Double helix formation from non-natural amylose analog polysaccharides

Yui, Toshifumi; Uto, Takuya; Nakauchida, Takuya; Yamamoto, Katsuhiro; Kadokawa, Jun‐ichi

doi:10.1016/j.carbpol.2018.02.026

Cited by 13 publications

(3 citation statements)

References 31 publications

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“…The analysis method presented here can also be applied to nonprotein polymer lattices that are comprised of multiple strands. For example, amylose adopts two distinct double-helical forms (A and B), as do nonnatural amylose analog polysaccharides ( 59 ). Amylose cooperatively binds aromatic molecules and fatty acids and that are widely used as fragrances and antibacterial agents in the food and cosmetic industry ( 60 ).…”

Section: Resultsmentioning

confidence: 99%

Cooperative ligand binding to a double-stranded Ising lattice—Application to cofilin binding to actin filaments

Cao,

Taylor,

De La Cruz

2023

PNAS Nexus

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Cooperative ligand binding to linear polymers is fundamental in many scientific disciplines, particularly biological and chemical physics and engineering. Such ligand binding interactions have been widely modeled using infinite one-dimensional Ising models even in cases where the linear polymers are more complex (e.g., actin filaments and other double-stranded linear polymers). Here, we use sequence generating and transfer matrix methods to obtain an analytical method for cooperative equilibrium ligand binding to double-stranded Ising lattices. We use this exact solution to evaluate binding properties and features and analyze experimental binding data of cooperative binding of the regulatory protein, cofilin, to actin filaments. This analysis, with additional experimental information about the observed bound cofilin cluster sizes and filament structure, reveals that a bound cofilin promotes cooperative binding to its longitudinal nearest neighbors but has very modest effects on lateral nearest neighbors. The bound cofilin cluster sizes calculated from the best fit parameters from the double-stranded model are considerably larger than when calculated with the 1-D model, consistent with experimental observations made by electron microscopy and fluorescence imaging. The exact solution obtained and the method for using the solution developed here can be widely used for analysis of variety of multi-stranded lattice systems.

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Section: Resultsmentioning

confidence: 99%

Cooperative ligand binding to a double-stranded Ising lattice—Application to cofilin binding to actin filaments

Cao,

Taylor,

De La Cruz

2023

PNAS Nexus

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“…The assay system of turbidity development in methanol solution would be applied not only for basic analysis of A-type crystal development but also for screening of compounds or factors affecting the crystal development, which would be potentially used for control of starch structures and functions as well as design of novel materials based on double helix structure. 24) 25) …”

Section: Resultsmentioning

confidence: 99%

Purification of Branched Dextrin from Nägeli Amylodextrin by Ethanol Precipitation and Characterization of Its Aggregation Property in Methanol-Water

et al. 2019

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Ethanol precipitation process for purification of branched dextrin (BD) in Nägeli amylodextrin from waxy rice starch was developed. Temperature and ethanol concentration for precipitation were main parameters affecting the recovery and purity of BD, and the purification condition at 4 °C and 10 % (v/v) ethanol in water was adopted. After four-time precipitation, the BD recovery was 34.6 %, whereas the purity improved from 78.5 % at the initial to 94.5 % at the four-time purified BD (BD4). BD4 mainly showed a chain length distribution between 18 to 35 with a mode length of 25, which shifted after enzymatic debranching with isoamylase to that between 9 and 20 with a mode length of 14. Each purified BD was solubilized in water, and each solution was mixed with methanolwater at 25 °C to a final methanol concentration of 16 M. The flakes of BD precipitated with 16 M methanol exhibited an A-type crystal structure by an X-ray diffraction analysis, and the speed generation of white flakes in 16 M methanol dramatically increased as the purification time increased. The effect of addition of highly branched cyclic dextrin (HBCD) or sodium tetraborate on BD aggregation in 16 M methanol was also investigated, where the former retarded aggregation but the latter had no effect on the velocity. Thus, the purified BD enables rapid characterization of aggregation of double helix structures of A-type crystal structure, and screening of compounds which could affect the phenomena for prediction of potentials in starch modification as well.

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“…The produced amylosamine shows water-solubility because it has not formed a regular higher-order assembly in water as observed for the amylose double helix. However, the cationic amylosamine formed a double helix with anionic amylouronic acid (α(1→4)-linked GlcA polymer), another amylose analog polysaccharide, by electrostatic interaction between amino and carboxylate groups [58]. Furthermore, reductive amination of amylosamine formed hierarchically controlled assemblies to obtain nanoparticles, microaggregates, and macrohydrogels depending on reaction conditions [59].…”

Section: Thermostable Gp-catalyzed Enzymatic Oligomerization and Polymentioning

confidence: 99%

α-Glucan Phosphorylase-Catalyzed Enzymatic Reactions Using Analog Substrates to Synthesize Non-Natural Oligo- and Polysaccharides

Kadokawa

2018

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As natural oligo- and polysaccharides are important biomass resources and exhibit vital biological functions, non-natural oligo- and polysaccharides with a well-defined structure can be expected to act as new functional materials with specific natures and properties. α-Glucan phosphorylase (GP) is one of the enzymes that have been used as catalysts for practical synthesis of oligo- and polysaccharides. By means of weak specificity for the recognition of substrates by GP, non-natural oligo- and polysaccharides has precisely been synthesized. GP-catalyzed enzymatic glycosylations using several analog substrates as glycosyl donors have been carried out to produce oligosaccharides having different monosaccharide residues at the non-reducing end. Glycogen, a highly branched natural polysaccharide, has been used as the polymeric glycosyl acceptor and primer for the GP-catalyzed glycosylation and polymerization to obtain glycogen-based non-natural polysaccharide materials. Under the conditions of removal of inorganic phosphate, thermostable GP-catalyzed enzymatic polymerization of analog monomers occurred to give amylose analog polysaccharides.

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Double helix formation from non-natural amylose analog polysaccharides

Cited by 13 publications

References 31 publications

Cooperative ligand binding to a double-stranded Ising lattice—Application to cofilin binding to actin filaments

Cooperative ligand binding to a double-stranded Ising lattice—Application to cofilin binding to actin filaments

Purification of Branched Dextrin from Nägeli Amylodextrin by Ethanol Precipitation and Characterization of Its Aggregation Property in Methanol-Water

α-Glucan Phosphorylase-Catalyzed Enzymatic Reactions Using Analog Substrates to Synthesize Non-Natural Oligo- and Polysaccharides

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