DOT-ELISA and parasitological examination for diagnosis of Schistosoma mansoni infection in Nigeria

Mafuyai, H.B.; Uneke, Chigozie Jesse; Njoku, M O; Chuga, G.

doi:10.2478/s11687-006-0003-3

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…The fi ndings of the current study also showed that participants aged 20 -40 years were signifi cantly more prone to be infected than younger and older ones. This result was in agreement with previous reports worldwide ( Raja'a et al, 2000;Gryseels et al, 2006;Mafuyai et al, 2006;Matthys et al, 2007;Ugbomoiko et al, 2010;Deribe et al, 2011). This could be explained by the excessive mobility of people at this age and they may become more exposed to infected water while swimming/playing or fetching water for domestic purposes or helping in agriculture activities.…”

Section: Discussionsupporting

confidence: 83%

Some epidemiological and serological studies on schistosomiasis in Najran area, Saudi Arabia

Elshahawy¹,

Mohamed

Adail

2016

Helminthologia

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SummarySchistosomiasis is a major cause of morbidity and mortality and has been estimated to infect over 200 million people. Diagnosis of schistosomiasis by detection of specifi c antibodies is likely to be more sensitive than the traditional method of diagnosis by detection of eggs in stool or urine. Therefore the present study was the fi rst attempt to highlight the seroepidemiology of schistosomiasis among the general population of Najran City, southern of Saudi Arabia, as well as to achieve the performance of the diagnostic tests used. A total of 180 participants attending King Khaled hospital in Najran Province, Saudi Arabia, over a one year period, from September 2013 to September 2014 were screened for the presence of Schistosoma antibody in their blood serum using an indirect hemagglutination assay (IHA). Specifi c immunoglobulin (Ig) G antibody was evaluated using an enzyme-linked immunosorbent assay (ELISA). Out of the 180 samples of sera tested using IHA, 32 (20 %) were found to be positive with a titer ranging from 1:160 to 1:1280, while 42(23.2 %) revealed Schistosoma IgG. A positive relationship was found between the seroprevalence of schistosomiasis and age of tested participants, especially in the age group of 20-40 years old. Additionally, prevalence of infection was more in males (36 %) than females (7.5 %), and showed statistical significance (P < 0.001). Similarly, there was signifi cant association between the presence of Schistosoma antibodies and the nationality of residence, and education of participants (P < 0.05). The current investigation reveals an alarmingly high prevalence of schistosomiasis among participants in Najran, southern region of Saudi Arabia and this supports an urgent need to re-evaluate the current control measures and implement an integrated, targeted and effective schistosomiasis control measures.

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Section: Discussionsupporting

confidence: 83%

Some epidemiological and serological studies on schistosomiasis in Najran area, Saudi Arabia

Elshahawy¹,

Mohamed

Adail

2016

Helminthologia

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“…Similarly, [52] observed higher frequency of contact in young children who indulged in watering of cattle, washing and bathing. In contrast, [7,53,54] observed that the 20-30year olds had more contact while washing, practicing irrigation farming, fetching water for daily routine household chores, fermentation of cassava tubers or even recreation.…”

Section: Discussionmentioning

confidence: 99%

“…In Africa alone, about 90% of the 207 million infected cases have been reported while in Nigeria, up to 101 million persons are at risk of schistosomiasis with nearly 29 million infected in 2008 [6,7,8]. Many people suffer from schistosomiasis, which can cause hypertension, seizures, urinary obstruction, organ damage or destruction and death.…”

Section: Introductionmentioning

confidence: 99%

Urogenital schistosomiasis in females from some suburban communities of Jos, north central Nigeria

Jc¹,

Ja²,

Sl³

et al. 2014

IOSRJPBS

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Comparative analysis of the diagnostic performance of adult, cercarial and egg antigens assessed by ELISA, in the diagnosis of chronic human Schistosoma mansoni infection

2014

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Total IgG ELISA was assessed comparing soluble adult worm (SWA), cercarial (SCA), and egg antigens (SEA) using sera of chronic schistosomiasis patients in two different concentrations (1/50 and 1/100). This response showed reactivity against all antigens. Concerning 1/100 serum concentration the SWA gave the best sensitivity (100%) followed by SCA (86.6%) and SEA (80%). The best specificity was obtained from SCA (94.11%) with an equivalent result from the SWA and SEA (76.47%). Concerning the 1/50 serum concentration, the SWA gave the best sensitivity (100%) with an equivalent result from the SCA and SEA (80%). The best specificity was obtained from SEA (82.35%) followed by SCA (76.47%) then SWA (64.7%). The best AUC value (0.992) was that for SWA indicating its high ability to predict the disease in comparison to (0.914) for SCA and 0.871 for SEA in serum dilution 1/100. The highest AUC value (0.955) was that for SWA in comparison to (0.914) for SEA and (0.88) for SCA in serum dilution 1/50. Comparison between the AUCs from the three mentioned antigens revealed highly significant differences when the data were analyzed by ROC. Each antigen showed a highly statistically significant correlation within its optical density values at both concentrations; 1/100 and 1/50. Comparing the two concentrations in each antigen revealed a non significant correlation obtained from the SWA which signifies a near outcome from both concentrations while the SCA and SEA showed a highly significant difference between the two concentrations. In conclusion, for the diagnosis of chronic schistosomiasis mansoni, total IgG reactivity revealed the best sensitivity by SWA. There was a difference in the type of antigen showing best specificity results between SCA and SEA according to serum concentration used.

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DOT-ELISA and parasitological examination for diagnosis of Schistosoma mansoni infection in Nigeria

Cited by 4 publications

References 18 publications

Some epidemiological and serological studies on schistosomiasis in Najran area, Saudi Arabia

Some epidemiological and serological studies on schistosomiasis in Najran area, Saudi Arabia

Urogenital schistosomiasis in females from some suburban communities of Jos, north central Nigeria

Comparative analysis of the diagnostic performance of adult, cercarial and egg antigens assessed by ELISA, in the diagnosis of chronic human Schistosoma mansoni infection

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