Double strandedness of nascent DNA of a higher plant (Vicia faba)

Some Aspects of Eukaryotic DNA Replication

“…560 X 106 daltons; 90 X 104 base pairs, with a majority being 10-100 /Lm. This generalization derives fr om studies with yeast (4-6, 111), eukaryotic microorganisms generally (7, 12, 13, 112-1 16), higher plants (9,(117)(118)(119)(120)(121)(122), insects (10,(123)(124)(125), fi sh (1 1), birds (1 1, 13), amphibia (1 1, 126), and a broad spectrum of mammalian cells (12,13,30,109,110, and their viruses (148)(149)(150)(151)(152)(153)(154)(155)(156). A homogeneous RU of 6.93 /Lm has been identified within the "satellite" DNA of kangaroo rat cells (157).…”

“…Much of the earlier work was taken to support this hypothesis. However, the more recent in vivo studies un equivocally reveal discontinuous DNA synthesis on both template strands in yeast (4), other eukaryotic microorganisms (12,13), higher plants (117)(118)(119)(120)(121), insects (10,123,125), and a variety of mammalian cells and their viruses (9, 12, 13, 109, 127-1 34, 140, 141, 143-155). Analyses with in vitro systems similarly reveal totally discontinuous synthesis of the DNA of mammalian cells (132, 210--212) and polyoma virus (2 13, 214).…”

Differences in timing of replication and maturation of nascent DNA fragments between satellite and main band DNA during the S phase of the first cell cycle in cultured carrot root explants.

“…When embryonic axes, detached from germinating Vicia faba seeds, were pulse-labeled with 3H-thymidine, short and single-stranded DNA molecules of 5-6S and 8-9S (or lOS) appeared (Sakamaki et al, 1975(Sakamaki et al, , 1976Yakura and Tanifuji, 1980). Such short nascent DNA fragments have been observed also in radicles of germinating cotton seeds (Clay et al, 1976), root tips of pea seedlings (Schvartzman et al, 1981), and cultured soybean cells (Cress et al, 1978).…”