1997
DOI: 10.1006/viro.1997.8664
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Double-Stranded RNA-Dependent Protein Kinase (PKR) Is Regulated by Reovirus Structural Proteins

Abstract: Reovirus sigma3 is a virion outer shell protein that also binds dsRNA and stimulates translation by blocking activation of the dsRNA-dependent protein kinase, PKR. Purified sigma3 was shown by gel shift assay to bind specifically to RNA duplexes of minimal length 32-45 base pairs. PKR binding to dsRNA was prevented by sigma3, and translation inhibition of luciferase reporter by PKR expression in transfected cells was reversed by sigma3. Association of sigma3 with its outer capsid partner mu1/mu1C eliminated ds… Show more

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Cited by 106 publications
(94 citation statements)
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“…At 8 h after infection, luciferase expression levels were measured. Expression of PKR reduced luciferase synthesis, in accordance with previous studies (28,(31)(32)(33) (Fig. 4A).…”
Section: Sumo Potentiates the Control Of Protein Synthesis By Pkr-supporting
confidence: 92%
“…At 8 h after infection, luciferase expression levels were measured. Expression of PKR reduced luciferase synthesis, in accordance with previous studies (28,(31)(32)(33) (Fig. 4A).…”
Section: Sumo Potentiates the Control Of Protein Synthesis By Pkr-supporting
confidence: 92%
“…For example, poliovirus infection results in the degradation of PKR (6,7). Several viruses block activation of PKR by sequestering dsRNA or by interfering with PKR dimerization or enzymatic activity (26,27,32,64,65,72). The herpes simplex virus ␥34.5 gene product blocks the effects of PKR by cooperating with cellular protein phosphatase 1␣ to dephosphorylate eIF2␣-phosphate (37).…”
Section: Discussionmentioning
confidence: 99%
“…Escherichia coli RNase III and the mammalian reovirus outer shell polypeptide s3 are among the best-characterized dsRBPs; therefore, we tested the RNAi suppressor capacity of these proteins and their mutants. Both proteins carry conservative dsRNA-binding motifs and bind dsRNAs in vitro and in vivo (Dasgupta et al, 1998;Denzler & Jacobs, 1994;Fierro-Monti & Mathews, 2000;Huismans & Joklik, 1976;Kharrat et al, 1995;Nicholson, 1999;Yue & Shatkin, 1997). The postulated silencing suppressor capacity of E. coli RNase III, a mutant RNase III that binds dsRNA but lacks RNA cleavage activity (Rnc70) (Dasgupta et al, 1998) and reovirus s3 proteins were tested in the Agrobacterium co-infiltration assay.…”
mentioning
confidence: 99%
“…than 32-45 bp (Yue & Shatkin, 1997). Because RNase III cleaves structured ssRNAs (Nicholson, 1999), it might also bind miRNA precursors, thereby reducing the accumulation of miRNAs.…”
mentioning
confidence: 99%