, 1982).…”

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confidence: 99%

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Regulation of mitotic activity and the cell cycle in primary chick muscle cells by neurotransferrin

Popiela

Taylor

Ellis

et al. 1984

Journal Cellular Physiology

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We previously demonstrated that neurotransferrin (NTF), a transferrin extracted from adult chicken peripheral nerves, promotes growth of primary chick muscle cells in the absence of embryo extract. NTF was shown to stimulate DNA synthesis and cell proliferation. In the present study, we demonstrate that NTF is a mitogen using two independent methods; counts of orcein-stained mitotic figures and analysis of cell cycle kinetics with a fluorescence-activated cell sorter. In low-density cultures mitotic activity increases with increasing doses of NTF followed by a plateau at concentrations greater than 6 micrograms/ml. Residual, embryonic mitotic activity progressively declines with time after plating muscle cells in the absence of NTF. Absence of NTF for 2 days causes cells to lose irreversibly their myogenic potential. In the presence of NTF, mitotic activity increases for 2 days followed by a decline concurrent with myoblast fusion and formation of myotubes. Cell cycle analysis showed that NTF addition causes cell populations to shift from G1 to S and G2 + M within 18.5 hr. Muscle cells, plated at high densities in the absence of NTF, show mitotic activities similar to those plated at low densities in the presence of NTF. Addition of NTF to high-density cultures is ineffective in stimulating mitosis. These studies show that at typical cell plating densities, NTF is a required mitogen for primary chick muscle cell cultures.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Regulation of mitotic activity and the cell cycle in primary chick muscle cells by neurotransferrin

Popiela

Taylor

Ellis

et al. 1984

Journal Cellular Physiology

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“…The active substance in these various preparations has now been shown to be identical, or nearly so, to the circulating iron transport protein, transferrin (Tf) (Ii et al, 1982;Ozawa et al, 1981;Popiela et al, 1982;Beach et al, 1983Beach et al, , 1985. In addition to iron transport, Tf is required for the growth and development of a number of mammalian clonal cell lines and several primary cell culture systems also appear to require Tf Sato, 1979, 1985).…”

Section: Introductionmentioning

confidence: 99%

Monoclonal antibody detects embryonic epitope specific for nerve‐derived transferrin

Festoff

Muñoz

Patel

et al. 1989

J of Neuroscience Research

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Monoclonal antibodies were generated against transferrin purified from chick embryo extract by fusing spleen cells from BALB/c mice immunized against embryonic transferrin, with myeloma cells. Antibodies produced by the selected hybridoma clones were all type IgG. Twelve clones were selected for secretion of antibodies to the embryo extract-derived transferrin, and three clones were studied extensively. Immunoblotting was used to demonstrate antibody binding to several avian transferrin proteins derived from adult chicken serum, adult chicken peripheral nerves, and ovotransferrin. Screening and detailed epitope analysis were accomplished by solid-phase immunoassay. The results indicated that two clones, 2G9.1 and 2B11.1, recognized the embryonic and egg antigens in preference to the adult proteins. However, a third clone, 6H2.1, recognized the nerve-derived transferrin preferentially to both the embryonic and adult serum antigens. None of the clones recognized the serum-derived transferrin in preference to the other antigens. These results indicate that embryonic epitope(s) are conserved in the nerve- but not the serum-derived transferrin. They also show that the neural antigen has site(s) distinct from the embryonic proteins. No changes in displacement curves were observed after these proteins were digested with neuraminidase, indicating that the epitope differences discovered are not intimately related to sialic acid residues on the various transferrins.

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“…The importance of the extracellular matrix in skeletal-muscle development has been well established (Hauschka & Konigsberg, 1966;Konigsberg, 1970; Hauschka, 1972;Hauschka & White, 1972;De La Haba et al, 1975;Lipton, 1977;Puri et al, 1980;Chiquet et al, 1981;Ehrisman et al, 1981;Popiela et al, 1982;Beach et al, 1983).…”

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Extracellular-matrix synthesis by skeletal muscle in culture. Major secreted collagenous proteins of clonal myoblasts

Beach¹,

Rao²,

Festoff³

1985

Biochemical Journal

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We have previously shown that G8-1, a murine clonal skeletal-muscle cell line, produces a substrate-attached extracellular matrix [Beach, Burton, Hendricks & Festoff (1982) J. Biol. Chem. 257, 11437-11442]. To examine further the expression of extracellular-matrix proteins by muscle cells, we have analysed the collagenous proteins secreted by G8-1 myoblasts. We have found that collagens and/or procollagens, corresponding to genetic types I, III and IV (and possibly V), are produced and secreted by G8-J myoblasts. The major secreted collagenous polypeptides were identified as al type I and its precursors by using pulse-chase studies, pepsin and collagenase digestions and CNBr fragmentation. The presence of lesser amounts of the other collagens was determined by immunoprecipitation. These results demonstrate that clonal skeletal-muscle cells, in the absence of fibroblasts and an exogenous -collagen substrate, are able to synthesize and secrete several extracellular-matrix collagenous proteins in proportions similar to those which are commonly found in muscle tissue and mixed cultures of muscle cells and fibroblasts.

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Dose‐dependent initiation of myogenesis by neurotrophic factor

Cited by 13 publications

References 63 publications

Regulation of mitotic activity and the cell cycle in primary chick muscle cells by neurotransferrin

Regulation of mitotic activity and the cell cycle in primary chick muscle cells by neurotransferrin

Monoclonal antibody detects embryonic epitope specific for nerve‐derived transferrin

Extracellular-matrix synthesis by skeletal muscle in culture. Major secreted collagenous proteins of clonal myoblasts

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