Dose inseminante utilizada na fertilização artificial de ovócito de piracanjuba (Brycon orbignyanus)

Abstract

Insemination dose used in the artificial fertilization of piracanjuba ovocyte (Brycon orbignyanus)Piracanjuba (Brycon orbignyanus Valenciennes, 1849) is an migratory fish listed as an endangered species. The aim of this study was to determine the insemination dose for in vitro fertilization of piracanjuba oocytes. The experiments were arranged in a randomized block design with four treatments and three replications. Three piracanjuba couples selected from the breeding tanks at the Itutinga Environmental Station (IES-CEMIG), Minas Gerais State, Brazil, in the spawning period of 2006-2007, were applied crude extract of carp pituitary hormone for the production of gametes. Four different treatments were used for fertilization of 0.1 gram of oocytes: 10 μL, 20 μL, 30 μL and 40 μL of sperm. The samples were activated with 5 mL of water from the tank and then taken to a hatchery with constant water flow and temperature at 28 ºC. After 8 and 16 hours, the rates of fertilization (viable eggs) and hatching eggs were analyzed, respectively. The results were compared by Tukey test at 5% probability level. Tested sperm/oocyte ratios did not alter the rates of fertilization and hatching (P>0.05). The sperm/oocyte ratio ranging from 10.4 x10 5 to 41.6 x10 5 was efficient to achieve good fertility rates.

Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864)

“…In mammals, like cattle, horses and pigs, insemination doses are standardized with regards to semen volume and sperm concentration. However, in fish no ideal sperm concentration for the fertilization of a given oocyte is known (Bombardelli et al, 2006) for species such as dourado (Salminus brasiliensis) (Sanches et al, 2009), piracanjuba (Brycon orbignyanus) (Felizardo et al, 2010) and tambaqui (Colossoma macropomum) (Leite et al, 2013). The failure to use a minimum inseminating dose for species…”

Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator (Characiformes: Prochilodontidae)

“…Subsequent to exposure time (8 h), the embryos were transferred to commonly used 1L incubators (Felizardo et al, 2010) in a masonry tank with constant water circulation and oxygenation to complete their development. After hatching, the larvae were evaluated in a stereomicroscope, and the total number of viable larvae was counted (i.e.…”

Influential Factors of Quality of Fish Gametes for Use of In Vitro Fertilization

“…The sperm concentration assessment is traditionally used for in vitro fertilization. The sperm concentration presents the amount of sperm per ml of semen and can be determined by counting in volumetric chambers, by diluting semen in formolcitrate [22]. Concentration values may vary according to weight and age of the fish, season [13], collection frequency and ejaculation volume [6].…”