Dot Blot Immunoassay for Detection of Human Semen

Abstract

A sensitive and specific dot blot immunoassay based on prostate specific antigen (PSA) antibodies and a radiolabelled Protein A detection system was developed for detection of trace amounts of human semen in stains. The method was found highly sensitive and detected semen stain extracts diluted 10,000 times. Semen stains up to five years old were successfully detected by the method. The assay is found to be highly suitable for semen detection in forensic analysis.

Presumptive and Confirmatory Tests Used in Identification of Biological Evidence and the Latest Developments in This Topic

“…Sperm tespiti için diğer kullanımı olan metotlar; immünoelektroforez, ELISA ve radyolojik olarak işaretli Protein A içeren immunoassay teknikleri ya da membran aspirasyon testi olarak bilinen metotlardır. 39,40 Son yıllarda antijen-antikor reaksiyonuna dayalı olan çok daha hızlı ve kullanımı kolay olan test kitleri kullanıma sunulmuştur. Bu kitler geleneksel ELISA metotlarına göre daha ucuzdur ve ticari olarak en çok kullanılanları RSID ™ -Semen ve ABAcard test kitleridir.…”

Biochemical characterization of mammalian high mobility group protein A2

“…In 1989, an antigen known as prostate specific antigen (PSA) was first used to aid in the detection of seminal fluid stains (Kamenev et aL., 1989). An immunoassay was developed in 1992 for anti-PSA antigens using a radio-labeled Protein A detection system (Rao and Kashyap, 1992).…”

“…This technique is extremely sensitive and can detect stains up to five years old (Rao and Kashyap, 1992). In 2002 a "SMITEST" PSA immunochromatographic membrane test card was devised for ease of use to be utilized at the scene of a crime (Sato et al, 2001).…”