Dose–Response Bioconversion and Toxicity Analysis of Magnetite Nanoparticles

Ruiz, Amalia; Mancebo, A.; Beola, Lilianne; Sosa, I.M.E. Garcia; Gutiérrez, Lucía

doi:10.1109/lmag.2016.2535414

Cited by 13 publications

(5 citation statements)

References 23 publications

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“…Simpler versions of this quantification procedure were used in the past for the analysis of the biodistribution of magnetic nanoparticles ,,,,− or the presence of ferritin in iron-overload animal models. ,, …”

Section: Resultsmentioning

confidence: 99%

Iron Speciation in Animal Tissues Using AC Magnetic Susceptibility Measurements: Quantification of Magnetic Nanoparticles, Ferritin, and Other Iron-Containing Species

Fernández‐Afonso

Asín

Beola

et al. 2022

ACS Appl. Bio Mater.

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The simultaneous detection and quantification of several iron-containing species in biological matrices is a challenging issue. Especially in the frame of studies using magnetic nanoparticles for biomedical applications, no gold-standard technique has been described yet and combinations of different techniques are generally used. In this work, AC magnetic susceptibility measurements are used to analyze different organs from an animal model that received a single intratumor administration of magnetic nanoparticles. The protocol used for the quantification of iron associated with the magnetic nanoparticles is carefully described, including the description of the preparation of several calibration standard samples of nanoparticle suspensions with different degrees of dipolar interactions. The details for the quantitative analysis of other endogenous iron-containing species such as ferritin or hemoglobin are also described. Among the advantages of this technique are that tissue sample preparation is minimal and that large amounts of tissue can be characterized each time (up to hundreds of milligrams). In addition, the very high specificity of the magnetic measurements allows for tracking of the nanoparticle transformations. Furthermore, the high sensitivity of the instrumentation results in very low limits of detection for some of the iron-containing species. Therefore, the presented technique is an extremely valuable tool to track iron oxide magnetic nanoparticles in samples of biological origin.

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Section: Resultsmentioning

confidence: 99%

Iron Speciation in Animal Tissues Using AC Magnetic Susceptibility Measurements: Quantification of Magnetic Nanoparticles, Ferritin, and Other Iron-Containing Species

Fernández‐Afonso

Asín

Beola

et al. 2022

ACS Appl. Bio Mater.

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“…1 Nevertheless, NPs with a size ranged of 20-50 nm are considered appropriate application involving drug delivery. 39 NPs with a size less than 20 nm (eg 10 nm) have been shown to accumulate in the liver and spleen of rats, 40 while NPs of larger size (20-100 nm) have been found to accumulate in the kidney. 41 On the other hand, NPs with a size less than 20 nm could be rapidly cleared by the kidneys, 42,43 while larger NiNPs may be difficult to be uptaken by cells.…”

Section: Discussionmentioning

confidence: 99%

<p>Internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys</p>

Abdulqadir¹,

Aziz²

2019

IJN

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Purpose: Since nanoparticles (NPs) are beginning to be introduced in medicine and industry, it is mendatory to evaluate their biological side-effects, among other things. The present study aimed to investigate the pathways by which nickel nanoparticles (NiNPs) enter nephrons and to evaluate their localization and effects on cellular ultrastructure. Methods: Rats were injected intraperitoneally with 20 nm NiNPs (20 mg/Kg/b.w./day) for 28 consecutive days. Transmission electron microscope technique was used to detect localization of NiNPs and their effects on cellular ultrastructure in rat kidneys. Additionally, measurements of certain biochemical parameters such as creatinine, urea, uric acid and phosphorus for investigating renal function following NiNPs treatment were taken. Results: The presence of NiNPs in the nephrons in treated rats was confirmed by transmission electron microscopy. NiNPs entered the renal tubules cells via various pathways. The results indicated that NiNPs administration induced ultrastructural changes in the proximal cells of renal tubules and certain glomerular cells (podocytes and mesangial cells). Additionally, NiNPs were found to be localized in the mitochondria, which led to a significant decrease in their density and morphology. Furthermore, cell death was induced in the glomerular cells as found with a Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) assay and through detection of p35 using immunohistochemical staining. Conclusion: Herein, NiNPs were found to induce various cellular ultrastructural changes in the kidneys of rats. NiNPs used diverse pathways to internalize into the cytoplasm of the proximal convoluted tubules (PT) cells across the basement membrane, and also through the plasma membrane of two adjacent PT cells. NiNPs internalization, accumulation and their alterations of the cellular ultrastructure affected rat renal function.

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“…It was indicated that below 30 ug/ml, MNPs did not impair cell viability or metabolic cell activity (van Buul et al, 2011). In vivo assays indicated the accumulation of MNPs in the liver and spleen, and found that the MNPs did not modify any of the toxicological parameters with the increase of MNPs dose (Sharifi et al, 2012;Li et al, 2013;Ruiz et al, 2016). Furthermore, Mejias et al (2013) observed the long-term toxicity of MNPs in vivo, and indicated that MNP did not compromise mouse survival, although acute toxicity were found 30 days after administration in some mouse, these were transient.…”

Section: Effects Of Mam On the Biological Behaviors Of Cellsmentioning

confidence: 99%

Magnetically Actuated Manipulation and Its Applications for Cartilage Defects: Characteristics and Advanced Therapeutic Strategies

Zhang

Cai

Lin

et al. 2020

Front. Cell Dev. Biol.

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For the fact that articular cartilage is a highly organized and avascular tissue, cartilage defects are limited to spontaneously heal, which would subsequently progress to osteoarthritis. Many methods have been developed to enhance the ability for cartilage regeneration, among which magnetically actuated manipulation has attracted interests due to its biocompatibility and non-invasive manipulation. Magnetically actuated manipulation that can be achieved by introducing magnetic nanoparticles and magnetic field. This review summarizes the cutting-edge research on the chondrogenic enhancements via magnetically actuated manipulation, including cell labeling, cell targeting, cell assembly, magnetic seeding and tissue engineering strategies.

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Dose–Response Bioconversion and Toxicity Analysis of Magnetite Nanoparticles

Cited by 13 publications

References 23 publications

Iron Speciation in Animal Tissues Using AC Magnetic Susceptibility Measurements: Quantification of Magnetic Nanoparticles, Ferritin, and Other Iron-Containing Species

Iron Speciation in Animal Tissues Using AC Magnetic Susceptibility Measurements: Quantification of Magnetic Nanoparticles, Ferritin, and Other Iron-Containing Species

<p>Internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys</p>

Magnetically Actuated Manipulation and Its Applications for Cartilage Defects: Characteristics and Advanced Therapeutic Strategies

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