Double Esterase Staining andOther Neutrophilic Granule Abnormalities in 237 Patients with the Myelodysplastic Syndrome Studied by the Cancer and Leukemia Group B

Elghetany, M. Tarek; Peterson, Bercedis L.; MacCallum, Jane; Nelson, Douglas A.; Varney, Judy F.; Sullivan, Arthur K.; Schiffer, Charles A.; Davey, FR; Bloomfield, Clara D.

doi:10.1159/000040855

Cited by 8 publications

(6 citation statements)

References 8 publications

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“…Normal bone marrow contains fewer than 3% of cells expressing a DE‐staining pattern ( Scott et al . 1983 ; Elghetany et al . 1998 ).…”

Section: Discussionsupporting

confidence: 88%

“…Alpha‐naphthyl acetate esterase and naphthol ASD chloroacetate esterase were used as substrates as previously described ( Hayhoe & Quaglino 1994). Fewer than 3% of all nucleated marrow cells expressed DE staining, which was consistent with a prior study ( Elghetany et al . 1998 ).…”

Section: Case Reportmentioning

confidence: 99%

“…A previously reported indirect evidence of myeloid differentiation of blasts includes the presence of MPO‐deficient neutrophils, a finding that has not been reported in acute lymphoblastic leukaemia (ALL) ( Bendix‐Hansen & Nielsen 1983a). In the author’s experience, an increased percentage of mature and maturing bone marrow (BM) cells that stain simultaneously for specific and non‐specific esterase (double esterase (DE)) is another specific indicator of myeloid differentiation ( Elghetany, MacCallum & Davey 1990; Elghetany et al . 1998 ).…”

Section: Introductionmentioning

confidence: 99%

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Case Report

Elghetany

1999

Clinical &Amp; Laboratory Haematology

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Minimally differentiated acute myeloid leukaemia (AML-M0) may pose difficulty in diagnosis since morphological criteria alone are not reliable. Other studies such as electron microscopy, immunocytochemistry and surface marker analysis are needed. The role of cytochemistry has been limited to the negative staining of blasts for Sudan Black B and myeloperoxidase. An abnormal morphology and cytochemistry of bone marrow maturing cells may indicate the myeloid nature of acute leukaemia. In this case of AML-M0, increased numbers of maturing and mature bone marrow granulocytic cells staining simultaneously for both specific and non-specific esterase (double esterase) are described. In acute leukaemia, this abnormal cytochemical finding seems to be specific for myeloid leukaemia and may be used as supplementary evidence of myeloid differentiation of morphologically undifferentiated blasts in cases of AML-M0.

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“…Normal bone marrow contains fewer than 3% of cells expressing a DE‐staining pattern ( Scott et al . 1983 ; Elghetany et al . 1998 ).…”

Section: Discussionsupporting

confidence: 88%

Section: Case Reportmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Case Report

Elghetany

1999

Clinical &Amp; Laboratory Haematology

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“…In addition, MDS may evolve from an existing acquired AA, particularly in patients receiving prolonged treatment with G-CSF [23]. A thorough morphological assessment complemented by cytochemical, immunocytochemical, and cytogenetic studies may provide tools for making such a distinction [9,10,12,26]. Considering the reported extent of apoptotic cells in the BM of MDS patients and the relationship between p53 gene and apoptosis, we evaluated p53 immunostaining to determine whether it can help in the differential diagnosis.…”

Section: Discussionmentioning

confidence: 99%

“…The absence of both ring sideroblasts and increased blasts, as in hypocellular refractory anemia (hypo RA), adds further difficulty [9]. Morphological evidence of bi-or trilineage dysplasia in the peripheral blood (PB) or bone marrow (BM), the presence of clonal cytogenetic abnormalities, and the pattern of CD34 immunostaining of marrow biopsies are evidence for hypo RA [10,12,14,26].…”

Section: Introductionmentioning

confidence: 99%

Significance of p53 overexpression in bone marrow biopsies from patients with bone marrow failure: aplastic anemia, hypocellular refractory anemia, and hypercellular refractory anemia

Elghetany¹,

Vyas²,

Yuoh³

1998

Annals of Hematology

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Among patients with bone marrow failure, differentiating acquired aplastic anemia (AA) from hypocellular refractory anemia (hypo RA) can be a difficult and challenging task. Morphological, cytochemical, immunocytochemical, and cytogenetic studies may provide tools for discriminating between both entities. In addition, differences in the pattern of proliferation and apoptosis of bone marrow cells in AA and in the myelodysplastic syndrome have been reported. Because of the correlation between p53 and apoptosis, we examined the overexpression of p53 on bone marrow biopsies in RA and AA. Our study included 14 patients with hypo RA, 14 patients with hypercellular (hyper) RA, ten patients with classic acquired AA, and 37 hematologically normal individuals. p53 was overexpressed in eight (57%) hypo RA patients and 11 (79%) hyper RA patients. All normal individuals and patients with AA showed no overexpression of p53 in their marrow. These results were statistically significant:p < 0.01 (AA vs hypo RA), p<0.001 (AA vs hyper RA), while the difference between hypo RA and hyper RA was not statistically significant. We conclude that p53 overexpression in bone marrow biopsies is a valuable tool for studying bone marrow failure and may provide additional information to help differentiate hypo RA from acquired AA.

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Increased intracellular activity of matrix metalloproteinases in neutrophils may be associated with delayed healing of infection without neutropenia in myelodysplastic syndromes

Yamaguchi

Ohyashiki

2005

Ann Hematol

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To investigate the influence of the intracellular activity of type II and type IV collagenases [matrix metalloproteinases (MMP)-2, MMP-8, and MMP-9] in neutrophils from patients with myelodysplastic syndromes (MDS), we tried to measure intracellular activity using flow cytometric techniques. We also studied the clinical features of patients showing high activity. The intracellular collagenase activity, expressed as a ratio to the standardized fluorescence intensity, in patients with MDS was significantly higher than normal volunteers (19.5+/-14.8 vs 13.3+/-6.8, p=0.024). The difference among subcategories of MDS according to the French-American-British (FAB) and WHO classifications was not significant. No significant influence of three variables of the International Prognostic Scoring System (IPSS) was seen on activity. Of 8 patients with activity of more than 26.9 (mean+2 standard deviations of normal controls), 5 experienced an episode of delayed healing of infection without neutropenia, while 1 of 43 patients with activity of less than 26.9 experienced such an episode (p=0.0002). The average collagenase activity of six patients with delayed healing of infection without neutropenia (44.7+/-28.9) was significantly higher than that of other MDS patients (16.0+/-7.1, p=0.005) (Fig. 4). It was also significantly higher than the activity of the control group (13.3+/-6.8, p=0.011). Our report suggests that increased collagenase activity in neutrophils may delay healing of infection. In addition, we suggest that increased collagenase activity may be an independent prognostic factor for the susceptibility to severe infection in MDS.

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Double Esterase Staining andOther Neutrophilic Granule Abnormalities in 237 Patients with the Myelodysplastic Syndrome Studied by the Cancer and Leukemia Group B

Cited by 8 publications

References 8 publications

Case Report

Case Report

Significance of p53 overexpression in bone marrow biopsies from patients with bone marrow failure: aplastic anemia, hypocellular refractory anemia, and hypercellular refractory anemia

Increased intracellular activity of matrix metalloproteinases in neutrophils may be associated with delayed healing of infection without neutropenia in myelodysplastic syndromes

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