Dose–response evaluation of a novel essential oil against Mutans streptococci in vivo

Abstract

Research has demonstrated the need for identifying a novel antimicrobial agent for topical use in the pediatric dental population. The essential oil of Lippia sidoides Cham. (LSO) has been described as having favorable biological properties, and a broad in vitro and in vivo antimicrobial spectrum against bacteria and yeast infections. Our aim was to determine a dose and formulation of LSO, acceptable for clinical testing in a pediatric population with dental caries. Thirty-seven 6-12-year old children were selected to participate in this study, and randomly allocated to receive different concentrations of either a gel (0.8%, 1%, 1.2% and 1.4%) or a mouth rinse (0.6%, 0.8%, 1% and 1.2%) formulation. The highest percentage MS reduction was observed with 0.8% mouth rinse and 1.4% gel. The efficacy of these concentrations was compared with a Thy-Car mixture formulated as a mouth rinse and gel treatments in 11 children. Saliva was collected after a single application of the antimicrobial treatment to establish effectiveness against MS. Both rinse (p<0.001) and gel (p=0.02) formulations produced significant MS reduction. Mouth rinse concentrations above 0.8% were associated with a transient intra-oral burning sensation. In conclusion, mouth rinse and gel LSO formulations demonstrated effectiveness against MS and good acceptance among children. We suggest future randomized clinical trials to test its effectiveness against early childhood caries.

Leishmanicidal activity of carvacrol-rich essential oil from Lippia sidoides Cham

“…is a perennial bushy plant native to the Caatinga, which is rich in aromatic essential oils. The medicinal properties of L. sidoides essential oil include antifungal (Fontenelle et al, 2007), antibacterial (Oliveira et al, 2006;Lobo et al, 2011), anti-helminthic (Camurça-Vasconcelos et al, 2008, larvicidal, and acaricidal activities (Carvalho et al, 2003;Cavalcanti et al, 2010). Some of these biological properties are attributed to the presence of thymol and carvacrol in its essential oil (Carvalho et al, 2003;Botelho et al, 2007).…”

Atividade Farmacológica do Óleo Essencial de Lippia sidoides em Odontologia: Uma Revisão de Literatura

“…Estudos in vivo utilizando formulações em gel, bochecho e dentifrício comprovaram através de parâmetros salivares a ação antibacteriana dos constituintes da Lippia sidoides, Timol e Carvacrol, na redução de Streptococcus mutans em crianças com cárie (LOBO et al, 2011).…”

“…De acordo com a OMS, plantas medicinais deveriam ser a melhor fonte de obter-se uma variedade de droga, pois o sistema público de saúde no Brasil não possui uma política de assistência farmacêutica capaz de suprir as necessidades medicamentosas da população, sobretudo no Nordeste brasileiro (LOBO et al, 2011;NASCIMENTO et al, 2007).…”

“…Diversas são as formulações medicamentosas de origem natural descritas para uso odontológico, onde destacam-se cimento endodôntico, vernizes dentários, dentifrícios e formulações de gel e bochecho de diversas plantas (LOBO et al, 2014;BARROSO, 2010;VASCONCELOS et al, 2008;BOTELHO et al, 2007b;BOTELHO et al, 2008 (LOBO et al, 2011).…”

Immunomodulatory Effects of Lippia sidoides Extract: Induction of IL-10 Through cAMP and p38 MAPK-Dependent Mechanisms

“…2,3 Essential oils extracted from its leaves have been shown to have antimicrobial properties for oral care, reducing gingivitis, and minimizing bone loss and inflammation associated with periodontal disease. 1,[4][5][6][7] Its action as an antileishmanial shows that L. sidoides may also be an effective alternative treatment for protozoal infections, 8,9 and the essential oil and hydrolysates have been shown to be effective pesticides against mollusks, 10 mosquito larvae, 11 and spider mites. 12 Elevation of intracellular cyclic adenosine monophosphate (cAMP) activates protein kinase A (PKA)-mediated phosphorylation and activation of cAMP response elementbinding protein (CREB).…”