Double-sided battle: The role of ethylene during Monilinia spp. infection in peach at different phenological stages

Baró‐Montel, Núria; Vall‐llaura, Núria; Giné-Bordonaba, Jordi; Usall, J.; Serrano-Prieto, Sandra; Teixidó, Neus

doi:10.1016/j.plaphy.2019.09.048

Cited by 17 publications

(18 citation statements)

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“…Along this line, ACS2 and ACO1 , involved in system 2 ET production 32 , were overexpressed in inoculated immature tissues. Previous studies have reported on a similar modulation of ET biosynthesis by the pathogen 33 . However, after 24 hpi, ethylene levels in inoculated immature fruit fell to control levels, and the fruit remained resistant.…”

Section: Discussionsupporting

confidence: 61%

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Depicting the battle between nectarine and Monilinia laxa: the fruit developmental stage dictates the effectiveness of the host defenses and the pathogen’s infection strategies

et al. 2020

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Infections by the fungus Monilinia laxa, the main cause of brown rot in Europe, result in considerable losses of stone fruit. Herein, we present a comprehensive transcriptomic approach to unravel strategies deployed by nectarine fruit and M. laxa during their interaction. We used M. laxa-inoculated immature and mature fruit, which was resistant and susceptible to brown rot, respectively, to perform a dual RNA-Seq analysis. In immature fruit, host responses, pathogen biomass, and pathogen transcriptional activity peaked at 14–24 h post inoculation (hpi), at which point M. laxa appeared to switch its transcriptional response to either quiescence or death. Mature fruit experienced an exponential increase in host and pathogen activity beginning at 6 hpi. Functional analyses in both host and pathogen highlighted differences in stage-dependent strategies. For example, in immature fruit, M. laxa unsuccessfully employed carbohydrate-active enzymes (CAZymes) for penetration, which the fruit was able to combat with tightly regulated hormone responses and an oxidative burst that challenged the pathogen’s survival at later time points. In contrast, in mature fruit, M. laxa was more dependent on proteolytic effectors than CAZymes, and was able to invest in filamentous growth early during the interaction. Hormone analyses of mature fruit infected with M. laxa indicated that, while jasmonic acid activity was likely useful for defense, high ethylene activity may have promoted susceptibility through the induction of ripening processes. Lastly, we identified M. laxa genes that were highly induced in both quiescent and active infections and may serve as targets for control of brown rot.

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Section: Discussionsupporting

confidence: 61%

“…For symptom analysis, inoculated fruit was imaged at the set time points. Ethylene production of both mock and M. laxa inoculated immature and mature fruit was determined, as described by Baró-Montel 33 .…”

Section: Methodsmentioning

confidence: 99%

Depicting the battle between nectarine and Monilinia laxa: the fruit developmental stage dictates the effectiveness of the host defenses and the pathogen’s infection strategies

et al. 2020

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“…However, in a recent study aiming to explore the role of ethylene during Monilinia spp. infection has been proposed that brown rot agents try to suppress ethylene biosynthesis aiming to an inhibition of fruit defense reactions 62 . The different level of 1-aminocyclopropane-1-carboxylate deaminase in the present work might be due to the spatial and temporal action of ethylene that was produced both during fruit-pathogen interactions and peach fruit ripening process.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis upon peach fruit infection with Monilinia fructicola and M. laxa identify responses contributing to brown rot resistance

Papavasileiou

Tanou²,

Samaras

et al. 2020

Sci Rep

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Brown rot, caused by Monilinia spp., is a major peach disease worldwide. in this study, the response of peach cultivars Royal Glory (RG) and Rich Lady (RL) to infection by Monilinia fructicola or Monilinia laxa, was characterized. Phenotypic data, after artificial inoculations, revealed that 'RL' was relatively susceptible whereas 'RG' was moderately resistant to Monilinia spp. comparative proteomic analysis identified mesocarp proteins of the 2 cultivars whose accumulation were altered by the 2 Monilinia species. Functional analysis indicated that pathogen-affected proteins in 'RG' were mainly involved in energy and metabolism, while, differentially accumulated proteins by the pathogen presence in 'RL' were involved in disease/defense and metabolism. A higher number of proteins was differentiated in 'RG' fruit compared to 'RL'. Upon Monilinia spp. infection, various proteins were-down accumulated in 'RL' fruit. Protein identification by mass spectrometric analysis revealed that several defense-related proteins including thaumatin, formate dehydrogenase, S-formylglutathione hydrolase, cBS domaincontaining protein, HSP70, and glutathione S-transferase were up-accumulated in 'RG' fruit following inoculation. The expression profile of selected defense-related genes, such as major latex allergen, 1-aminocyclopropane-1-carboxylate deaminase and UDP-glycoltransferase was assessed by RT-PCR. This is the first study deciphering differential regulations of peach fruit proteome upon Monilinia infection elucidating resistance responses.

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“…Similarly, an inhibitory effect of chlorogenic and neochlorogenic acids, compounds that tend to be higher in unripe fruit (Bostock et al, 1999), had been reported as crucial for M. laxa pathogenicity by interfering with fungal melanin biosynthesis (Villarino et al, 2011). Regarding the molecular determinants of the fruit ripening-associated changes in brown rot susceptibility, scarce information is relatively available (Baro´-Montel et al, 2019). Guidarelli et al (2014) compared the gene expression profile between susceptible (two weeks before the pit hardening) and resistant (pit hardening) peach fruit developmental stages, finding noteworthy changes in phenylpropanoid and jasmonate-related genes.…”

Section: Introductionmentioning

confidence: 99%

“…Guidarelli et al (2014) compared the gene expression profile between susceptible (two weeks before the pit hardening) and resistant (pit hardening) peach fruit developmental stages, finding noteworthy changes in phenylpropanoid and jasmonate-related genes. In addition, a recent study has pointed out the differential role that ethylene plays in the interaction of Monilinia spp.-stone fruit (Baro´-Montel et al, 2019). Accordingly, ethylene might have an effect not only on inducing defence responses to both abiotic and biotic stress in the plant, but on promoting susceptibility to certain fruit pathogens (Chague´et al, 2006;Shigenaga and Argueso, 2016).…”

Section: Introductionmentioning

confidence: 99%

Scrutinising the relationship between major physiological and compositional changes during ‘Merrill O’Henry’ peach growth with brown rot susceptibility

Baró‐Montel

Giné-Bordonaba

Vall‐llaura

et al. 2020

Food sci. technol. int.

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In the present work, the major physiological and compositional changes occurring during ‘Merrill O’Henry’ peach growth and its relationship with susceptibility to three strains of Monilinia spp. at 49, 77, 126 and 160 days after full bloom were explored. Results of disease incidence indicated wide differences among phenological stages, being 49 and 126 days after full bloom the moment when peaches showed significantly lower susceptibility to brown rot (40 and 23% of rotten fruit, respectively, for strain ML8L). Variation in brown rot susceptibility among different growth stages was also strain-dependent. Lower fruit susceptibility to ML8L at 49 and 126 was accompanied by noticeable changes in the fruit ethylene and respiration patterns, and also in sugars and organic acids content. By employing a partial least squares regression model, a strong negative relationship between citric acid, and a positive association of ethylene with peach susceptibility to Monilinia spp. at diverse phenological stages were observed. The results obtained herein highlight that the content of certain compounds such as citrate, malate and sucrose; the respiratory activity and the fruit ethylene production may mediate in a coordinated manner the fruit resistance to Monilinia spp. at different phenological stages of peach fruit.

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Double-sided battle: The role of ethylene during Monilinia spp. infection in peach at different phenological stages

Cited by 17 publications

References 50 publications

Depicting the battle between nectarine and Monilinia laxa: the fruit developmental stage dictates the effectiveness of the host defenses and the pathogen’s infection strategies

Depicting the battle between nectarine and Monilinia laxa: the fruit developmental stage dictates the effectiveness of the host defenses and the pathogen’s infection strategies

Proteomic analysis upon peach fruit infection with Monilinia fructicola and M. laxa identify responses contributing to brown rot resistance

Scrutinising the relationship between major physiological and compositional changes during ‘Merrill O’Henry’ peach growth with brown rot susceptibility

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