Double-antibody homogeneous fluorescence immunoassay of phenytoin

O'Neal, Jeffrey S.; Teague, Perry O.

doi:10.1016/s0003-2670(00)81737-7

Cited by 4 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has also provided a convenient method to study the changes in antibody affinities during the immune response and under varying immunization conditions [6]. Some early papers focused on the use of antibodies with fluorescent probes in the development of homogeneous immunoassays [7,8]. Examples of haptens which undergo fluorescent quenching on antibody binding are fluorescein [2] and anthracycline antibiotics [3].…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of a polyclonal antibody for Os(II) and Ru(II) polypyridyl complexes

O'Connor

Killard

O’Kennedy

et al. 2006

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

The characterization of a polyclonal antibody produced via immunization with an [Os(bpy)(2)dcbpy] hapten is described. Bpy is 2,2'-bipyridine and dcbpy is 2,2'-bipyridine-4,4'-dicarboxylic acid. The cross-reactivity of the antibody for the Ru(II) analogue of the hapten was also investigated. Large increases in the emission and luminescent lifetime of a series of Os and Ru complexes were observed on binding of the antibody. Association equilibrium constants were derived from luminescence titration data and were found to be 5.6 x 10(8) and 5.0 x 10(8)M(-1) for [Os(bpy)(2)dcbpy] and [Ru(bpy)(2)dcbpy], respectively. Spectroscopic changes were likely due to the exclusion of H(2)O from the complex/antibody binding cleft and blocking of vibrational relaxation pathways of the Os/Ru excited state. D(2)O/H(2)O experiments confirmed that the antibody protected approx. 82% of [Os(bpy)(2)dcbpy] and 80% of [Ru(bpy)(2)dcbpy] from excited state deactivation by the aqueous solvent.

show abstract

Section: Introductionmentioning

confidence: 99%

Production and characterization of a polyclonal antibody for Os(II) and Ru(II) polypyridyl complexes

O'Connor

Killard

O’Kennedy

et al. 2006

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

show abstract

“…Various analytical techniques are available for the determination of phenytoin, such as spectrophotometry, 2 HPLC, 3 titrimetry, 4 capillary electrophoresis (CE), 5 gravimetry, 6 ionselective electrodes (ISEs) 7 and immunoassay. 8 The spectrophotometric method requires certain reactions because of the significant overlap of the spectra. HPLC requires a tedious and time-consuming preliminary sample clean-up procedure.…”

Section: Introductionmentioning

confidence: 99%

Construction and application of phenytoin anion-selective electrode based on bulk acoustic wave (BAW) sensing technique

Long

Liu

Lei

et al. 2001

Analyst

View full text Add to dashboard Cite

This paper describes the construction of an anion-selective bulk acoustic wave (BAW) sensor for the direct determination of phenytoin sodium for the first time. Based on the sensitive mass response of a piezoelectric quartz crystal (PQC) and selective adsorption-desorption across the modified film, the BAW sensor was fabricated by coating a polyvinylchloride (PVC) film containing activator on one side of a PQC. The method was found to be sensitive, rapid and easy to handle without pretreatment of the sample. The logarithm of the frequency shift of the PQC shows a linear relationship to the logarithm of the concentration of phenytoin over the range 6.7 x 10(-8)-8.0 x 10(-4) M with a detection limit of 1.0 x 10(-8) M at pH 10.0. Recoveries were in the range 98.5-102.0%. Influencing factors were examined and optimized. Also discussed is the preliminary application of the phenytoin BAW sensor in serum and injection. The results for real samples obtained by the proposed method agreed with those obtained by conventional methods.

show abstract

“…A ligação de anticorpo a moléculas luminescentes pode resultar em diminuição ou aumento de fluorescência e, portanto, constitui-se um método sensível para avaliar sítios de ligação anticorpo-antigeno (WATT, 1977;CHIEN, 1975;PARKER et al, 1967;IWAKI et al, 1993). Além disto provém um método conveniente para avaliar o uso de diferentes anticorpos no processo relacionado à resposta imune (NARGESSI, 1979;O'NEAL,1985).…”

Section: Anticorpo Como Molécula Alvounclassified

Interação anticorpo policlonal-complexo de rutênio como sistemas de liberação de óxido nítrico. Medida da especificidade e avaliação citotóxica

Ramos¹

View full text Add to dashboard Cite

Double-antibody homogeneous fluorescence immunoassay of phenytoin

Cited by 4 publications

References 15 publications

Production and characterization of a polyclonal antibody for Os(II) and Ru(II) polypyridyl complexes

Production and characterization of a polyclonal antibody for Os(II) and Ru(II) polypyridyl complexes

Construction and application of phenytoin anion-selective electrode based on bulk acoustic wave (BAW) sensing technique

Interação anticorpo policlonal-complexo de rutênio como sistemas de liberação de óxido nítrico. Medida da especificidade e avaliação citotóxica

Contact Info

Product

Resources

About