Double-Enzymes-Mediated Bioluminescent Sensor for Quantitative and Ultrasensitive Point-of-Care Testing

Chen, Yiping; Xianyu, Yunlei; Wu, Jing; Dong, Mingling; Zheng, Wenshu; Sun, Jiashu; Jiang, Xingyu

doi:10.1021/acs.analchem.7b00239

Cited by 73 publications

(42 citation statements)

References 37 publications

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“…Significant efforts have been devoted to the development of rapid quantitative readouts in POCT assays, such as optical detection [21][22][23][24][25] , electrochemical detection [26][27][28][29] , and commercial portable instrument-based detection [30][31][32][33] . While these methods are sensitive and user-friendly, most of them are unsuitable for application with the broad population.…”

Section: Introductionmentioning

confidence: 99%

Naked-eye point-of-care testing platform based on a pH-responsive superwetting surface: toward the non-invasive detection of glucose

et al. 2018

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Herein, we demonstrate a contact angle (CA)-based naked-eye point-of-care testing platform with rapid pH-responsive superwettability that can switch between superhydrophilic and superhydrophobic properties for quantitative biosensing. The CA of the droplet on the pH-responsive surface approached~0°at pH 1 and conversely reached 161.4°± 6.2°at pH 13. We realized the sensitive detection of the pH, urea, and glucose by monitoring the changes in the CA. The traditional invasive diagnosis of diabetes causes pain and brings the risk of infections, such as acquired immune deficiency syndrome (AIDS), hepatitis B, hepatitis C, and syphilis, to the user. To address this issue, we implemented a method for the non-invasive diagnosis of diabetes in human saliva and urine, which avoided the significant drawbacks mentioned above. The accuracy of this method was demonstrated by comparing the results with those from commercial glucometers and theoretical calculations. Interestingly, we successfully monitored glucose levels in sweat before, during, and after cycling. The sensing performance was barely influenced by the temperature, elevation, and droplet color, illustrating promise for expansion to hundreds of millions of potential customers, especially those with color blindness or color weakness. Given its low cost, lack of instruments, and rapid response (within 1 s), this strategy might overcome the limitations of the mechanical stability and durability of superwettable materials and thus might extend the industrial-scale application of bioinspired superwettable systems.

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Section: Introductionmentioning

confidence: 99%

Naked-eye point-of-care testing platform based on a pH-responsive superwetting surface: toward the non-invasive detection of glucose

et al. 2018

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“…The introduction of nanomaterials into the assay enhances the signal and can even simplify the assay procedure. Magnetic nanoparticles allow ultrasensitive protein detection [196] with LODs that are 100 times lower than ELISA [197] (Figure 10c,d). Chen's group conjugated a recombinant protein that contained Renilla luciferase with AuNPs to fabricate a thrombin sensor that allowed sensitive detection (LOD: 80 pM) in urine within a short time (10 min) [198].…”

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

“…Furimazine systems have also been employed for intracellular ATP sensing [206], voltage indication in live cells [207] as well as metal ion detection and bioimaging [207][208][209]. [186]; (b) Vancomycin conjugated magnetic particles for gram-positive bacteria detection [187]; (c) Magnetic nanoliposomes for bioluminescent protein sensing [196]; (d) Magnetic sandwich assay for procalcitonin detection [197]; (e) Furimazine-based antibody detection using LUMABS [202]; (f) Obelin as a photoprotein in the sensing of anti-myelin basic protein autoantibody [210]. [186,187,196,197,202,210].…”

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

“…The sensor allowed bacteria detection down to 50 CFU mL −1 within 7 seconds [215]. Krasitskaya's group recently developed a dual-aptamer solid-phase sandwich-type microassay in serum for the ultrasensitive detection (LOD: 6.3 nM) of autoantibodies to myelin basic protein, a [186]; (b) Vancomycin conjugated magnetic particles for gram-positive bacteria detection [187]; (c) Magnetic nanoliposomes for bioluminescent protein sensing [196]; (d) Magnetic sandwich assay for procalcitonin detection [197]; (e) Furimazine-based antibody detection using LUMABS [202]; (f) Obelin as a photoprotein in the sensing of anti-myelin basic protein autoantibody [210]. Abbreviations: Ab: Antibody; Ala: Alanine; ALP: Alkaline phosphatase; AMP: Adenosine monophosphate; ATP: Adenosine triphosphate; B 0 /B 1 : Bioluminescence intensity; DPPC: 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine; DPPE: 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine; IgG: Immunoglobulin G; MBP: Myelin basic protein; MNP: Magnetic nanoparticles; PMT: Photomultiplier tube; PS: Carboxyl modified polystyrene microsphere; Obe: Obelin.…”

Section: Bioluminescence-based Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

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Recent Progress in Optical Sensors for Biomedical Diagnostics

2020

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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“…Different types of biosensor detection systems can be used for the determination of pollutants in water samples; however, the most suitable are optical‐based biosensors . Optical‐based biosensors feature comparatively high sensitivity and provide real‐time qualitative analysis without extensive sample preparation.…”

Section: Introductionmentioning

confidence: 99%

Disposable luciferase‐based microfluidic chip for rapid assay of water pollution

et al. 2018

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In the present study, we demonstrate the use of a disposable luciferase-based microfluidic bioassay chip for environmental monitoring and methods for fabrication. The designed microfluidic system includes a chamber with immobilized enzymes of bioluminescent bacteria Photobacterium leiognathi and Vibrio fischeri and their substrates, which dissolve after the introduction of the water sample and thus activate bioluminescent reactions. Limits of detection for copper (II) sulfate, 1,3-dihydroxybenzene and 1,4-benzoquinone for the proposed microfluidic biosensor measured 3 μM, 15 mM, and 2 μM respectively, and these values are higher or close to the level of conventional environmental biosensors based on lyophilized bacteria. Approaches for entrapment of enzymes on poly(methyl methacrylate) (PMMA) plates using a gelatin scaffold and solvent bonding of PMMA chip plates under room temperature were suggested. The proposed microfluidic system may be used with some available luminometers and future portable luminescence readers.

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Double-Enzymes-Mediated Bioluminescent Sensor for Quantitative and Ultrasensitive Point-of-Care Testing

Cited by 73 publications

References 37 publications

Naked-eye point-of-care testing platform based on a pH-responsive superwetting surface: toward the non-invasive detection of glucose

Naked-eye point-of-care testing platform based on a pH-responsive superwetting surface: toward the non-invasive detection of glucose

Recent Progress in Optical Sensors for Biomedical Diagnostics

Disposable luciferase‐based microfluidic chip for rapid assay of water pollution

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