Dose–response relationship for exercise on severity of experimental osteoarthritis in rats: a pilot study

Galois, Laurent; Étienne, S.; Grossin, Laurent; Pinzano, Astrid; Cournil, Christel; Lœuille, Damien; Netter, Patrick; Mainard, Didier; Gillet, Pierre

doi:10.1016/j.joca.2004.06.008

Cited by 134 publications

(145 citation statements)

References 40 publications

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“…8 In vivo, the beneficial influences produced by moderate exercise in rats subjected to ACLT were manifested by an overexpression of heat shock protein 70 and a decrease in chondrocyte apoptosis. 10 These factors can enhance the defense mechanisms acting against oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…10,12,28 In the standpoint of biomarkers, the running protocol which we set 18 m/min, 30 min/day, three times per 5); (h) medial part of (e) (Â100); (i) lateral part of (f) (Â40); (j) medial part of (g) (Â100).…”

Section: Discussionmentioning

confidence: 99%

“…7,8 Moderate mechanical loading maintains the integrity of articular cartilage, but both disuse and overuse can result in cartilage degradation. [9][10][11][12][13] Forced mobilization accelerated OA damage in rats subjected to anterior cruciate ligament transaction (ACLT). 9 In another report, rats made to exercise following ACLT developed more cartilaginous lesions than did controls with no exercise, but the lesions in the control rats were usually as severe as those in the exercising rats.…”

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confidence: 99%

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Effects of exercise level on biomarkers in a rat knee model of osteoarthritis

Yamaguchi

Aoyama

Ito

et al. 2013

Journal Orthopaedic Research

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The aim of this study was to elucidate whether the levels of serum biomarkers reflect the progression of osteoarthritis (OA) induced by different levels of exercise. Thirty-five Wistar rats subjected to anterior cruciate ligament transaction (ACLT) were divided into three groups: Control, moderate running, and intense running. Twelve rats (moderate running without ACLT) were allocated as a naive group. Running was performed on a motorized treadmill, at a speed of 18 m/min for 30 min/day (moderate and naive) or 60 min/day (intense) for 3 days per week. After 2 or 4 weeks, OA histopathology in the knees was evaluated using the Osteoarthritis Research Society International (OARSI) score, and the serum levels of cleaved collagen type II (C2C) and procollagen II C-propeptide (CPII) were analyzed. The OARSI score deteriorated in the intense running group after 2 weeks and the serum C2C/CPII ratio suggested the development of OA. At 4 weeks, the C2C/CPII ratio suggested there would be deterioration in the OARSI score but the score did not differ significantly between the moderate and intense running groups. C2C/CPII ratio had 13-25% correlation with the OARSI histological score. Thus, in rat experimental OA, the OARSI score could be partially predicted by the C2C/CPII ratio as a serum biomarker of OA. Keywords: cartilage metabolism; osteoarthritis; running exercise; rats; biomarker Osteoarthritis (OA) of the knee, a common joint disorder among elderly people, is caused by aging, overuse, trauma, and other complicating factors, and leads to degenerative structural changes. In daily life, exercise is encouraged for individuals with poor locomotion caused by OA, but excessive stress can induce cartilage degeneration.1,2 The anterior cruciate ligament contributes to dynamic stability of the knee joint, and injury to this structure is a crucial factor leading to OA of the knee in human subjects, 3 rats, 4 and many other species. 5,6 Clinically, OA of the knee is treated using physical therapies such as stretching, muscle training, gait control, and some pharmacotherapeutic methods. 7,8 Moderate mechanical loading maintains the integrity of articular cartilage, but both disuse and overuse can result in cartilage degradation.9-13 Forced mobilization accelerated OA damage in rats subjected to anterior cruciate ligament transaction (ACLT). 9 In another report, rats made to exercise following ACLT developed more cartilaginous lesions than did controls with no exercise, but the lesions in the control rats were usually as severe as those in the exercising rats. 4 From a structural standpoint, articular cartilage is composed of chondrocytes and an extracellular matrix rich in molecules such as type II collagen and aggrecan. In individuals with OA, these components are degraded by various enzymes such as a disintegrin metalloproteinase with thrombospondin motifs (ADAMTS) and matrix metalloproteinases (MMPs).5,14-16 Cartilage degradation can be attributed to an imbalance between the anabolic and catabolic activities of chon...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of exercise level on biomarkers in a rat knee model of osteoarthritis

Yamaguchi

Aoyama

Ito

et al. 2013

Journal Orthopaedic Research

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“…High-intensity exercise has been associated with accelerated OA progression in several animal models (21)(22)(23). However, it has been found that mild to moderate exercise is protective against cartilage damage in the rat ACLT model (21).…”

Section: Acceleration Of Oa Progressionmentioning

confidence: 99%

Animal Models of Osteoarthritis: Challenges of Model Selection and Analysis

Teeple

Jay

Elsaid

et al. 2013

AAPS J

175

192

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ABSTRACT. Osteoarthritis (OA) is the most common musculoskeletal disease, affecting millions of individuals worldwide. New treatment approaches require an understanding of the pathophysiology of OA and its biomechanical, inflammatory, genetic, and environmental risk factors. The purpose of animal models of OA is to reproduce the pattern and progression of degenerative damage in a controlled fashion, so that opportunities to monitor and modulate symptoms and disease progression can be identified and new therapies developed. This review discusses the features, strengths, and weaknesses of the common animal models of OA; considerations to be taken when choosing a method for experimental induction of joint degeneration; and the challenges of measuring of OA progression and symptoms in these models.

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“…3 HSP70 promotes proteoglycan core protein production in chondrocytes 4 and suppresses chondrocyte apoptosis. 5 In a study employing a rat OA model, Galois et al, 6 found that mild to moderate exercise stress caused excess HSP70 expression in the chondrocyte and suppressed chondrocyte apoptosis. We believe that if HSP70 does indeed protect chondrocytes and could be appropriately expressed in these cells, the protein could be used to inhibit OA progression.…”

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confidence: 99%

Combined microwave irradiation and intraarticular glutamine administration‐induced HSP70 expression therapy prevents cartilage degradation in a rat osteoarthritis model

Fujita

Nakagawa

Takahashi

et al. 2011

Journal Orthopaedic Research

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The objective of the present study was to investigate the effects of heat stimulation and glutamine (Gln) on the expression of extracellular matrix genes and heat shock protein 70 (HSP70) in rat articular cartilage in vivo and to determine whether HSP70 expression achieved with a combination of microwave (MW) and Gln suppresses osteoarthritis (OA) progression in a rat OA model. Stimulation at 40 W was assumed to be appropriate in the present study, and the effects of heat treatment at this intensity were evaluated. Articular cartilage was collected at 8 h after heat stimulation and/or intraarticular Gln administration, and total RNA was extracted. The expression of HSP70, aggrecan, and type II collagen was quantified using real-time RT-PCR. Cartilage samples from the OA model were subjected to hematoxylin and eosin (HE) and safranin O staining. HSP70 and aggrecan expression was greatest in a group receiving both MW and Gln. In the rat OA model, the severity of OA was significantly milder in a group receiving MW and Gln than in the control group. HSP70, stimulated by the combination of MW heat and Gln, may be involved in the suppression of OA progression. ß

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Dose–response relationship for exercise on severity of experimental osteoarthritis in rats: a pilot study

Cited by 134 publications

References 40 publications

Effects of exercise level on biomarkers in a rat knee model of osteoarthritis

Effects of exercise level on biomarkers in a rat knee model of osteoarthritis

Animal Models of Osteoarthritis: Challenges of Model Selection and Analysis

Combined microwave irradiation and intraarticular glutamine administration‐induced HSP70 expression therapy prevents cartilage degradation in a rat osteoarthritis model

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