Double‐Stranded DNA‐Templated Copper Nanoclusters for Detection of DNA Polymerase Activity

Abstract

DNA polymerase plays an important role in the proliferation and survival of all forms of life and the variety of biotechnological applications. In this study, DNA‐templated copper nanoclusters were utilized to detect Klenow fragment exo‐ (KF‐) activity and monitor its inhibition in the presence of 5‐fluorouracil by measurement of fluorescence intensities. This study showed that the fluorescence intensities increased continuously for KF‐ concentrations ranging from 0 to 5 U/mL. In addition, inhibition of KF‐ activity was measured at 5‐fluorouracil concentrations ranging from 0 to 150 μM. Using nonlinear regression, the corresponding IC50 was determined to be 6.23 μM in the presence of 5.0 U/mL KF‐.

Design and Synthesis of Novel N‐(2‐aminophenyl)benzamide Derivatives as Histone Deacetylase Inhibitors and Their Antitumor Activity Study

“…Many cancers are associated with epigenetic mechanisms 1–3 . Post‐translational modifications of histones regulate gene transcription have been widely recognized to have a large impact on genetic diseases 4–6 .…”

“…Many cancers are associated with epigenetic mechanisms. [1][2][3] Post-translational modifications of histones regulate gene transcription have been widely recognized to have a large impact on genetic diseases. [4][5][6] Histone deacetylases (HDACs) play crucial roles in epigenetic regulation of genes.…”

Facile Strategy to Output Fluorescein from Nucleic Acid Interactions

“…In circuit construction, various outputs are connected to inputs for functionality. For instance, fluorescent, ,− bioluminescent, − colorimetric, − and electrochemical − outputs have been used to detect bioanalytes. In addition, the release of bioactive molecules, ,− regulation of protein binding, − and activation of enzymes − have been adopted as outputs of preliminary circuit models for therapeutic applications.…”

Conjugation strategies of DNA to gold nanoparticles

“…There are various methods to conjugate DNA strands to metal NPs, which should be adequately employed to fit the working condition. With the rapid advances in bioconjugation chemistry for NPs, many bioconjugation methods have been well established for DNA, antibody, and proteins to modify them to various metal NPs 15–18 . For noble metal NPs, thiol–metal bond (especially thiol–Au bond) is known as a semi‐covalent bond with bonding energy of ~45 kcal/mol 19 and is widely used to stably introduce various ligands with thiol functional groups to the NP surface.…”

“…With the rapid advances in bioconjugation chemistry for NPs, many bioconjugation methods have been well established for DNA, antibody, and proteins to modify them to various metal NPs. [15][16][17][18] For noble metal NPs, thiol-metal bond (especially thiol-Au bond) is known as a semi-covalent bond with bonding energy of $45 kcal/mol 19 and is widely used to stably introduce various ligands with thiol functional groups to the NP surface. However, the strength of a thiol-metal bond depends on many factors including pH, ionic strength, number of thiol groups, other thiol molecules, and metal species, and the stability of this bond should be carefully scrutinized depending on experimental conditions.…”