Dose–response curves for analyzing of dicentric chromosomes and chromosome translocations following doses of 1000 mGy or less, based on irradiated peripheral blood samples from five healthy individuals

Abe, Yu; Yoshida, Mitsuaki A.; Fujioka, Kurumi; Kurosu, Yasuhiko; Ujiie, Risa; Yanagi, Aki; Tsuyama, Naohiro; Miura, Tomisato; Inaba, Toshiya; Kamiya, Kenji; Sakai, Akira

doi:10.1093/jrr/rrx052

Cited by 26 publications

(16 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, type E acentric fragments increased by a factor of 2.9 after 20 MRI sessions, equivalent to their tripling, very low in comparison with CT scans. Moreover, and interestingly, the profile of CA evolution in MRI-exposed volunteers was not the same as those usually observed after CT scans [22][23][24][25][26][27], as we mainly detected chromosomal deletions and no increase in Dics, the hallmark of irradiation. We hypothesise that the number of DSBs induced per cell by repeated MRIs is too low to induce chromosomal fusion.…”

Section: Discussioncontrasting

confidence: 52%

The effects of repeated brain MRI on chromosomal damage

et al. 2022

View full text Add to dashboard Cite

Background Magnetic resonance imaging (MRI) is currently considered a safe imaging technique because, unlike computed tomography, MRI does not expose patients to ionising radiation. However, conflicting literature reports possible genotoxic effects of MRI. We herein examine the chromosomal effects of repeated MRI scans by performing a longitudinal follow-up of chromosomal integrity in volunteers. Methods This ethically approved study was performed on 13 healthy volunteers (mean age 33 years) exposed to up to 26 3-T MRI sessions. The characterisation of chromosome damage in peripheral blood lymphocytes was performed using the gold-standard biodosimetry technique augmented with telomere and centromere staining. Results Cytogenetic analysis showed no detectable effect after a single MRI scan. However, repeated MRI sessions (from 10 to 20 scans) were associated with a small but significant increase in chromosomal breaks with the accumulation of cells with chromosomal terminal deletions with a coefficient of 9.5% (95% confidence interval 6.5–12.5%) per MRI (p < 0.001). Additional exposure did not result in any further increase. This plateauing of damage suggests lymphocyte turnover. Additionally, there was no significant induction of dicentric chromosomes, in contrast to what is observed following exposure to ionising radiation. Conclusions Our study showed that MRI can affect chromosomal integrity. However, the amount of damage per cell might be so low that no chromosomal rearrangement by fusion of two deoxyribonucleic breaks is induced, unlike that seen after exposure to computed tomography. This study confirms that MRI is a safe imaging technique.

show abstract

Section: Discussioncontrasting

confidence: 52%

The effects of repeated brain MRI on chromosomal damage

et al. 2022

View full text Add to dashboard Cite

show abstract

“…It is also important to record that dicentric aberrations are hardly induced by low radiation doses thus putting an additional constraint on the popular DCA method [38][39][40][41]. The di culty in estimating dose-response curves for low dose range was expressed and was concluded that DCA as well as translocation analysis after gamma irradiation particularly less than a 0.1 or 0.05 Gy was devoid of accuracy and showed poor dose-response [42]. However, accuracy could be improved by analyzing more than 5000 cells for the low dose range [43], which might be impractical in case of a radiation disaster situation.…”

Section: Discussionmentioning

confidence: 99%

Linear Dose Response of Acrocentric Chromosome Associations (Aca) To Gamma Irradiation In Human Lymphocytes

Samarth¹,

Gandhi²,

Chaudhury³

2021

Preprint

View full text Add to dashboard Cite

Purpose: The frequency of acrocentric chromosome associations (ACA) was studied to determine the possible dose-response relation with low doses of gamma irradiation in lymphocytes. Methods: Peripheral blood collected from three healthy donors were irradiated with 0, 0.1, 0.25, 0.5, 0.75, and 1 Gy gamma radiation. Chromosomal preparations were made after 48 hrs culture as per the standard guidelines. Results: The average number of ACA and ACA % were increased significantly with an increase in a dose. The D-G and D-D type of association was most prominent and showed a dose-dependent increase. The ACA frequency in irradiated lymphocytes showed an increase concerning the dose. The fitted regression equation was y=0.4759x+0.1663 (R2=0.9635; p=0.0005). An assessment of dicentric chromosomes (DC) was carried for the same slides. The correlation curve was prepared for ACA frequencies versus DC frequencies, resulting in a regression equation as y=8.659x+0.2.37 (R2=0.8275; p=0.0119). Conclusion: Our results showed an increase in frequencies of ACA in irradiated lymphocytes with an increase in radiation dose and followed a similar linear trend with DC frequency, thus, ACA may serve as a candidate cytogenetic biomarker for radiation biodosimetry especially for low radiation doses.

show abstract

“…Apparently stable complex exchanges (3 or more breaks in 2 or more chromosomes) were broken down into simple translocations and included in the total translocation count [19,20]. Genomic frequencies of stable chromosomal translocations (F G ) were calculated according to a standard formula proposed by Lucas and Sachs [21].…”

Section: Subjects (Participants)mentioning

confidence: 99%

Different chromosome damage in lymphocytes of newly diagnosed gastrointestinal and breast cancer patients

Kadlčíková¹,

Musilová²,

Hradská³

et al. 2020

neo

View full text Add to dashboard Cite

Structural chromosome aberrations are a predictive biomarker of cancer risk. Conventional chromosome analysis widely used for these purposes detects unstable chromosome aberrations that are eliminated during cell division. Stable aberrations that may persist in the body and tend to accumulate during a lifetime can be detected by fluorescence in situ hybridization (FISH). The aim of the study was to investigate the level of chromosome damage in newly diagnosed cancer patients and control subjects by FISH. Both groups of untreated cancer patients had increased frequency of aberrant cells. However, chromosome damage affected different cytogenetic endpoints. Stable translocations and cells with complex rearrangements were elevated in breast cancer patients whereas unstable chromosome aberrations (dicentric chromosomes and acentric fragments) were elevated in gastrointestinal cancer patients. These associations observed in nonsmokers were typically not pronounced in smokers (with the exception of dicentric chromosomes in gastrointestinal patients). Exposure to tobacco smoke increased aberrations in healthy controls but not in the cancer patients. Our study suggests an association between cancer and stable chromosomal rearrangements in breast cancer patients. Unstable aberrations elevated in gastrointestinal cancer patients may be at least partly ascribed to the exposure to diagnostic X-rays.

show abstract

Dose–response curves for analyzing of dicentric chromosomes and chromosome translocations following doses of 1000 mGy or less, based on irradiated peripheral blood samples from five healthy individuals

Cited by 26 publications

References 36 publications

The effects of repeated brain MRI on chromosomal damage

The effects of repeated brain MRI on chromosomal damage

Linear Dose Response of Acrocentric Chromosome Associations (Aca) To Gamma Irradiation In Human Lymphocytes

Different chromosome damage in lymphocytes of newly diagnosed gastrointestinal and breast cancer patients

Contact Info

Product

Resources

About