Double-Quantum Excitation in the NMR of Spinning Solids by Pulse-Assisted Rotational Resonance

Karlsson, Torgny; Hughes, Colan E.; Günne, Jörn Schmedt auf der; Levitt, Malcolm H.

doi:10.1006/jmre.2000.2243

Cited by 8 publications

(10 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, many previous studies have also used analytical simulations incorporating phenomenological transverse relaxation of the observed signal20,21 for data fitting; the work of numerous investigators51,52 including ourselves on this subject30,53 demonstrate the importance of multi-quantum relaxation parameters in MAS NMR distance measurements. As we show in Figure 2, however, the reduced sensitivity of DQ DRAWS to these effects is still a profound advantage.…”

Section: Resultsmentioning

confidence: 99%

Accurate Determination of Interstrand Distances and Alignment in Amyloid Fibrils by Magic Angle Spinning NMR

et al. 2010

View full text Add to dashboard Cite

Amyloid fibrils are structurally ordered aggregates of proteins whose formation is associated with many neurodegenerative and other diseases. For that reason, their high resolution structures are of considerable interest and have been studied using a wide range of techniques, notably electron microscopy, x-ray diffraction, and magic angle spinning (MAS) NMR. Because of the excellent resolution in the spectra, MAS NMR is uniquely capable of delivering site-specific, atomic resolution information about all levels of amyloid structure: (1) the monomer, which packs into several (2) protofilaments that in turn associate to form a (3) fibril. Building upon our high resolution structure of the monomer of an amyloid-forming peptide from transthyretin (TTR 105-115 ), we introduce single 1-13 C labeled amino acids at seven different sites in the peptide and measure intermolecular carbonyl-carbonyl distances with an accuracy of ~0.11 A. Our results conclusively establish a parallel, in register, topology for the packing of this peptide into a β-sheet and provide constraints essential for the determination of an atomic resolution structure of the fibril. Furthermore, the approach we employ, based on a combination of a double-quantum filtered variant of the DRAWS recoupling sequence and multispin numerical simulations in SPINEVOLUTION, is general and should be applicable to a wide range of systems.

show abstract

Section: Resultsmentioning

confidence: 99%

Accurate Determination of Interstrand Distances and Alignment in Amyloid Fibrils by Magic Angle Spinning NMR

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Possible applications of the zero-quantum recoupling sequence SR6 2 6 include the quantitative estimation of internuclear distances (8,(62)(63)(64)(65)(66) and excitation of multiple-quantum coherences (9,58,63,(67)(68)(69)(70). Recently, the excitation of highorder 13 C multiple-quantum coherence in magic-angle-spinning solids was demonstrated using the fpRFDR sequence (70).…”

Section: Discussionmentioning

confidence: 99%

Homonuclear Zero-Quantum Recoupling in Fast Magic-Angle Spinning Nuclear Magnetic Resonance

Brinkmann

Günne

Levitt

2002

Journal of Magnetic Resonance

141

View full text Add to dashboard Cite

“…Although the sensitivity achieved in this first experiment is relatively poor, advances in dipolar recoupling methodology are likely to improve matters in the future (32,33,(40)(41)(42). The conformational distributions that are likely to be encountered in complex systems will create complications, but these might be resolved in an informative way by combining several types of complementary NMR data.…”

Section: Discussionmentioning

confidence: 94%

Conformation of the Glycosidic Linkage in a Disaccharide Investigated by Double-Quantum Solid-State NMR

Ravindranathan

Karlsson

Lycknert³

et al. 2001

Journal of Magnetic Resonance

Self Cite

View full text Add to dashboard Cite

Double-Quantum Excitation in the NMR of Spinning Solids by Pulse-Assisted Rotational Resonance

Cited by 8 publications

References 48 publications

Accurate Determination of Interstrand Distances and Alignment in Amyloid Fibrils by Magic Angle Spinning NMR

Accurate Determination of Interstrand Distances and Alignment in Amyloid Fibrils by Magic Angle Spinning NMR

Homonuclear Zero-Quantum Recoupling in Fast Magic-Angle Spinning Nuclear Magnetic Resonance

Conformation of the Glycosidic Linkage in a Disaccharide Investigated by Double-Quantum Solid-State NMR

Contact Info

Product

Resources

About