Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear

Kadota, Masanori; Imizu, Kiyotoshi; Hirano, Takashi

doi:10.1016/s0304-4238(00)00234-x

Cited by 54 publications

(40 citation statements)

References 28 publications

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“…Interestingly in this study, BAP was less effective than both TDZ and 2iP on the hypocotyl explants, which is in opposition to the findings of Kadota et al (2001), and Kadota and Nimi (2003) who found BAP to have more noticeable effect than TDZ on shoot multiplication of pear. It was also in contradiction with Sedlák and Paprštein (2007) who found 2iP unsuitable for shoot proliferation in sweet cherry.…”

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confidence: 99%

Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) ^|^#8220;KY Cross^|^#8221;

Ravanfar

Salim

Aziz

et al. 2014

Plant Biotechnology

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In this study, the effects of phenyl-urea (Thidiazuron) and adenine (6-benzylaminopurine) and 6-y,y,dimethylally-amino purine type cytokinins alone or in combination with indole-3-butyric acid on shoot regeneration from hypocotyl and cotyledonary explants of Brassica oleracea ssp. capitata "KY Cross" were investigated. For hypocotyl explants, medium containing 2.27 µM Thidiazuron showed the highest mean number (18.15) of separable shoots per explant with 80% shoot formation. In the case of cotyledonary explants, the highest mean number of shoots (3.03) was obtained on medium containing 12.30 µM 6-y,y,dimethylally-amino purine with a percentage of 56.67% shoot formation. Plantlets were successfully acclimatized with 70% survival in potting medium consisting of coconut husk+vermicompost (7 : 1 v/v). The regeneration system developed herewith will be a valuable tool for genetic improvement of cabbage "KY Cross".

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confidence: 99%

Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) ^|^#8220;KY Cross^|^#8221;

Ravanfar

Salim

Aziz

et al. 2014

Plant Biotechnology

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“…of plants can be propagated. To date a very few reports on DPS has been reported that has improved the efficiency of in vitro shoot development in certain plants like in Pineapple [4], in conifers [5] and in Japanese pear [6]. All these DPS study, reported same media composition (both in the semi-solid and liquid phase) responsible for increasing the efficiency of shooting only.…”

Section: Saj Biotechnology Issn: 2375-6713mentioning

confidence: 99%

A Double Phase Culture System: An Economic and Time Saving Protocol for in vitro Propagation of Plant

Senapati¹

2015

Scholarena Journal of Biotechnology

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Indiscriminate over exploitation from natural source to meet the growing demand by industry coupled with low seed viability, lack of vegetative propagation methods and insufficient attempts for replenishment of wild stock of different important plant species have contributed to its threatened status. So realizing the threat of extinction and to meet the growing needs, the alternate method for propagation is micropropagation. This technique is used not only for those plants which are difficult to be propagated through conventional practices, but also for the mass multiplication of existing stocks of germplasm for biomass production and conservation of important, elite and rare plant species that are threatened or on the verge of extinction [1]. In all reports, protocols described are based on conventional micropropagation system (CMS). This CMS explained most common methods of micropropagation (Table 1), which involve initiation/bud proliferation, the shoot multiplication followed by rooting via a semi solid medium. Although CMS have been highly successful in terms of multiplication yields, it has become increasingly important to improve productivity and uniformity of commercially important material in reduced cost, time and space [2]. Hence an improvement in plant tissue culture techniques for the mass propagation of commercial plants is highly desirable [3]. An alternative that has shown potential to improve the efficiency of protocols for in vitro multiplication is the use of a double-phasic culture system (DPS). In this method, the explants remain in the semi-solid medium and the liquid medium is added over periodically throughout the culture, thus Low cost and time saving tissue culture techniques by improving process efficiency and better utilization of resources without compromising the quality of the plants are of high priority for commercial utilization. In this report a double phase culture system (DPS) i.e. a semisolid phase with rooting media at the bottom with a layer of shooting media in liquid phase, was standardized and compared with conventional micro propagation system (CMS) by taking Rauwolfia serpentine L. Bent. as a model. In CMS the shoots were raised first followed by two subsequent subculture then they were transferred to rooting media for root induction for which it will take more than 12 weeks but under DPS, plantlets with well developed roots and shoots can be produced simultaneously in a single culture vessel within 08 weeks without shoot manipulations/repeated subculture. It was found that the rate of multiplication (98.7), shoot length (9.8) and shoot thickness (5.2) was higher in DPS in comparison to CMS. The no. of roots/shoot (8.7), root length (5.3cm), root thickness (6.1mm) was found higher in DPS than that of the CMS. Percentage survival of plantlets was 98.5 % in DPS, while it was only 70% in CMS. The culture period was reduced by 44% in DPS compared to the CMS. In DPS nutrient cost/plantlet, energy cost/plantlet and labour cost also reduced by 35.36%, 40.66% and 33.33% respe...

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“…These findings are in conformity with those of in P. pyrifolia cv. in Japanese pear cultivar Hosui, who reported superiority of WPM over other media with respect to proliferation rate [19,20]. BAP level was found to effect significantly per cent proliferation, shoots per explant and shoot length and these results are in conformity with studies reported by [19]; [22]; Karimpour et al [18]; Hassanen and Gabr [2]; Ruzic et al [7]; Isikalan et al [23] and Soni et al [24].…”

Section: Shoot Proliferationmentioning

confidence: 99%

In vitro Propagation of Kainth (Pyrus pashia) Using Explants from Forced Cutting

2015

Horticulture

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This study was carried out in Tissue culture laboratory, Department of Fruit Science, Punjab Agricultural University, Ludhiana during 2011-13. The effect of various media {1/2 MS (M 1 ), MS (M 2 ) and WPM (M 3 )} and growth regulators (BAP, IBA and NAA) on establishment, proliferation and rooting was studied. Maximum establishment (63.60%) was obtained on M 2 containing BAP (1.5 mgl -1 ) and IBA (0.25 mgl -1 ). Maximum proliferated cultures (95.30%) and shoots per explant were obtained using M 3 medium fortified with BAP (3.0 mgl -1 ). However, shoots of maximum length (42.97 mm) were obtained in M 3 medium containing BAP (0.0 mgl-1) i.e control. Rooting (%), roots per explant and root length was found to be influenced by type of medium and growth regulator fortification. Rooting (%) was maximum (13.34%) was observed in M 1 medium supplemented with IBA (0.1 mgl -1 ). However, NAA (1.0 mgl -1 ) induced rooting of 29.61 per cent using M 1 medium. Maximum roots per explant were obtained using M 1 medium supplemented with IBA (1.0 mgl -1 ). NAA (1.0 mgl -1 ) induced highest roots per explant i.e 3.40 using M 1 medium. However, M 3 supplemented with IBA (0.1 mgl -1 ) resulted in maximum root length of 31.15 mm. NAA (0.1 mgl -1 ) resulted in maximum root length of 22.97 mm using M 1 medium.

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Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear

Cited by 54 publications

References 28 publications

Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) ^|^#8220;KY Cross^|^#8221;

Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) ^|^#8220;KY Cross^|^#8221;

A Double Phase Culture System: An Economic and Time Saving Protocol for in vitro Propagation of Plant

In vitro Propagation of Kainth (Pyrus pashia) Using Explants from Forced Cutting

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