Double‐stranded <scp>RNA</scp> delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, <i>Diaphorina citri</i>

BACKGROUND The oriental fruit fly Bactrocera dorsalis (Hendel), a widespread agricultural pest, has evolved resistance to many insecticides, including organophosphorus compounds. Glutathione S‐transferases (GSTs) are involved in xenobiotic detoxification and insecticide resistance in many insects. However, the role of delta class GSTs in detoxifying malathion in B. dorsalis is unknown. Here, we evaluated the roles of two delta class GSTs in malathion detoxification in this species. RESULTS Two delta class GSTs genes, BdGSTd1 and BdGSTd10, were characterized in B. dorsalis. They were highly expressed in 5‐day‐old adults, as well as in midgut and Malpighian tubules. Upon malathion exposure, the two genes were upregulated by 2.63‐ and 2.85‐fold, respectively. Injection of double‐stranded RNA targeting BdGSTd1 or BdGSTd10 significantly reduced their mRNA levels in adults and also significantly increased adult susceptibility to malathion. The expression of these two GSTs in Escherichia coli helped the host to endure malathion stress at a concentration of 10 µg mL−1 according to a Cell Counting Kit‐8 assay. High‐performance liquid chromatography analyses indicated that malathion could be significantly depleted by the two delta GSTs. The role of BdGSTd10 in malathion sequestration was also discussed. CONCLUSION BdGSTd1 and BdGSTd10 play important roles in the detoxification of malathion in B. dorsalis. © 2019 Society of Chemical Industry

Section: Discussionmentioning

confidence: 99%

Two delta class glutathione S‐transferases involved in the detoxification of malathion in Bactrocera dorsalis (Hendel)

Meng

Yuan

et al. 2019

“…RNAi has quickly become an important tool for studying gene function in insects, including D. citri . Application of RNAi in insects is dependent upon an efficient delivery system of the dsRNA trigger.…”

Section: Discussionmentioning

confidence: 99%

RNA interference of two glutathione S‐transferase genes, Diaphorina citriDcGSTe2 and DcGSTd1, increases the susceptibility of Asian citrus psyllid (Hemiptera: Liviidae) to the pesticides fenpropathrin and thiamethoxam

Killiny

2017

Self Cite

“…Relative to injections, this would be labor‐saving and can allow for high‐throughput gene screening. However, only a few publications have shown promising results using this approach . One of the few examples includes studies conducted in adult Diaphorina citri , in which dsRNA solutions targeting five cytochrome P450 genes was applied topically into the thoracic region.…”

Section: Introductionmentioning

confidence: 99%

Prospects, challenges and current status of RNAi through insect feeding

Kunte

McGraw

Bell

et al. 2019

135

134

RNA interference is a phenomenon in which the introduction of double‐stranded RNA (dsRNA) into cells triggers the degradation of the complementary messenger RNA in a sequence‐specific manner. Suppressing expression of vital genes could lead to insect death, therefore this technology has been considered as a potential strategy for insect pest control. There are three main routes of dsRNA administration into insects: (i) injections to the hemolymph, (ii) topical, and (iii) feeding. In this review, we focus on dsRNA administration through feeding. We summarize novel strategies that have been developed to improve the efficacy of this method, such as the use of nano‐based formulations, engineered microorganisms, and transgenic plants. We also expose the hurdles that have to be overcome in order to use this technique as a reliable pest management method. © 2019 Society of Chemical Industry