Dose-response effects of omega-3 on platelet aggregation: an observational study

Abstract

Objective This study aimed to evaluate the dose-response effects of supplemental omega-3 fatty acids on platelet function in healthy volunteers. Methods Twelve healthy volunteers ingested a normal supplemental dose of 1260 mg omega-3 fatty acids daily for 5 days, followed by a high dose of 2520 mg daily for another 5 days. Multiple electrode aggregometry (MEA) with four different agonists was used to measure platelet aggregation before and after the normal- and high-dose regimes. In vitro spiking using physiological doses of omega-3 fatty acids was also performed to determine whether MEA is capable of detecting a platelet-inhibiting effect due to omega-3 fatty acids. Results There were no differences in platelet aggregation measured by the MEA assay in healthy volunteers after intake of either the normal or high dose of omega-3 fatty acids. In the in vitro experiment, a platelet-inhibiting effect of omega-3 fatty acids was shown by an arachidonic acid agonist in MEA . Conclusions Supplemental omega-3 fatty acids do not evoke their positive health effects through inhibition of platelet aggregation measurable with MEA.

Synergistic platelet inhibition between Omega-3 and acetylsalicylic acid dose titration; an observational study

“…2). However, it must be stated that every individual value of ASPI AUC (without ASA) following Omega-3 intake was within normal MEA reference range [24,26,27], whereas some were below the reference value before Omega-3 intake (see below). Also, in the ASPI assays with both low and high dose ASA, all but 1 volunteer were below 30 U AUC (the exception having an AUC of 32 U in the ASPI+ASAx10 assay) following treatment.…”

“…ADP is an important agonist which induces platelet aggregation through the membrane-bound P2Y 12 -receptor by indirectly increasing the cytosolic calcium-level (Ca 2+ ) and thereby enable the complex formation of GpIIb/IIIa [45]. However, some studies show the contrary, indicating a negligible effect on ADP-induced platelet aggregation in the presence of Omega-3 fatty acids [27,46].…”

“…This change in electrical current is plotted in a graph over time and the results are expressed as area under the curve (AUC). The AUC represents the extent of platelet aggregation [26,27].…”

“…Sample size was calculated using G*Power version 3.1, (Heinrich-Heine-Universität Düsseldorf, Germany) and was based on previous data on MEA-analyses before and after Omega-3 intake [26,27]. With an alfa error probability of 0.05 and a power of 0.9 the sample size had to be 8.…”

Effect of yellow stripe scad (YSS) fish consumption on platelet microparticles markers: Can YYS fish be like salmon in overweight healthy individual?

“…Other effects of EPA/DHA include protection against CVD by reducing serum triacylglycerides (TAG) 79 , heart rate 80 , blood pressure (BP) 81 , and serum homocysteine levels 82 , as well as regulating and resolving inflammatory process 83 as shown in randomized trials with fish oil supplementation. However, other reports have shown that omega-3 have no effect on platelet aggregation 84 . Despite so, there is a paucity of available data on yellow stripe scad (YSS) as a source of omega-3 fatty acid in overweight healthy subjects.…”