Double-strand RNAs targeting MaltRelish and MaltSpz reveals potential targets for pest management of Monochamus alternatus

Chu, Xiangxiang; Yang, Meijiao; Yu, Lu; Xie, Hongyun; Liu, Jinyan; Wu, Songqing; Zhang, Feiping; Hu, Xia

doi:10.1016/j.pestbp.2023.105495

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…These results are consistent with some previous studies; for example, microinjection of dsRelish in Monochamus alternatus significantly increased the mortality of larvae infected by Beauveria bassiana. 34 In T. molitor, dsRelish led to significantly higher mortality in larvae following Listeria monocytogenes infection. 26 In general, Relish could regulate the expression of AMPs through the IMD pathway.…”

Section: Discussionmentioning

confidence: 97%

“…In M. alternatus, the expression level of Att-1 was significantly increased at 6 and 12 h, but significantly reduced at 24 h. No significant differences were observed for the Att-2 and Def-1 following the knockdown of Relish. 34 In Phlebotomus duboscqi, a significant reduction was observed in the expression levels of Def2 and Att genes. 23 Our results indicated that Relish is crucial for the IMD pathway mediating the expression of defensin1, defensin2, and attacin2 in P. versicolora.…”

Section: Discussionmentioning

confidence: 99%

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Relish involved in immunity and larval survival in the willow leaf beetle Plagiodera versicolora

Liu,

Zhang,

Wang

et al. 2024

Pest Management Science

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BACKGROUNDInsects mainly rely on innate immunity against pathogen infection. Plagiodera versicolora (Coleoptera: Chrysomelidae), is a worldwide leaf‐eating forest pest in salicaceous trees. However, the mechanisms of the immunodeficiency pathway (IMD) are still poorly understood.RESULTSIn this study, we obtained a Relish gene from our transcriptome analysis. Subsequently, the tissue and instar expression profiles were performed by quantitative real‐time PCR. The results showed that Relish has highly expressed levels in eggs, larvae and adults, and especially in fat bodies. The transcripts of the tested antimicrobial peptides (AMPs), defensin1, defensin2 and attacin2 were downregulated by dsRelish. Knockdown of Relish led to a higher mortality in larvae after Staphylococcus aureus infection. In addition, we performed the bacterial 16S rRNA‐based high throughput sequencing. The results showed that the relative abundance of some gut bacteria was significantly altered after dsRelish ingestion.CONCLUSIONThis study would help further to understand of IMD signaling pathway, facilitating functional studies of the Relish in P. versicolora. Moreover, genetic pest management technique might be develop using Relish as a lethal gene to control the pest of P. versicolora.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Relish involved in immunity and larval survival in the willow leaf beetle Plagiodera versicolora

Liu,

Zhang,

Wang

et al. 2024

Pest Management Science

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Identification of pine wood nematode (Bursaphelenchus xylophilus) loading response genes in Japanese pine sawyer (Monochamus alternatus) through comparative genomics and transcriptomics

Zou,

Jia,

Zhu

et al. 2024

Pest Management Science

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BACKGROUNDPine wood nematode (PWN; Bursaphelenchus xylophilus) is the causative agent of pine wilt disease (PWD), which is considered the most dangerous biohazard to conifer trees globally. The transmission of PWN relies on insect vectors, particularly the Japanese pine sawyer (JPS; Monochamus alternatus). However, the molecular mechanism underlying PWN‐JPS assembly remains largely unknown.RESULTSHere, we found that both geographical and gender could significantly affect the PCA (PWN carrying amount) of JPS; thus, JPS transcriptomes from diverse locations and genders were explored regard to PWN loading. Due to the shortage of genomes, we developed a full‐length reference transcriptome for analyzing next‐generation sequencing data. A comparative genomic study was performed, and 11 248 potential PWN‐carrying associate genes (β) were nominated in JPS by using the reported genomes of PWN and non‐PWN carrier insect species. Then, 151 differentially expressed transcripts (DETs), 28 of them overlapped with β, correlated with the PCA of JPS were nominated by RNA‐Seq, and found that fatty acid β‐oxidation might be the key factor that affected the PCA of JPS. Furthermore, JPS fatty acid β‐oxidation rates were experimentally decreased using the inhibitor Etomoxir, leading to an increased PCA of JPS. Meanwhile, silencing MaCPT1 in JPS by RNA interference led to a decreased fatty acid β‐oxidation rate and increased PCA of JPS.CONCLUSIONSIn conclusion, MaCPT1 was able to decrease the PWN‐JPS assembly formation through the fatty acid β‐oxidation of JPS. These results provide new insights for exploring the impact of PWN invasion on JPS.This article is protected by copyright. All rights reserved.

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RNAi-mediated silencing of NlGRP3 augments the insecticidal virulence of Metarhizium anisopliae to the brown planthopper Nilaparvata lugens

Shao,

Lan,

et al. 2024

Pesticide Biochemistry and Physiology

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Double-strand RNAs targeting MaltRelish and MaltSpz reveals potential targets for pest management of Monochamus alternatus

Cited by 3 publications

References 35 publications

Relish involved in immunity and larval survival in the willow leaf beetle Plagiodera versicolora

Relish involved in immunity and larval survival in the willow leaf beetle Plagiodera versicolora

Identification of pine wood nematode (Bursaphelenchus xylophilus) loading response genes in Japanese pine sawyer (Monochamus alternatus) through comparative genomics and transcriptomics

RNAi-mediated silencing of NlGRP3 augments the insecticidal virulence of Metarhizium anisopliae to the brown planthopper Nilaparvata lugens

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