Dose of allergens in a peanut snack (Bamba) associated with prevention of peanut allergy

Hindley, James P.; Filep, Stephanie; Block, Denise; King, Eva-Maria; Chapman, Martin D.

doi:10.1016/j.jaci.2017.05.050

Cited by 14 publications

(6 citation statements)

References 11 publications

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“…In a companion article we have estimated the doses of Ara h 1, Ara h 2, and Ara h 6 present in a peanut snack (Bamba) that were associated with the prevention of peanut allergy. 3,27 The ability to compare specific antibody responses, cellular responses, and inflammatory markers with specific allergen concentrations in peanut vaccines will facilitate mechanistic studies of food allergy.…”

Section: Clinical Significance Of Variability In Peanut Allergen Levelsmentioning

confidence: 99%

Specific allergen profiles of peanut foods and diagnostic or therapeutic allergenic products

Filep

Block

Smith

et al. 2018

Journal of Allergy and Clinical Immunology

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The results show marked differences in specific peanut allergen profiles in peanut butter and flour and peanut preparations for clinical use. Roasting can increase Ara h 1 levels in peanut butter. Variability in allergen levels could affect the outcome of clinical trials of peanut OIT, especially with respect to Ara h 1. Specific allergen measurements will improve standardization and provide accurate dosing of peanut preparations that are being used for OIT.

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Section: Clinical Significance Of Variability In Peanut Allergen Levelsmentioning

confidence: 99%

Specific allergen profiles of peanut foods and diagnostic or therapeutic allergenic products

Filep

Block

Smith

et al. 2018

Journal of Allergy and Clinical Immunology

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“…Currently, whole peanut extract is the most commonly used reagent for skin prick test (SPT) and AIT study, which can potentially elicit adverse events including anaphylaxis [ 25 ]. Furthermore, the strategies of peanut immunotherapy have varied from subcutaneous, epicutaneous, and sublingual application to oral treatment but none of them are recommended for routine clinical usage because of the different dosing schedules and the varying duration of treatment [ 26 , 27 ]. Here, we established a new method to quantify Ara h 2 using purified mAbs against the allergen, which may help to standardize other immunotherapeutic reagents.…”

Section: Discussionmentioning

confidence: 99%

A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies

Chen

Zou

Tao

2018

Journal of Immunology Research

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Peanut (Arachis hypogaea) is one of the most common food allergens that can induce fatal anaphylaxis, and Ara h 2 is one of the major allergen components involved in peanut allergy. The aim of this study was to develop a quantitative method for detecting peanut allergen using monoclonal antibodies against Ara h 2. The splenocytes of immunized mice were fused with myeloma cells (SP2/0), and stable mAb-producing clones were obtained by limiting dilution. mAbs against Ara h 2 were isolated from mouse ascites, and specificity was confirmed by immunoblotting. Five mAbs with high purity and specific reactivity were obtained, which were referred to as 1-2E10, 2-1D5, 3-1C5, 4-1C2, and 5-1G4, respectively. After screening different mAb combinations for development of a sandwich ELISA, we selected 5-1G4 as the capture antibody and 1-2E10 as the detection antibody for the measurement of Ara h 2 from which an optimal correlation between the Ara h 2 concentration and the OD value was obtained. This sandwich ELISA could specifically detect Ara h 2 in peanut extract at concentrations as low as 5 ng/mL and up to 10 μg/mL. These mAbs can, therefore, serve as quantitative diagnostic reagents for peanut and peanut product risk assessment.

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“…Molecule‐based approaches are increasingly being applied to food allergy. Monoclonal antibody‐based ELISA for specific food allergens, including peanut (Ara h 1, Ara h 2, Ara h 3, Ara h 6); soy (Gly m 4, Gly m 8); egg (Gal d 2); milk (Bos d 5, Bos d 11) and carrot (Dau c 1, Dau c 4) have been developed and applied to measure allergens in foods, processed foods, immunotherapy products and early introduction foods 510–517 . A modified multiplex array, MARIA for Foods, measures up to 17 major food allergens.…”

Section: A12 – Molecular Exposure: Systematic Approaches Clinical Sig...mentioning

confidence: 99%

EAACIMolecular Allergology User's Guide 2.0

Dramburg

Hilger

Santos

et al. 2023

Pediatric Allergy Immunology

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119

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Since the discovery of immunoglobulin E (IgE) as a mediator of allergic diseases in 1967, our knowledge about the immunological mechanisms of IgE‐mediated allergies has remarkably increased. In addition to understanding the immune response and clinical symptoms, allergy diagnosis and management depend strongly on the precise identification of the elicitors of the IgE‐mediated allergic reaction. In the past four decades, innovations in bioscience and technology have facilitated the identification and production of well‐defined, highly pure molecules for component‐resolved diagnosis (CRD), allowing a personalized diagnosis and management of the allergic disease for individual patients. The first edition of the “EAACI Molecular Allergology User's Guide” (MAUG) in 2016 rapidly became a key reference for clinicians, scientists, and interested readers with a background in allergology, immunology, biology, and medicine. Nevertheless, the field of molecular allergology is moving fast, and after 6 years, a new EAACI Taskforce was established to provide an updated document. The Molecular Allergology User's Guide 2.0 summarizes state‐of‐the‐art information on allergen molecules, their clinical relevance, and their application in diagnostic algorithms for clinical practice. It is designed for both, clinicians and scientists, guiding health care professionals through the overwhelming list of different allergen molecules available for testing. Further, it provides diagnostic algorithms on the clinical relevance of allergenic molecules and gives an overview of their biology, the basic mechanisms of test formats, and the application of tests to measure allergen exposure.

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Dose of allergens in a peanut snack (Bamba) associated with prevention of peanut allergy

Cited by 14 publications

References 11 publications

Specific allergen profiles of peanut foods and diagnostic or therapeutic allergenic products

Specific allergen profiles of peanut foods and diagnostic or therapeutic allergenic products

A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies

EAACIMolecular Allergology User's Guide 2.0

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