Double Edge

Abstract

Cancer research has been devoted toward an understanding of the molecular regulation and functional significance of cell-cycle regulators in the pathogenesis and development of cancers. Cyclin-dependent Kinase 2-associated Protein 1 (CDK2AP1) is one such cell-cycle regulator, originally identified as a growth suppressor and a prognostic marker for human oral/head and neck cancers. Functional importance and the molecular mechanism of CDK2AP1-mediated cell-cycle regulation have been documented over the years. Recent progress has shown that CDK2AP1 is a competency factor in embryonic stem cell differentiation. Deletion of CDK2AP1 leads to early embryonic lethality, potentially through altered differentiation capability of embryonic stem cells. More intriguingly, CDK2AP1 exerts its effect on stem cell maintenance/differentiation through epigenetic regulation. Cancer cells and stem cells share common cellular characteristics, most prominently in maintaining high proliferative potential through an unconventional cell-cycle regulatory mechanism. Cross-talk between cellular processes and molecular signaling pathways is frequent in any biological system. Currently, it remains largely elusive how cell-cycle regulation is mechanistically linked to epigenetic control. Understanding the molecular mechanism underlying CDK2AP1-mediated cell-cycle regulation and epigenetic control will set an example for establishing a novel and effective molecular link between these two important regulatory mechanisms.

Dysregulated expression of microRNAs and mRNAs in pulmonary artery remodeling in ascites syndrome in broiler chickens

“…And in this study, we found more than one miRNA, including miR-142-5p, miR-32-5p, miR-1662, miR-155, miR-30e-5p and miR-34a-5p, that function together to target LRP1B in the pulmonary arteries of AS broilers, which was characterized by vascular cells proliferation. CDK2AP1 was shown to play a role in epithelial-to-mesenchymal transition, determining both cell fate and differentiation [42]. Significant upregulation of miR-146b-5p and its corresponding downregulation of CDK2AP1 were observed in the AS pulmonary arteries in our findings, which is consistent with Zhong et al, who reported that significant upregulation of miR-205 targeted the downregulation of CDK2AP1 in the laryngeal squamous cell carcinoma [43].…”

Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells

“…The migration profile of cell cycle regulator Cdk2ap1 ( 46 ) clustered closely with those of NuRD subunits ( Fig. 2 ).…”

“…B , Migration profiles for core NuRD subunits Chd4, Gatad2a/b, and Rbbp4/7 across the BN-PAGE separation length. Only fractions from the range of interest ( 32 – 46 ) in a representative experiment are displayed. C , Linear regression plot of normalized iBAQ values relative to Mta2 for Mta1/2/3, Hdac1/2, and Mbd3 outlining their similarity to the migration profile of Mta2.…”

Methylation Levels of SLC23A2 and NCOR2 Genes Correlate with Spinal Muscular Atrophy Severity

“…It was demonstrated that mSin3A is associated with the SMN protein [ 39 ]. The CDK2AP1 gene product, CDK2AP1 protein, is a subunit of the NuRD (Nucleosome Remodeling Deacetylase) complex, contained the histone deacetylases HDAC1, HDAC2 and the methyl-CpG-binding domain proteins MBD2 or MBD3 [ 40 ]. In the light of SMA interactions between SMRT and CDK2AP1 with histone deacetylases seems to be interesting, taking in the account that the main agents tested for SMA therapy are histone deacetylase inhibitors [ 41 ], [ 42 ].…”